Surface plasmon resonance-based detection of ladder-shaped polyethers by inhibition detection method

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

“…Surface plasmon resonance and resonant mirror biosensors have been used for its detection based on its interaction with phosphodiesterase enzymes, using either direct format or competition format assays [69][70][71][72]. The interaction with phosphodiesterase allows the detection of yessotoxin in the low µM range, enough for current regulatory limits, however the method is not as specific as immuno-based biosensors, since other compounds such as the brevetoxin PbTx1 and other polyethers can also interact with phosphodiesterases [72].…”

“…Surface plasmon resonance and resonant mirror biosensors have been used for its detection based on its interaction with phosphodiesterase enzymes, using either direct format or competition format assays [69][70][71][72]. The interaction with phosphodiesterase allows the detection of yessotoxin in the low µM range, enough for current regulatory limits, however the method is not as specific as immuno-based biosensors, since other compounds such as the brevetoxin PbTx1 and other polyethers can also interact with phosphodiesterases [72]. Other methods with biological components include a microplate assay based on the activation of phosphodiesterase enzymatic activity by yessotoxins [73], which was the basis for the development of these biosensor methods, and a direct assay that detects the interaction of these toxins with phosphodiesterases by fluorescence polarization [74].…”

Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins

“…Surface plasmon resonance and resonant mirror biosensors have been used for its detection based on its interaction with phosphodiesterase enzymes, using either direct format or competition format assays [69-72]. The interaction with phosphodiesterase allows the detection of yessotoxin in the low μM range, enough for current regulatory limits, however the method is not as specific as immuno-based biosensors, since other compounds such as the brevetoxin PbTx1 and other polyethers can also interact with phosphodiesterases [72].…”

“…Surface plasmon resonance and resonant mirror biosensors have been used for its detection based on its interaction with phosphodiesterase enzymes, using either direct format or competition format assays [69-72]. The interaction with phosphodiesterase allows the detection of yessotoxin in the low μM range, enough for current regulatory limits, however the method is not as specific as immuno-based biosensors, since other compounds such as the brevetoxin PbTx1 and other polyethers can also interact with phosphodiesterases [72]. Other methods with biological components include a microplate assay based on the activation of phosphodiesterase enzymatic activity by yessotoxins [73], which was the basis for the development of these biosensor methods, and a direct assay that detects the interaction of these toxins with phosphodiesterases by fluorescence polarization [74].…”

“…In the biosensors field, a surface plasmon resonance-based detection method for ladder-shaped polyeher compounds (among them brevetoxin-2) has been recently published [72]. The ability of these molecules to inhibit the interaction of desulfo-yessotoxin to phosphodiesterase II was used to design an indirect assay format that can detect several toxins.…”