2011
DOI: 10.1098/rsif.2010.0747
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Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin

Abstract: The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV-visible absorption spectroscopy. A value of 1.3 Â 10 7 M 21 was obtained for the… Show more

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Cited by 36 publications
(23 citation statements)
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References 38 publications
(49 reference statements)
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“…A high surface coverage is seen for the PU-Ag nanoparticles ( Figure 2 e); solvent exposure was likely to have led to sub-surface particle penetration. Ag nanoparticles (AgNPs) of increased size ranging from 30 to 290 nm were coated on the PU-AgNPs composite film compared to the original 30–40 nm [ 40 ].…”
Section: Resultsmentioning
confidence: 99%
“…A high surface coverage is seen for the PU-Ag nanoparticles ( Figure 2 e); solvent exposure was likely to have led to sub-surface particle penetration. Ag nanoparticles (AgNPs) of increased size ranging from 30 to 290 nm were coated on the PU-AgNPs composite film compared to the original 30–40 nm [ 40 ].…”
Section: Resultsmentioning
confidence: 99%
“…Ideally, the physico-chemical properties of the capsule allow for preferential interaction with a specific type of cell [ 5 , 7 ]. Alternatively, magnetic nanoparticles that accumulate in certain tissues can be used to detect and image disease [ 2 , 9 ]. However, significant questions remain regarding how altering the physico-chemical properties of nanoparticles during synthesis influences the cellular internalization of the nanoparticle, and hence influences the efficacy of the putative therapy [ 10 ].…”
Section: Introductionmentioning
confidence: 99%
“…At the same time, along with the fabrication of Ag nanostructures, a peak was observed at $380 nm near the bandgap, which can be due to the quadrupolar resonance of Ag nanostructures [39] and it was constantly observed in the reflectance spectra without a shift. Also, an absorption valley was observed at $450 nm along with the strong reflectance shoulder over the VIS-NIR region [40]. The absorption valley was nearly constant at $450 between 150 and 300 8C due to the similar morphology and gradually red shifted to $495 nm between 350 and 650 8C likely due to the absorption band shift by the increased size of Ag nanostructures [39].…”
Section: Originalmentioning
confidence: 91%