Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Chammem, Hanen; Hafaid, Imen; Meilhac, Olivier; Menaa, Farid; Mora, Laurence; Abdelghani, Adnane

doi:10.4236/jbnb.2014.53018

Cited by 18 publications

(9 citation statements)

References 13 publications

(13 reference statements)

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“…Mainly, physisorption does not depend on multistep and long experimental procedures (Marrakchi et al, ). In addition, physisorbed phages have been described to promote bacteria‐specific capture, infection, and lysis, when monitored by SPR (Chammem et al, ; Mejri et al, ). Physisorption was used in this work for transducer functionalization based on its simplicity.…”

Section: Resultsmentioning

confidence: 99%

Pathogenic Escherichia coli biosensor detection on chicken food samples

Helali

Alatawi

Abdelghani

2018

Journal of Food Safety

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Escherichia coli is an important pollution indicator and is the most important foodborne pathogens of public health concern. The chicken meat is one of the most important sources of good quality protein, and it is also susceptible to microbial contamination and often implicated in foodborne disease. In such context, the detection of E. coli K12 in frozen chicken meat was investigated by electrochemical impedance spectroscopy and surface plasmon resonance imaging techniques. The anti‐E. coli antibody was first immobilized onto gold surface by physisorption technique. The electrical and optical properties of the immobilized anti‐E. coli K12 antibody were studied. The binding of the E. coli K12 bacteria with the anti‐E. coli antibody layer was measured with a detection limit of 103 cfu/ml. This detection limit is better than those obtained with the enzyme‐linked immunosorbent assay (ELISA) technique. Moreover, the developed biosensor was used for E. coli K12 detection in inoculated frozen chicken meat. Practical applications Escherichia coli is an important pollution indicator and is the most important foodborne pathogens of public health concern. The present study deals with the development of bacteria biosensors based on electrochemical impedance spectroscopy and surface plasmon resonance imaging techniques for E. coli K12 detection in inoculated frozen chicken meat. A detection limit of 103 cfu/ml was obtained with the developed biosensors that was better than those obtained with classical methods such as ELISA (enzyme‐linked immunosorbent assay). This research work opens tremendous potential applications for the detection of pathogens in food at the early stage particularly with the development of miniaturized multiarrays platform based on impedance spectroscopy.

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Section: Resultsmentioning

confidence: 99%

Pathogenic Escherichia coli biosensor detection on chicken food samples

Helali

Alatawi

Abdelghani

2018

Journal of Food Safety

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“…The paper-based discs are disposable while the PCB substrate can be reused. In comparison to other rapid, sensitive and portable label-free biosensors for protein quantification that have recently been reported in the literature [16][17][18][19][20][21][22][23][24][25][26][27][28], the presented method has improved cost-effectiveness, simplification and ease of use. Thus, although an increased upper limit of detection for our presented methodology is suspected, further research should be carried out to determine whether it may be applicable for the measurement of protein biomarkers of a broadened range of concentrations.…”

Section: Analysis and Comparison With Elisa And Recently Developed Mementioning

confidence: 97%

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

et al. 2019

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There is a growing need for biosensors that are capable of efficiently and rapidly quantifying protein biomarkers, both in the biological research and clinical setting. While accurate methods for protein quantification exist, the current assays involve sophisticated techniques, take long to administer and often require highly trained personnel for execution and analysis. Herein, we explore the development of a label-free biosensor for the detection and quantification of a standard protein. The developed biosensors comprise carbon nanotubes (CNTs), a specific antibody and cellulose filtration paper. The change in electrical resistance of the CNT-based biosensor system was used to sense a standard protein, bovine serum albumin (BSA) as a proof-of-concept. The developed biosensors were found to have a limit of detection of 2.89 ng/mL, which is comparable to the performance of the typical ELISA method for BSA quantification. Additionally, the newly developed method takes no longer than 10 min to perform, greatly reducing the time of analysis compared to the traditional ELISA technique. Overall, we present a versatile, affordable, simplified and rapid biosensor device capable of providing great benefit to both biological research and clinical diagnostics.

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“…Mainly, physisorption does not depend on multistep, long experimental procedures and is easily reversed [19]. In addition, physisorbed phages have been described to promote bacteria-specific capture, infection, and lysis, when monitored by SPR [20,21]. This work was carried using physisorption functionalization based on its simplicity.…”

Section: Physisorption Of Polyclonal Antibody On Interdigitated Gold mentioning

confidence: 99%

Fast Detection of Pathogenic Escherichia coli from Chicken Meats

Helali¹,

Abdelghani²

2020

E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment

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Food is a means to sustain and enjoy life, but it is also a medium for microbial contamination, causing disease and death. Fruits, vegetables, meat, and water are the common sources of contamination. Escherichia coli is one of the most frequent Pathogenic Bacteria responsible for food poisoning and food-related infections. E. coli infection causes severe bloody diarrhea, abdominal cramps, and occasional vomiting. In the present study, electrochemical impedance spectroscopy (EIS), surface plasmon resonance (SPR), and physisorption techniques were evaluated to decrease sample preparation time and to improve the sensitivity and specificity for the detection of low levels of pathogenic Escherichia coli in frozen chicken meat. The electrical and optical properties of the immobilized anti-E. coli antibody were studied. Moreover, the developed biosensor was used for E. coli detection in inoculated frozen chicken meat.

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Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma

Cited by 18 publications

References 13 publications

Pathogenic Escherichia coli biosensor detection on chicken food samples

Pathogenic Escherichia coli biosensor detection on chicken food samples

Carbon Nanotube-Based Electrochemical Biosensor for Label-Free Protein Detection

Fast Detection of Pathogenic Escherichia coli from Chicken Meats

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