Surface-plasmon opto-electrochemistry

“…Last years, a combination of surface plasmon resonance reflectometry (SPR) with electrochemical methods was suggested [7][8][9][10][11][12][13][14]. In this paper we use this combination to study mercury reduction on gold surface.…”

Section: Introductionmentioning

confidence: 99%

SPR investigation of mercury reduction and oxidation on thin gold electrodes

Vasjari

Shirshov

Samoylov

et al. 2007

Journal of Electroanalytical Chemistry

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“…This so-called electrochemical SPR or E-SPR phenomenon depends on the ionic composition of the electrolyte and on the electron concentration in the metal. As described in the literature [34], to a first approximation, the refractive index of the double layer can be considered to vary with the change in the charge of the double layer. Lioubimov et al [35] described a combination of oscillating electric potential and SPR measurement.…”

Section: Pumping Liquids Using Electroosmotic Flow In Microfluidic Dementioning

confidence: 99%

Future Trends in SPR Technology

Schasfoort

Schuck

2008

Handbook of Surface Plasmon Resonance

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Krone et al. [3] first combined SPR and mass spectrometry (MS), which created a unique approach for protein investigations. This technique was adapted and modified in several laboratories [4][5][6][7] and some of the commercial instruments are now also equipped with basic analyte recovery capability for MS. The basic idea is to follow up the characterization of interactions between proteins and surface-immobilized ligands by SPR with the determination of the identity of the bound proteins or peptides using MS. This has applications in protein interaction discovery in proteomics, and also the characterization of protein modifications critical for the interaction. Considering the SPR sensor surface as a miniaturized chromatographic matrix, SPR-MS is reminiscent also of the traditional affinity chromatographic purification preceding MS in protein discovery, but obviously with added real-time quantification of the capturing and elution process, in addition to exhibiting different elution behavior due to the much smaller scale matrix. Several fundamentally different interface approaches have been developed by different groups, for example (1) direct use of the SPR chip surface for MS by mounting the preloaded SPR chip on a MALDI platform [3] or analyzing the surface with a SELDI protein chip reader [7]; (2) on-chip digestion followed by microfluidic elution, recovery in a reversed-phase microcapillary column and ESI-MS/MS sequencing of peptides from the digest [5]; and (3) analyte dissociation, microfluidic elution and collection followed by external digestion and MALDI-TOF sequencing [4,9]. Recent progress in SPR-MS includes improved methods and operations, increased limits of detection, multi-protein analysis and protein-complex delineation. With the subsequent design of SPR protein arrays, SPR-MS enters into the field of high-throughput protein interaction discovery and miniaturized diagnostics.Below we briefly highlight an example of SPR detection of an enrichment and elution process of specifically bound analyte as an application of SPR-MS by Visser et al. (Heck's group) at the University of Utrecht (The Netherlands) [8]. In this work, an SPR-based chemical proteomics approach in combination with nano-liquid chromatography/electrospray tandem mass spectrometry (nanoLC/ESI-MS/MS) was used to obtain both semi-quantitative and qualitative data on enriched proteins obtained from SPR sensor surfaces. The aim of the research was the characterization and identification of autoimmune antibody-antigen complexes present in RA patient sera. The autoimmune antibodies can be enriched using a citrullinated peptide (i.e. a specific peptide where the arginine is replaced by citrulline) as a ligand which is immobilized on a geltype G-COOH sp IBIS sensor chip. The work is connected to the research described in Chapter 7, which should be consulted for details and references. 357 Future Trends in SPR Technology

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Surface-plasmon opto-electrochemistry

Cited by 18 publications

References 17 publications

Surface-plasmon voltammetry using a gold grating

Surface-plasmon voltammetry using a gold grating

SPR investigation of mercury reduction and oxidation on thin gold electrodes

Future Trends in SPR Technology

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