Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips

Mercey, Emilie; Grosjean, Ludivine; Roget, André; Livache, Thierry

doi:10.1007/978-1-59745-426-1_12

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…Until now electrospotting as a mean for microarray fabrication was used only for immobilization, i.e., to immobilize pyrrole labeled biomolecules (oligonucleotides, peptides, proteins and oligosaccharides) (Mercey et al, 2007;Villiers et al, 2009) onto SPRi chips with higher stability than classical self-assembled monolayer-based immobilization methods (Guedon et al, 2000). The biomolecules were coupled through a very thin polymeric layers of less than 10 nm to enable properly sensitive SPR monitoring of target binding to the attached biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Bosserdt

Erdőssy²,

Lautner

et al. 2015

Biosensors and Bioelectronics

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Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500 µm diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application.

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Section: Introductionmentioning

confidence: 99%

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Bosserdt

Erdőssy²,

Lautner

et al. 2015

Biosensors and Bioelectronics

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Recent Trends in Polypyrrole Electrochemistry, Nanostructuration, and Applications

Audebert

2010

Electropolymerization

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IntroductionThis chapter covers recent original research on polypyrrole, especially that pertaining to the last four or five years. It focuses on the synthesis and electrochemical properties of polypyrrole and polypyrrole derivatives, at the same time describing some interesting chemical syntheses and related composites. Physicochemical properties and related applications are mainly discussed, with a brief mention of the field of related biosensors and bioactive polymers. In fact, a more detailed discussion on these topics would make this article too long; moreover, there are several chapters in this book that are dedicated to this area.Since its original discovery by Diaz et al. [1a], polypyrrole progressively tends to become an old polymer. Meanwhile, the trend of the whole polypyrrole related research field follows its way, including many more technical progresses and focusing more on precise applications. However, it is possible to distinguish major and minor fields within this area. While research on new monomers has slowed down, investigations of new polymerization methods, both chemically and electrochemically, are still being carried out. The field of electrochemistry, for example, has seen a notable breakthrough in the use of ionic liquids for electrochemical synthesis, and there is a clear increase in research in the direction of syntheses of all kinds of prestructured media such as templates, emulsions, gels, nanoparticles arrays, membranes, and so on. After the initial excitement on research in the field of accumulators, enthusiasm in this area of application has dwindled; however, related applications such as supercapacitors continue to stimulate a steady amount of research work. Furthermore, the main focus of scientists is now on applications, especially on the design of polypyrrole derivatives fitted to specific purposes.Interesting reviews covering this field have appeared in recent years [1b,c]. These will be referred to in the chapter.This chapter is divided into three parts. The first part discusses the advances in polypyrrole synthesis, including new original monomers that appeared in the last five years, new polymerization procedures, and composites; a few recent fundamental studies on redox processes in polypyrrole are also mentioned. The Electropolymerization: Concepts, Materials and Applications. Edited by Serge Cosnier and Arkady Karyakin

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Survey of the year 2007 commercial optical biosensor literature

Rich

Myszka

2008

J of Molecular Recognition

163

121

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In 2007, 1179 papers were published that involved the application of optical biosensors. Reported developments in instrument hardware, assay design, and immobilization chemistry continue to improve the technology's throughput, sensitivity, and utility. Compared to recent years, the widest range of platforms, both traditional format and array-based, were used. However, as in the past, we found a disappointingly low percentage of well-executed experiments and thoughtful data interpretation. We are alarmed by the high frequency of suboptimal data and over-interpreted results in the literature. Fortunately, learning to visually recognize good--and more importantly, bad--data is easy. Using examples from the literature, we outline several features of biosensor responses that indicate experimental artifacts versus actual binding events. Our goal is to have everyone, from benchtop scientists to project managers and manuscript reviewers, become astute judges of biosensor results using nothing more than their eyes.

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Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips

Cited by 4 publications

References 21 publications

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

Recent Trends in Polypyrrole Electrochemistry, Nanostructuration, and Applications

Survey of the year 2007 commercial optical biosensor literature

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