Surface plasma functionalization influences macrophage behavior on carbon nanowalls

Ion, Raluca; Vizireanu, Sorin; Stancu, Claudia Elena; Luculescu, C.; Cîmpean, Anișoara; Dinescu, Gheorghe

doi:10.1016/j.msec.2014.11.064

Cited by 32 publications

(25 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This confirmed that the cells could detect the topographical features that the CNW surface presented to them. As was previously observed with similar CNW structures, adherent cells can be encouraged to migrate and proliferate, or be inhibited to adhere, depending on the cell type and functionalization of the CNW edge [ 51 , 52 , 54 , 55 ]. The adhesion mechanism proposes an extension-latch-and-release process, similar to the observations made in a lower density CNWs like the ones produced in this study [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation

et al. 2018

View full text Add to dashboard Cite

Nanostructured graphene films were used as platforms for the differentiation of Saos-2 cells into bone-like cells. The films were grown using the plasma-enhanced chemical vapor deposition method, which allowed the production of both vertically and horizontally aligned carbon nanowalls (CNWs). Modifications of the technique allowed control of the density of the CNWs and their orientation after the transfer process. The influence of two different topographies on cell attachment, proliferation, and differentiation was investigated. First, the transferred graphene surfaces were shown to be noncytotoxic and were able to support cell adhesion and growth for over 7 days. Second, early cell differentiation (identified by cellular alkaline phosphatase release) was found to be enhanced on the horizontally aligned CNW surfaces, whereas mineralization (identified by cellular calcium production), a later stage of bone cell differentiation, was stimulated by the presence of the vertical CNWs on the surfaces. These results show that the graphene coatings, grown using the presented method, are biocompatible. And their topographies have an impact on cell behavior, which can be useful in tissue engineering applications.Electronic supplementary materialThe online version of this article (10.1007/s40820-018-0198-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…An extensive study was conducted by Bygd et al in which they screened a library of chemical functional groups based on their influence on macrophage polarization between M1 and M2 phenotypes by using poly( N ‐isopropylacrylamide‐ co ‐acrylic acid) nanoparticles modified with a variety of functional groups including phosphonic acid, alkene, epoxide, amide, ether, and nitro or oxime groups (Figure d) . In another study, a carbon nanowall surface was plasma polymerized with carbon, nitrogen, and oxygen functional groups, and the presence of oxygen functional groups increased the macrophage adhesion and activation . Similarly, polyethylene terephthalate films with hydrophilic/neutral surfaces inhibited macrophage adhesion and fusion; however, adherent macrophages on these substrates produced significantly higher amounts of various cytokines and chemokines than their hydrophobic and hydrophilic/ionic counterparts .…”

Section: Immune Responses and Immunomodulation By Biomaterialsmentioning

confidence: 99%

Current Advances in Immunomodulatory Biomaterials for Bone Regeneration

Lee

Byun

Perikamana

et al. 2018

Adv Healthcare Materials

321

267

View full text Add to dashboard Cite

Biomaterials with suitable surface modification strategies are contributing significantly to the rapid development of the field of bone tissue engineering. Despite these encouraging results, utilization of biomaterials is poorly translated to human clinical trials potentially due to lack of knowledge about the interaction between biomaterials and the body defense mechanism, the “immune system”. The highly complex immune system involves the coordinated action of many immune cells that can produce various inflammatory and anti‐inflammatory cytokines. Besides, bone fracture healing initiates with acute inflammation and may later transform to a regenerative or degenerative phase mainly due to the cross‐talk between immune cells and other cells in the bone regeneration process. Among various immune cells, macrophages possess a significant role in the immune defense, where their polarization state plays a key role in the wound healing process. Growing evidence shows that the macrophage polarization state is highly sensitive to the biomaterial's physiochemical properties, and advances in biomaterial research now allow well controlled surface properties. This review provides an overview of biomaterial‐mediated modulation of the immune response for regulating key bone regeneration events, such as osteogenesis, osteoclastogenesis, and inflammation, and it discusses how these strategies can be utilized for future bone tissue engineering applications.

show abstract

“…Briefly, the cells were seeded onto substrates at a density of 10,000 cells/cm 2 . At 2 and 24 h after seeding, the cells were processed for SEM, as previously described [19].…”

Section: Cell Morphologymentioning

confidence: 99%

Nanochannelar Topography Positively Modulates Osteoblast Differentiation and Inhibits Osteoclastogenesis

et al. 2018

Self Cite

View full text Add to dashboard Cite

Based on previously reported findings showing reduced foreign body reactions on nanochannelar topography formed on TiZr alloy, this study explores the in vitro effects of such a nanostructured surface on cells relevant for implant osseointegration, namely osteoblasts and osteoclasts. We show that such nanochannelar surfaces sustain adhesion and proliferation of mouse pre-osteoblast MC3T3-E1 cells and enhance their osteogenic differentiation. Moreover, this specific nanotopography inhibits nuclear factor kappa-B ligand (RANKL)-mediated osteoclastogenesis. The nanochannels’ dual mode of action on the bone-derived cells could contribute to an enhanced bone formation around the bone implants. Therefore, these results warrant further investigation for nanochannels’ use as surface coatings of medical implant materials.

show abstract

Surface plasma functionalization influences macrophage behavior on carbon nanowalls

Cited by 32 publications

References 40 publications

Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation

Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation

Current Advances in Immunomodulatory Biomaterials for Bone Regeneration

Nanochannelar Topography Positively Modulates Osteoblast Differentiation and Inhibits Osteoclastogenesis

Contact Info

Product

Resources

About