Surface photo-grafting of polyurethane with 2-hydroxyethyl acrylate for promotion of human endothelial cell adhesion and growth

Abstract

Cytocompatible polyurethane (PU) surface was prepared by photo-grafting 2-hydroxyethyl acrylate (HEA) onto the membrane surface. Graft polymerization was conducted by combining the use of the photo-oxidation and irradiation grafting. PU membrane was photo-oxidized to introduce the hydroperoxide groups onto the surface, then the membrane, immersed previously in monomer solution, was irradiated under UV light. The ATR-FTIR spectra, element spectroscopy for chemical analysis (ESCA), scanning electron microscopy (SEM) and water contact angle characterized the grafted copolymers and verified the occurrence of graft polymerization. The results showed that UV irradiation could realize the graft polymerization effectively and the grafting was confined within the surface layer. The grafted membrane showed minimal surface morphology. Human umbilical vein endothelial (HUVE) cells were seeded on the grafted surface. The performance of the surface in cell attachment and growth correlated with the oxygen content and mainly the carbonyl content on the surface. Cells were spread more extensively and grew faster on the surface with a higher oxygen content.

Surface engineering of electrospun polyethylene terephthalate (PET) nanofibers towards development of a new material for blood vessel engineering

“…Different methods like plasma treatment [22,23], UV irradiation [24,25] and UV-induced grafting polymerization [26,27] have been employed to surface modify polymers towards improving biocompatibility for EC. However, for surface modification of polymer nanofibers, strong reaction conditions like irradiation and plasma should be avoided because the ultrafine polymeric nanofibers are not as strong as bulk materials and can be easily destroyed.…”

Surface modification of poly‐L‐lactide by photografting of hydrophilic polymers towards improving its hydrophilicity

“…It is expected that the increase of the surface hydrophilicity and the alteration of the surface chemical composition may improve the cell compatibility of the material, as found for polyurethane membranes. 17 The functional groups, like hydroxyl and carboxyl, introduced on the PLLA surface may be used to provide a connective bridge to immobilize bioactive species, such as oligopeptides, proteins, and growth factors, and therefore, to construct a more bioactive interface. …”

“…15,16 The attachment and growth rate of human umbilical vein endothelial cells on the modified PU membranes were greatly improved. 17 Here we report the surface modification of PLLA using the same method. Hydrophilic polymers [i.e., polyhydroxyethyl methacrylate (PHEMA), poly-acrylamide (PAAm), and polymethacrylic acid (PMAA), containing OOH, OCONH 2 , and OCOOH, respectively] were immobilized onto PLLA by UV-initiated grafting copolymerization.…”

Surface modification of biomaterials by photochemical immobilization and photograft polymerization to improve hemocompatibility

“…This method has been widely used to functionalize biomaterials by the immobilization of biomacromolecules. Also, the unsaturated monomers have often been photografted on biomaterial surfaces as functional spacers to immobilize collagen, galactose ligands, succinic anhydride, and urease for indirect improvement of hemocompatibility or promotion of human endothelial cell adhesion and growth to realize endothelializated surface of biomaterials [20][21][22].…”