Surface properties of sensors based on aminophenol-polymerized film

Abstract

In this work, carbon electrodes modified with aminophenols were developed for the production of pesticides biosensors based on acetylcholinesterase. The polymers were potentiodynamically deposited on a graphite electrode surface by the oxidation of monomers, 2-aminophenol, 3-aminophenol and 4-aminophenol. The electrochemical behaviour and surface analysis of the electrodes modified by polyaminophenols non-immobilized and immobilized on acetylcholinesterase were studied by cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy. Roughness values obtained for graphite electrodes modified with poly(4-aminophenol) and poly(4-aminophenol)/acetylcholinesterase were 174 and 86 nm, respectively. The acetylcholinesterase enzyme was immobilized on a graphite and a graphite modified with poly(4-aminophenol), and these electrodes were coupled in the flow system. Potentiometric response due to hydrogen ions generated by an enzymatic system in the presence of acetylcholine chloride substrate was evaluated. The results showed that the graphite/poly(4-aminhophenol) sensor presents high sensitivity to hydrogen ions when compared with other graphite/polyaminophenols sensors. The biosensor coupled in a continuous flow system was employed for the detection of dichlorvos. The detection and quantification limits were 0.8 and 2.4 μmol L −1 dichlorvos, respectively. This sensor reveals an efficient and promising material for biomolecules immobilization.

Detection of a Specific Biomarker for Epstein-Barr Virus Using a Polymer-Based Genosensor

“…Our research group has reported modifications of electrode surfaces with functionalized polymers, derived from aminophenols [ 28 – 31 ], tyramine [ 32 ], hydroxybenzoic acid [ 33 ], hidroxyphenylacetic acid [ 34 ] and methoxyphenethylamine [ 27 ], as well as application of the modified electrodes in the immobilization and detection of biomolecules [ 35 – 37 ].…”

Voltammetric Sensor Based on the Poly(p-aminobenzoic Acid) for the Simultaneous Quantification of Aromatic Aldehydes as Markers of Cognac and Brandy Quality

“…Electropolymerized coverages are widely studied modifiers of the electrode surface to date. Among various types of monomers, amino groups containing compounds (amino acids [ 27 , 28 , 29 , 30 ], aminophenols [ 31 , 32 , 33 , 34 ], aminobenzoic acids [ 35 , 36 , 37 ]) have been shown as effective modifiers of the electrode surface that provide significant improvement of the organic analytes response. Electrodes modified with MWCNTs and oxidized MWCNTs functionalized with 4-aminophenyl phosphonic acid [ 38 ] and aminobenzene acids [ 39 ] have been reported.…”

Desenvolvimento de imunossensor para detecção do hormônio tireoidiano L-triiodotironina (L-T3)

“…Os principais métodos de imobilização são: adsorção física, ligação covalente, imobilização por aprisionamento e imobilização por ligação cruzada 28 . Eletrodos modificados eletroquimicamente com filmes poliméricos que apresentam várias funções orgânicas, como por exemplo, poliaminofenóis 43,44 , poli(ácido hidroxifenilacético) 45 , politiramina 46,47 , poli(ácido hidroxibenzóico) 48 e poli(ácido fenilacético) 49 , possibilitam o uso de filmes catiônicos e aniônicos para a incorporação de biomoléculas e desenvolvimento de biossensores 43…”