Surface plasmon resonance biosensor for the detection of miRNAs by combining the advantages of homogeneous reaction and heterogeneous detection

Huang, Yaliang; Sun, Ting; Liu, Lin; Xia, Ning; Zhao, Yuehua; Yi, Xinyao

doi:10.1016/j.talanta.2021.122622

Cited by 16 publications

(11 citation statements)

References 61 publications

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“…Huang et al developed a sensor to detect miRNA (micro-RNA) using AuNPs to enhance the SPR signal [151]. The SPR chip surface was modified with streptavidin (SA), while DNA was modified with biotin (bio) at the beginning and the end of the filament (bio-DNA-bio).…”

Section: Biosensorsmentioning

confidence: 99%

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Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

et al. 2021

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Gold and Silver nanoparticles (AuNPs and AgNPs) are perfect platforms for developing sensing colorimetric devices thanks to their high surface to volume ratio and distinctive optical properties, particularly sensitive to changes in the surrounding environment. These characteristics ensure high sensitivity in colorimetric devices. Au and Ag nanoparticles can be capped with suitable molecules that can act as specific analyte receptors, so highly selective sensors can be obtained. This review aims to highlight the principal strategies developed during the last decade concerning the preparation of Au and Ag nanoparticle-based colorimetric sensors, with particular attention to environmental and health monitoring applications.

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Section: Biosensorsmentioning

confidence: 99%

“…The bio-DNA can react with the surface of the SPR chip but not with SA-AuNPs. Since the SA-AuNPs are not bound to the DNA, the sensor signal decreases proportionally to the miRNA concentration [151].…”

Section: Biosensorsmentioning

confidence: 99%

Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

et al. 2021

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“…SA is a tetrameric protein that can bind to four biotin molecules with high binding affinity in a wide pH range (K d = 10 −15 M) [82,83]. The specific and strong interaction is always utilized for the conjugation of antibodies or nucleic acids with enzymes or nanomaterials for signal output.…”

Section: Sa-biotin Interactionmentioning

confidence: 99%

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

et al. 2021

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The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design of electrochemical biosensors for the signal output and amplification. In this review, we highlight the significant progress in the in situ assembly of nanomaterials as the nanolabels for enhancing the performances of electrochemical biosensors. The works are discussed based on the difference in the interactions for the assembly of nanomaterials, including DNA hybridization, metal ion–ligand coordination, metal–thiol and boronate ester interactions, aptamer–target binding, electrostatic attraction, and streptavidin (SA)–biotin conjugate. We further expand the range of the assembly units from nanomaterials to small organic molecules and biomolecules, which endow the signal-amplified strategies with more potential applications.

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“…However, traditional SPR sensors are less sensitive for the direct detection of small molecules (<8 kDa) or ultra-low concentrations (1 pM) of analytes based on the change in refractive index or thickness near metal surfaces [ 1 , 2 ]. In view of this, many efforts have been made to improve the sensitivity of SPR biosensors using secondary signal amplification with gold nanomaterials, magnetic nanoparticles and biomacromolecules [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The targets can be determined with a competitive (signal-off) or sandwich-like (signal-on) format.…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance Biosensors with Magnetic Sandwich Hybrids for Signal Amplification

Sun

Zhao

et al. 2022

Biosensors

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The conventional signal amplification strategies for surface plasmon resonance (SPR) biosensors involve the immobilization of receptors, the capture of target analytes and their recognition by signal reporters. Such strategies work at the expense of simplicity, rapidity and real-time measurement of SPR biosensors. Herein, we proposed a one-step, real-time method for the design of SPR biosensors by integrating magnetic preconcentration and separation. The target analytes were captured by the receptor-modified magnetic nanoparticles (MNPs), and then the biotinylated recognition elements were attached to the analyte-bound MNPs to form a sandwich structure. The sandwich hybrids were directly delivered to the neutravidin-modified SPR fluidic channel. The MNPs hybrids were captured by the chip through the neutravidin–biotin interaction, resulting in an enhanced SPR signal. Two SPR biosensors have been constructed for the detection of target DNA and beta-amyloid peptides with high sensitivity and selectivity. This work, integrating the advantages of one-step, real-time detection, multiple signal amplification and magnetic preconcentration, should be valuable for the detection of small molecules and ultra-low concentrations of analytes.

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Surface plasmon resonance biosensor for the detection of miRNAs by combining the advantages of homogeneous reaction and heterogeneous detection

Cited by 16 publications

References 61 publications

Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

Gold and Silver Nanoparticle-Based Colorimetric Sensors: New Trends and Applications

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

Surface Plasmon Resonance Biosensors with Magnetic Sandwich Hybrids for Signal Amplification

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