Surface plasmon-coupled emission (SPCE)-based immunoassay using a novel paraboloid array biochip

Yuk, Jong Seol; Trnavsky, Michal; McDonagh, Colette; MacCraith, Brian D.

doi:10.1016/j.bios.2009.10.026

Cited by 44 publications

(43 citation statements)

References 35 publications

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“…5-9 These attractive characteristics of SPCE have high implications in sensing applications and expected to bring increased interest on the SPCE based imaging methodologies. 10-13 …”

Section: Introductionmentioning

confidence: 99%

Metal–Dielectric Waveguides for High Efficiency Fluorescence Imaging

et al. 2015

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We demonstrate that Metal–Dielectric Waveguide structures (MDWs) with high efficiency of fluorescence coupling can be suitable as substrates for fluorescence imaging. This hybrid MDWs consists of a continuous metal film and a dielectric top layer. The optical modes sustaining inside this structure can be excited with a high numerical aperture (N.A) objective, and then focused into a virtual optical probe with high intensity, leading to efficient excitation of fluorophores deposited on top of the MDWs. The emitted fluorophores couple with the optical modes thus enabling the directional emission, which is verified by the back focal plane (BFP) imaging. These unique properties of MDWs have been adopted in a scanning laser confocal optical microscopy, and show the merit of high efficiency fluorescence imaging. MDWs can be easily fabricated by vapor deposition and/or spin coating, the silica surface of the MDWs is suitable for biomolecule tethering, and will offer new opportunities for cell biology and biophysics research.

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“…5-9 These attractive characteristics of SPCE have high implications in sensing applications and expected to bring increased interest on the SPCE based imaging methodologies. 10-13 …”

Section: Introductionmentioning

confidence: 99%

Metal–Dielectric Waveguides for High Efficiency Fluorescence Imaging

et al. 2015

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“…This is an important factor in designing highly sensitive assay platforms. Some SAF strategies used in the past by the authors and others include the use of a parabolic glass or polymer elements to collect the SAF light and re-direct to the detector [5,6] and arraybased collection elements [3]. A disadvantage of the paraboloid approach reported previously [5] is that optical contact between the chip and the collection optics has to be made using refractive index matching oils or gels which is inconvenient for point-of-care applications and adds to the complexity of the design.…”

Section: Introductionmentioning

confidence: 99%

“…This light constitutes the Supercritical Angle Fluorescence or SAF which is not captured in conventional detection systems which are also often characterized by low numerical aperture optics. This light can be harnessed by a suitable re-design of the solid substrate [3]. As well as enhanced light collection, another key advantage of SAF for bioassay applications is that SAF detection allows collection of fluorescence only from molecules adjacent to the chip surface, hence minimizing background fluorescence from the bulk solution [4].…”

Section: Introductionmentioning

confidence: 99%

High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance

Kurzbuch¹,

Somers²,

McDonagh³

2013

Opt. Express

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We present a polymer biochip with embedded optics which allows the detection of supercritical angle fluorescence (SAF) without losses due to total internal reflection within the substrate. The chip design comprises structured spherical and aspherical optical elements on the bottom, while the top is chemically functionalized for direct binding of biomolecules. Furthermore, this design facilitates integration in lab-on-a-chip systems with appropriate microfluidics. In the confocal optical setup an ellipsoidal mirror is used for collection of SAF light above the critical angle of the water-polymer interface, which is detected by a photon-counting detector. The work presented here represents a proof of concept for performing sensitive and rapid point-of-care testing, using this low-cost, robust and disposable optical biochip platform. The performance of the platform was validated using direct binding DNA and human IgG assays which yielded low limits of detection 10 pM for DNA and 10 pg/ml for human IgG.

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“…2–4 The use of this phenomenon for better fluorescence detection, immunoassays, bio-sensing and background reduction has been widely investigated. 5–9 Despite these improvements, SPCE has a limitation because the emission occurs at angles larger than the critical angle of the glass/air interface. The appearance of the emitted light at large angles relative to the surface normal makes it difficult to collect the coupled emission and is unsuitable for high throughput or array-based applications.…”

Section: Introductionmentioning

confidence: 99%

Directional Emission from Metal–Dielectric–Metal Structures: Effect of Mixed Metal Layers, Dye Location, and Dielectric Thickness

et al. 2015

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Metal-dielectric-metal (MDM) structures provide directional emission close to the surface normal, which offers opportunities for new design formats in fluorescence based applications. The directional emission arises due to near-field coupling of fluorophores with the optical modes present in the MDM substrate. Reflectivity simulations and dispersion diagrams provide a basic understanding of the mode profiles and the factors that affect the coupling efficiency and the spatial distribution of the coupled emission. This work reveals that the composition of the metal layers, the location of the dye in the MDM substrate and the dielectric thickness are important parameters that can be chosen to tune the color of the emission wavelength, the angle of observation, the angular divergence of the emission and the polarization of the emitted light. These features are valuable for displays and optical signage.

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Surface plasmon-coupled emission (SPCE)-based immunoassay using a novel paraboloid array biochip

Cited by 44 publications

References 35 publications

Metal–Dielectric Waveguides for High Efficiency Fluorescence Imaging

Metal–Dielectric Waveguides for High Efficiency Fluorescence Imaging

High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance

Directional Emission from Metal–Dielectric–Metal Structures: Effect of Mixed Metal Layers, Dye Location, and Dielectric Thickness

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