Surface protein Adr2 of Rickettsia rickettsii induced protective immunity against Rocky Mountain spotted fever in C3H/HeN mice

Gong, Wenping; Xiong, Xiaolu; Qi, Yong; Jiao, Jun; Duan, Changsong; Wen, Bin

doi:10.1016/j.vaccine.2014.02.057

Cited by 27 publications

(37 citation statements)

References 45 publications

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“…Recombinant protein rAdr2 was prepared as previously described [16]. The gene fragment encoding recombinant OmpB-4 was amplified by PCR from genomic DNA of R. rickettsii with a forward primer (5 -GG-GGATCC(BamHI)AGAGGCATTCCATTCA-3 ) and a reverse primer (5 -GG-CTCGAG(XhoI)-GTTCTGATCTACACCCG-3 ) designed based on a gene sequence (GenBank accession number: CP000848), and a OmpB-4-expressed strain of Escherichia coli was constructed according to conventional procedures [21].…”

Section: Preparation Of Recombinant Proteinsmentioning

confidence: 99%

“…CD4 + or CD8 + T cells were isolated from R. rickettsii-infected or non-infected mice according to the previous methods [16]. One milliliter of cell suspension containing 6 × 10 5 CD4 + or CD8 + T cells and 3 × 10 5 antigen-presenting cells isolated from naïve mouse spleens were mixed in each well of 24-well plates.…”

Section: Cytokine Determinationmentioning

confidence: 99%

“…Outer membrane protein B (OmpB), a major surface protein of R. rickettsii, has been demonstrated to be an important protective antigen [14,15]. Rickettsial adhesion 2 (Adr2) has been recognized as a surface-exposed protein and protective antigen of R. rickettsii [16,17]. Both OmpB and Adr2 are capable of inducing specific immunoresponses in animal models [16,18].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhanced protection against Rickettsia rickettsii infection in C3H/HeN mice by immunization with a combination of a recombinant adhesin rAdr2 and a protein fragment rOmpB-4 derived from outer membrane protein B

Gong

Wang

Xiong

et al. 2015

Vaccine

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Section: Preparation Of Recombinant Proteinsmentioning

confidence: 99%

Section: Cytokine Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced protection against Rickettsia rickettsii infection in C3H/HeN mice by immunization with a combination of a recombinant adhesin rAdr2 and a protein fragment rOmpB-4 derived from outer membrane protein B

Gong

Wang

Xiong

et al. 2015

Vaccine

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“…We have demonstrated that both SFG and TG rickettsial species likely possess redundancy in the mechanisms by which this class of pathogens subverts complement-mediated killing [7]. Elucidating the various strategies evolved by pathogenic bacteria in order to evade this innate immune clearance from the blood circulation may reveal conserved proteins that could function as candidates for therapeutic intervention, which has recently been described [13, 14]. …”

Section: Discussionmentioning

confidence: 99%

Expression of Rickettsia Adr2 protein in E. coli is sufficient to promote resistance to complement-mediated killing, but not adherence to mammalian cells

2017

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Bacteria exposed to host serum are subject to the antibacterial effects to the complement system. However, pathogenic microorganisms have evolved mechanisms of evading this immune attack. We have previously demonstrated that at least two R. conorii antigens, RC1281/Adr1 and OmpB β-peptide, contribute to the evasion of complement-mediated killing by binding the complement regulatory proteins vitronectin and factor H. RC1282/Adr2, a protein related to Adr1, is predicted to share similar structural features, suggesting that this protein may also contribute to evasion of complement-mediated killing. Interestingly, the R. prowazekii Adr1 and Adr2(RP828) proteins were originally found to interact with host cell surface proteins, suggesting their putative roles as adhesins in this pathogenic rickettsial species. In this study, we expressed both R. conorii and R. prowazekii Adr2 on the surface of a non-adherent, serum-sensitive strain of E. coli to examine the potential role of this protein to mediate evasion of complement-mediated killing and adherence to host cells. We demonstrate that, similar to R. conorii Adr1, R. conorii and R. prowazekii Adr2 are sufficient to mediate serum resistance and to promote interaction with the host complement regulator vitronectin. Furthermore, we demonstrate that expression of Adr2 in a non-adherent strain of E. coli is insufficient to mediate adherence to cultured mammalian endothelial cells. Together, our data demonstrate that the R. conorii and R. prowazekii Adr2 protein does not participate in the interactions with mammalian cells, but rather, participates in the evasion of killing by complement.

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“…Los estudios actuales en el desarrollo de vacunas se han enfocado en las rickettsias pertenecientes al grupo de las fiebres manchadas, debido a que éstas son las más severas. Hasta el momento, se han evaluado proteínas implicadas en la invasión celular (InvA), proteínas de secreción (Fts), Adr2, Sca5, YbgF y TolC (14)(15)(16). Sin embargo las más estudiadas son las proteínas transmembranales OmpA y OmpB, debido a su alta antigenicidad y su importante papel en el mecanismo de patogenicidad de las bacterias del género Rickettsia (12,17,18).…”

Section: Introductionunclassified

Importancia de las proteínas OmpA y OmpB en el desarrollo de vacunas contra la rickettsiosis

Martinez-Miranda¹,

Balam-Romero²,

Dzul-Rosado³

2019

Rev Biomed

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Las Rickettsiosis son un grupo de enfermedades zoonóticas causadas por las bacterias del género Rickettsia. Transmitidas por vectores ectoparásitos hematófagos como garrapatas, piojos, pulgas y ácaros, cuya incidencia ha aumentado en los últimos años. Debido a su cuadro clínico inespecífico es subdiagnosticada y confundida con otras de mayor influencia. Debido a su creciente incidencia se han estudiado antígenos rickettsiales, asi como la respuesta inmune que generan, para poder desarrollar una vacuna que permita combatir el número de casos de rickettsiosis. De estos estudios se han identificado diversas proteínas de membrana como candidatas para la realización de vacunas, siendo OmpA y la OmpB las más destacadas. Recientes estudios destacan una respuesta inmune efectiva contra esta enfermedad por lo que la presente revisión tiene como objetivo brindar un panorama de los resultados obtenidos en estudios de vacunas generadas a partir de estas dos proteínas.

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Surface protein Adr2 of Rickettsia rickettsii induced protective immunity against Rocky Mountain spotted fever in C3H/HeN mice

Cited by 27 publications

References 45 publications

Enhanced protection against Rickettsia rickettsii infection in C3H/HeN mice by immunization with a combination of a recombinant adhesin rAdr2 and a protein fragment rOmpB-4 derived from outer membrane protein B

Enhanced protection against Rickettsia rickettsii infection in C3H/HeN mice by immunization with a combination of a recombinant adhesin rAdr2 and a protein fragment rOmpB-4 derived from outer membrane protein B

Expression of Rickettsia Adr2 protein in E. coli is sufficient to promote resistance to complement-mediated killing, but not adherence to mammalian cells

Importancia de las proteínas OmpA y OmpB en el desarrollo de vacunas contra la rickettsiosis

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