Surface Properties of “Jellyfish”:  Langmuir Monolayer and Langmuir−Blodgett Film Studies of Recombinant Aequorin

Wang, Chengshan; Mićić, Miodrag; Ensor, Mark; Daunert, Sylvia; Leblanc, Roger M.

doi:10.1021/la700756e

Cited by 11 publications

(7 citation statements)

References 29 publications

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“…Materials. The synthesis and purification of the recombinant aequorin have been described in detail in a previous paper, and the protein is commercially available from MP Biomedicals LLC. (Irvine, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Higher potassium chloride concentration caused the increase of the limiting molecular area of the aequorin Langmuir monolayer. The addition of Ca 2+ into the subphase at pH 7.6 with KCl concentration at 0.5 M also caused a large increase of the limiting molecular area, from 4570 to 7180 Å 2 ·molecule -1 (as shown in Figure ), which was due to the transformation of aequorin to apoaequorin . However, the nature of this transformation and the reason why the transformation caused an increase of the limiting molecular area are not clear.…”

Section: Introductionmentioning

confidence: 97%

“…Consequently, it is important to study the surface properties of the recombinant aequorin. In our previous paper, the surface properties of the recombinant aequorin were studied in detail …”

Section: Introductionmentioning

confidence: 99%

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Infrared Reflection−Absorption Spectroscopy and Polarization-Modulated Infrared Reflection−Absorption Spectroscopy Studies of the Aequorin Langmuir Monolayer

et al. 2008

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The Langmuir monolayer of aequorin and apoaequorin was studied by infrared reflection-absorption spectroscopy (IRRAS) and polarization-modulated IRRAS techniques. The alpha-helices in the aequorin Langmuir monolayer were parallel to the air-water interface at zero surface pressure. When the surface pressure increased to 15 mN.(m-1), the alpha-helices became tilted and the turns became parallel to the air-water interface. As for apoaequorin, the alpha-helices were also parallel to the air-water interface at 0 mN.m(-1). However, the alpha-helix became tilted and the turns became parallel to the air-water interface quickly at 5 mN.m(-1). With further compression of the apoaequorin Langmuir monolayer, the orientation remained the same. The different behaviors of aequorin and apoaequorin at the air-water interface were explained by the fact that aequorin formed dimers at the air-water interface but apoaequorin was a monomer. It is more difficult for a dimer to be tilted by the compression of the Langmuir monolayer.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Infrared Reflection−Absorption Spectroscopy and Polarization-Modulated Infrared Reflection−Absorption Spectroscopy Studies of the Aequorin Langmuir Monolayer

et al. 2008

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“…To this aim, mean molecular area variation at a constant surface pressure was measured. 46,47 The films were ), K 2 CO 3 (0.67 equiv. ), KI (2.80 equiv.…”

Section: Studies At the Air-water Interfacementioning

confidence: 99%

“…The stability of the films formed with 1 and 2 was further checked in compression-expansion experiments. [46][47][48] As shown in Fig. 4, successive compression-expansion cycles of the calixarene films were performed from the gas phase to a mean molecular area around the more condensed state before collapse.…”

Section: Studies At the Air-water Interfacementioning

confidence: 99%

Organosoluble calixarene-based quinolone carriers: syntheses, evaluation and model hydrolytic studies at the air–water interface

Sautrey¹,

Clarot²,

Salem³

et al. 2012

New J. Chem.

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Two tetra-p-tert-butyl-and two tetra-p-H-calix [4]arene species integrating one or two propylnalidixate esters at the lower rim have been synthesized as possible antibacterial prodrugs. As they display amphiphilic behavior, their properties at the air-water interface were evaluated using the Langmuir balance technique. It has been shown that the two tert-butyl analogues form stable monolayers. The two tert-butyl analogues were then studied at 20 and 37 1C on carbonate buffered subphases at pH 10.0, before being engaged in hydrolytic studies on ''production'' of monolayers so as to allow a simple treatment, a HPLC survey of nalidixate release. The latter was found to be effective at 37 1C, with formation of free nalidixate in 13% yield for the bisnalidixate derivative, and 10% yield for the mononalidixate after 3 days.

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Mit Licht Tröpfchen in Bewegung setzen

Laloyaux

Jonas

2010

Angewandte Chemie

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[2] ist auch eine direkte Überführung von Licht in mechanische Arbeit möglich. Bei ihrer Untersuchung bewirkt die Lichtenergie eine lokale Veränderung des chemischen Potentials eines Tensids an der Oberfläche eines auf der Wasseroberfläche schwimmenden Öltropfchens, welche gemäß der Gibbs-Adsorptionsgleichung in eine lokale Veränderung der Oberflächenspannung übertragen wird (Abbildung 1).[3] Der resultierende Gradient der Oberflächenspannung ist das Oberflächen-¾quivalent des osmotischen Drucks; er führt zu Konvektionsströmen an der Oberfläche des Tröpfchens, die die Flüssigkeit von Bereichen mit geringerer Oberflächenspannung zu solchen mit höherer Oberflächenspannung bewegen. Ein Teil der Tröpf-chenoberfläche zieht daher, ähnlich einer elastischen Membran, an der im Tröpfchen enthaltenen Flüssigkeit, was insgesamt zu einer Verschiebung des Tröpfchens führt.

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Surface Properties of “Jellyfish”: Langmuir Monolayer and Langmuir−Blodgett Film Studies of Recombinant Aequorin

Cited by 11 publications

References 29 publications

Infrared Reflection−Absorption Spectroscopy and Polarization-Modulated Infrared Reflection−Absorption Spectroscopy Studies of the Aequorin Langmuir Monolayer

Infrared Reflection−Absorption Spectroscopy and Polarization-Modulated Infrared Reflection−Absorption Spectroscopy Studies of the Aequorin Langmuir Monolayer

Organosoluble calixarene-based quinolone carriers: syntheses, evaluation and model hydrolytic studies at the air–water interface

Mit Licht Tröpfchen in Bewegung setzen

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