Surface Properties of Bifidobacterial Strains of Human Origin

Pérez, Pablo; Minnaard, Yessica; Disalvo, E.A.; Antoni, Graciela L. De

doi:10.1128/aem.64.1.21-26.1998

Cited by 222 publications

(124 citation statements)

References 19 publications

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“…A relation between the aggregation and adhesion ability has been reported for some bifidobacterial species and Lactobacillus strains derived from the gut origin (Perez et al 1998;Del Re et al 2000;Kos et al 2003;Rickard et al 2003). In this study, a positive correlation between aggregation and internalization was found among Lact.…”

Section: Resultssupporting

confidence: 69%

An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity

Piwat

Sophatha

Teanpaisan

2015

Lett Appl Microbiol

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Adhesion to host tissues and bacterial aggregation (auto- and coaggregation) are the highly important criteria for selecting strains with probiotic potential. These abilities are commonly involved with surface-charged characteristics. This is the first study to investigate the oral Lactobacillus species using an oral keratinocyte cell line. Significant results were found for the correlations between the adhesion and surface charge characteristics and for aggregation among certain strains of Lactobacillus gasseri, Lactobacillus fermentum and Lactobacillus paracasei. This observation could be useful when collecting background information for the selection of probiotic strains for use in oral health.

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Section: Resultssupporting

confidence: 69%

An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity

Piwat

Sophatha

Teanpaisan

2015

Lett Appl Microbiol

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“…The hydrophobicity of a bacterial surface can been determined by direct methods, such as measuring the contact angle (CAM) (Cuperus et al 1993;Boonaert and Rouxhet 2000), and indirect method such as the MATH assay (Garrote et al 2004;Kathene et al 2007), which was used in this work. The assay was performed at pH 3 because at this pH hexadecane should not be charged, and any repulsive electrostatic forces are likely to be negligible (Busscher et al 1995;Perez et al 1998). Therefore, the interaction between bacterial cells and hexadecane was likely due to hydrophobic forces.…”

Section: Discussionmentioning

confidence: 99%

Effect of growth time on the surface and adhesion properties of Lactobacillus rhamnosus GG

Deepika

Green

Frazier

et al. 2009

Journal of Applied Microbiology

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Aims: To investigate the changes in the surface properties of Lactobacillus rhamnosus GG during growth, and relate them with the ability of the Lactobacillus cells to adhere to Caco‐2 cells. Methods and Results: Lactobacillus rhamnosus GG was grown in complex medium, and cell samples taken at four time points and freeze dried. Untreated and trypsin treated freeze dried samples were analysed for their composition using SDS‐PAGE analysis and Fourier transform infrared spectroscopy (FTIR), hydrophobicity and zeta potential, and for their ability to adhere to Caco‐2 cells. The results suggested that in the case of early exponential phase samples (4 and 8 h), the net surface properties, i.e. hydrophobicity and charge, were determined to a large extent by anionic hydrophilic components, whereas in the case of stationary phase samples (13 and 26 h), hydrophobic proteins seemed to play the biggest role. Considerable differences were also observed between the ability of the different samples to adhere to Caco‐2 cells; maximum adhesion was observed for the early stationary phase sample (13 h). The results suggested that the adhesion to Caco‐2 cells was influenced by both proteins and non‐proteinaceous compounds present on the surface of the Lactobacillus cells. Conclusion: The surface properties of Lact. rhamnosus GG changed during growth, which in return affected the ability of the Lactobacillus cells to adhere to Caco‐2 cells. Significance and Impact of the Study: The levels of adhesion of Lactobacillus cells to Caco‐2 cells were influenced by the growth time and reflected changes on the bacterial surface. This study provides critical information on the physicochemical factors that influence bacterial adhesion to intestinal cells.

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“…Several works have investigated the composition, structure and forces of interaction related to bacterial adhesion to intestinal epithelial cells (Perez et al 1998;Del et al 2000). In most cases, aggregation ability is related to cell adherence properties (Boris et al 1997;Del et al 2000).…”

Section: Discussionmentioning

confidence: 99%

In vitroevaluation on adherence and antimicrobial properties of a candidate probioticClostridium butyricumCB2 for farmed fish

Pan

Zhang

et al. 2008

Journal of Applied Microbiology

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Aims: To characterize the antimicrobial and adhesion ability of candidate probiotic Clostridium butyricum CB2 for farmed fish in vitro. Methods and Results: The potential probiotic Cl. butyricum CB2 had been evaluated for its adhesion capacity and antagonistic effect against two fish pathogens Aeromonas hydrophila and Vibrio anguillarum by the intestinal cell model. In addition, the aggregation ability and antimicrobial property on agar plate were assayed. The results indicated that the candidate probiotic Cl. butyricum CB2 have strong adhesion property and a higher antagonistic activity to Aer. hydrophila and V. anguillarum both on agar plate and cell model. Clostridium butyricum showed a higher aggregation which might be the reasons for bacteria adhesion and antimicrobial activity. Conclusions: The strain Cl. butyricum CB2 could be used as potential probiotic to inhibit pathogens growth and prevent their colonization in fish intestinal tract. Significance and Impact of the Study: This study revealed the antimicrobial and adhesion characteristic of Cl. butyricum CB2 which was selected as the potential probiotic to farmed fish.

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Surface Properties of Bifidobacterial Strains of Human Origin

Cited by 222 publications

References 19 publications

An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity

An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity

Effect of growth time on the surface and adhesion properties of Lactobacillus rhamnosus GG

In vitroevaluation on adherence and antimicrobial properties of a candidate probioticClostridium butyricumCB2 for farmed fish

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