Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

“…An aldehyde function at the surface of glass slides in combination with amino-oxy-acetyl moieties in the peptides [15,[26][27][28][29][30] or cysteinyl-residues [26,[31][32][33][34] was used for the preparation of peptide microarrays on glass slides. The reaction between cysteine residues and surface-bound maleimide groups was used for preparation of peptide microarrays for kinase [35][36][37][38][39][40] and protease profiling [41].…”

Section: Chemistry Of Peptide Array Preparationmentioning

confidence: 99%

Deciphering Enzyme Function Using Peptide Arrays

2011

View full text Add to dashboard Cite

Enzymes are key molecules in signal-transduction pathways. However, only a small fraction of more than 500 human kinases, 300 human proteases and 200 human phosphatases is characterised so far. Peptide microarray based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last years. This technology reduces set-up time for HTS assays and allows the identification of downstream targets. Moreover, peptide microarrays enable optimisation of enzyme substrates. Focus of this review is on assay principles for measuring activities of kinases, phosphatases or proteases and on substrate identification/optimisation for kinases. Additionally, several examples for reliable identification of substrates for lysine methyl-transferases, histone deacetylases and SUMO-transferases are given. Finally, use of high-density peptide microarrays for the simultaneous profiling of kinase activities in complex biological samples like cell lysates or lysates of complete organisms is described. All published examples of peptide arrays used for enzyme profiling are summarised comprehensively.

show abstract

“…This way, lysates can be analyzed for their caspase activity, which provides valuable information for cell-based drug screening [22]. …”

Section: Different Formats Of Microarraysmentioning

confidence: 99%

Facing Current Quantification Challenges in Protein Microarrays

Wellhausen

Seitz

2012

Journal of Biomedicine and Biotechnology

View full text Add to dashboard Cite

The proteome is highly variable and differs from cell to cell. The reasons are posttranslational modifications, splice variants, and polymorphisms. Techniques like next-generation sequencing can only give an inadequate picture of the protein status of a cell. Protein microarrays are able to track these changes on the level they occur: the proteomic level. Therefore, protein microarrays are powerful tools for relative protein quantification, to unveil new interaction partners and to track posttranslational modifications. This papers gives an overview on current protein microarray techniques and discusses recent advances in relative protein quantification.

show abstract

Surface plasmon resonance imaging measurements of caspase reactions on peptide microarrays

Cited by 21 publications

References 8 publications

Peptide Arrays: Development and Application

Peptide Arrays: Development and Application

Deciphering Enzyme Function Using Peptide Arrays

Facing Current Quantification Challenges in Protein Microarrays

Contact Info

Product

Resources

About