Surface patterning techniques for proteins on nano- and micro-systems: a modulated aspect in hierarchical structures

Bhatt, Maitri; Shende, Pravin

doi:10.1039/d1tb02455h

Cited by 11 publications

(7 citation statements)

References 158 publications

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“…The development of strategies for the organization of nanoscale components and biomolecules onto surfaces attracts significant research attention. − The spatially dictated functionalization of surfaces has demonstrated broad applicability in a range of applications including in the development of electronic devices − and sensors, , and the control of biological processes such as cell adhesion and migration. − In particular, the patterning of surfaces with DNA offers a range of opportunities based on the versatile structural and functional properties of nucleic acids, such as the ability to design molecular or cellular recognition features into DNA strands (aptamers), or engineering catalytic functions, self-assembly properties, or gene expression machineries into the DNA structures. Moreover, the conjugation of DNA strands to auxiliary functional or reporter units such as enzymes or fluorescent nanoparticles allows the DNA-guided positioning of these elements on the functionalized patterned surface.…”

Section: Applications Of Photocleavable Dna Nanostructuresmentioning

confidence: 99%

Photocleavable Ortho-Nitrobenzyl-Protected DNA Architectures and Their Applications

et al. 2023

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This review article introduces mechanistic aspects and applications of photochemically deprotected ortho-nitrobenzyl (ONB)-functionalized nucleic acids and their impact on diverse research fields including DNA nanotechnology and materials chemistry, biological chemistry, and systems chemistry. Specific topics addressed include the synthesis of the ONB-modified nucleic acids, the mechanisms involved in the photochemical deprotection of the ONB units, and the photophysical and chemical means to tune the irradiation wavelength required for the photodeprotection process. Principles to activate ONB-caged nanostructures, ONB-protected DNAzymes and aptamer frameworks are introduced. Specifically, the use of ONB-protected nucleic acids for the phototriggered spatiotemporal amplified sensing and imaging of intracellular mRNAs at the single-cell level are addressed, and control over transcription machineries, protein translation and spatiotemporal silencing of gene expression by ONB-deprotected nucleic acids are demonstrated. In addition, photodeprotection of ONB-modified nucleic acids finds important applications in controlling material properties and functions. These are introduced by the phototriggered fusion of ONB nucleic acid functionalized liposomes as models for cell–cell fusion, the light-stimulated fusion of ONB nucleic acid functionalized drug-loaded liposomes with cells for therapeutic applications, and the photolithographic patterning of ONB nucleic acid-modified interfaces. Particularly, the photolithographic control of the stiffness of membrane-like interfaces for the guided patterned growth of cells is realized. Moreover, ONB-functionalized microcapsules act as light-responsive carriers for the controlled release of drugs, and ONB-modified DNA origami frameworks act as mechanical devices or stimuli-responsive containments for the operation of DNA machineries such as the CRISPR-Cas9 system. The future challenges and potential applications of photoprotected DNA structures are discussed.

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Section: Applications Of Photocleavable Dna Nanostructuresmentioning

confidence: 99%

Photocleavable Ortho-Nitrobenzyl-Protected DNA Architectures and Their Applications

et al. 2023

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“…Progress in nanotechnology and its applications mainly rely on lithography to create finely structured surfaces that are used to improve system and device performance. As the industry experiences the optical wavelength limit of conventional photolithographic tools (based on optical sources) when reducing the feature size, nonconventional lithographic approaches that avoid time-consuming and meticulous procedures have been extensively studied to meet the increasing demand for smaller features cost-effectively. − As a result, various alternative approaches have been developed to accomplish nanoscale feature fabrication via facile, precise, and efficient patterning processes. − …”

Section: Introductionmentioning

confidence: 99%

Rapid Electrohydrodynamic-Driven Pattern Replication over a Large Area via Ultrahigh Voltage Pulses

Park,

Hwang,

Lee

et al. 2023

ACS Nano

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Despite the prospects of electrohydrodynamic instability patterning (EHIP), poor process parameter controllability is a significant challenge in uniform large-scale nanopatterning. Herein, we introduce a EHIP process using an ultrahigh electric field (>10 8 V/m) to effectively accelerate the pattern growth evolution. Owing to the strong dependence on a temporal parameter (1/τ m ) of the field strength, our method not only reduces the completion time of pattern growth but also overcomes critical parametric restrictions on the pattern replication, thereby enhancing the replicated pattern quality in three dimensions. The pattern can be uniformly replicated over the entire film surface even without a perfectly uniform air gap, which has been severely difficult in the conventional method. To further demonstrate how straightforward yet versatile our approach is, we applied our EHIP approach to successfully replicate the densely packed nanostructures of cicada wings.

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“…Microcontact printing techniques (μCPs) using PDMS stamps have been applied in many research fields, such as biopatterning, − (opto)electronics, ,− sensors, − materials science, , microfluidics, and others. Low cost and feasibility of the technique have been the reasons for the vast applications of different variations of μCP for decades.…”

Section: Introductionmentioning

confidence: 99%

Plasma Treatment of PDMS for Microcontact Printing (μCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells

Zemła,

Szydlak,

Gajos

et al. 2023

ACS Appl. Mater. Interfaces

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The present study investigates silicone transfer occurring during microcontact printing (μCP) of lectins with polydimethylsiloxane (PDMS) stamps and its impact on the adhesion of cells. Static adhesion assays and single-cell force spectroscopy (SCFS) are used to compare adhesion of nonmalignant (HCV29) and cancer (HT1376) bladder cells, respectively, to high-affinity lectin layers (PHA-L and WGA, respectively) prepared by physical adsorption and μCP. The chemical composition of the μCP lectin patterns was monitored by time-of-flight secondary ion mass spectrometry (ToF-SIMS). We show that the amount of transferred silicone in the μCP process depends on the preprocessing of the PDMS stamps. It is revealed that silicone contamination within the patterned lectin layers inhibits the adhesion of bladder cells, and the work of adhesion is lower for μCP lectins than for drop-cast lectins. The binding capacity of microcontact printed lectins was larger when the PDMS stamps were treated with UV ozone plasma as compared to sonication in ethanol and deionized water. ToF-SIMS data show that ozone-based treatment of PDMS stamps used for μCP of lectin reduces the silicone contamination in the imprinting protocol regardless of stamp geometry (flat vs microstructured). The role of other possible contributors, such as the lectin conformation and organization of lectin layers, is also discussed.

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Surface patterning techniques for proteins on nano- and micro-systems: a modulated aspect in hierarchical structures

Abstract: The surface patterning of protein using fabrication or external functionalization of structures demonstrate advanced applications in fields of biomedical research for optics, bioengineering, biosensing and antifouling. Alteration of surface structures...

Cited by 11 publications

References 158 publications

Photocleavable Ortho-Nitrobenzyl-Protected DNA Architectures and Their Applications

Photocleavable Ortho-Nitrobenzyl-Protected DNA Architectures and Their Applications

Rapid Electrohydrodynamic-Driven Pattern Replication over a Large Area via Ultrahigh Voltage Pulses

Plasma Treatment of PDMS for Microcontact Printing (μCP) of Lectins Decreases Silicone Transfer and Increases the Adhesion of Bladder Cancer Cells

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