Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection

Abstract

In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.

Testosterone Imprinted poly(HEMA‐MAA) Nanoparticles Based Surface Plasmon Resonance Sensor for Detection of Testosterone

“…m is the slope of the regression line and S is the standard deviation of the intercept. [22] The LOD value was recorded as 0.049 ng/mL for testosterone imprinted SPR sensor. Table 1 summarizes several of the different sensor studies for testosterone detection in the literature.…”

“…Molecularly imprinted polymers (MIPs) are polymeric structures prepared by polymerizing a functional monomer with a crosslinker in the presence of a target molecule that acts as a molecular template. [21,22] After the target molecule is removed with a suitable desorption agent, three-dimensional specific cavities belonging to that target molecule are formed in the structure. Molecularly imprinted polymers have important properties such as being inexpensive, easy to prepare, and long-term stability, resistant to harsher environmental conditions such as organic solvent, high pH and temperatures.…”

Real‐Time Detection of Fibrinogen via Imprinted Recognition Sites

“…Therefore, the linear equation of the fibrinogen imprinted biosensor between 0.05-3.0 mg/mL concentrations was created as y = 6.7367x-0.5871 with R 2 value of 0.9919. The limit of detection (LOD = 3.3S/m) and quantification (LOQ = 10S/m) of fibrinogen molecules was calculated based on the slope of the calibration curve [24] LOD and LOQ values were calculated as 0.00066 and 0.00219 mg/mL, respectively. Other sensor studies performed for fibrinogen determination are given in Table 1 for comparison.…”

“…[20][21][22] Furthermore, the ability to perform simultaneous assays rapidly allows the use of the instrument for point-of-care applications where multiple parameters are desired to be monitored. [23][24][25] Consequently, the integration of MIMs with the SPR system has been made excellent improvements in sensing technology. [26][27][28][29] In this study, the designed SPR sensing platform was based on the integration of molecular imprinting with SPR system for fibrinogen detection patient serum samples.…”

A Novel Dual-Wavelength Method for Evaluating Temperature Effect in Fiber-Optic SPR Sensors

“…Therefore, a fiberoptic sensor using the SPR (also called fiber-optic SPR sensor) can be designed with different molecular sensor layers on the metal surface. The fiber-optic SPR sensor is a novel bio-chemical sensor that can be applied broadly in the fields of biomolecular interaction analysis, chemical/biological analytes detection and medical diagnostics [5][6][7][8]. It is capable of detecting tiny RI changes of an analyte or other physical quantities that equivalently convert to RI changes [9,10].…”