Surface patterning of bonded microfluidic channels

Priest, Craig

doi:10.1063/1.3493643

Cited by 38 publications

(30 citation statements)

References 81 publications

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“…40 A number of methods for covalent modification in closed channel systems with reactive channel surfaces have been reported. 41 However, covalent coating on inert polymers like polystyrene or polyethylene usually requires several harsh chemical activation steps to make the surface reactive towards the coating. These pre-steps can be difficult and time consuming to make, especially in a closed microfluidic system.…”

Section: Discussionmentioning

confidence: 99%

Protein and cell patterning in closed polymer channels by photoimmobilizing proteins on photografted poly(ethylene glycol) diacrylate

2014

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Definable surface chemistry is essential for many applications of microfluidic polymer systems. However, small cross-section channels with a high surface to volume ratio enhance passive adsorption of molecules that depletes active molecules in solution and contaminates the channel surface. Here, we present a one-step photochemical process to coat the inner surfaces of closed microfluidic channels with a nanometer thick layer of poly(ethylene glycol) (PEG), well known to strongly reduce non-specific adsorption, using only commercially available reagents in an aqueous environment. The coating consists of PEG diacrylate (PEGDA) covalently grafted to polymer surfaces via UV light activation of the water soluble photoinitiator benzoyl benzylamine, a benzophenone derivative. The PEGDA coating was shown to efficiently limit the adsorption of antibodies and other proteins to <5% of the adsorbed amount on uncoated polymer surfaces. The coating could also efficiently suppress the adhesion of mammalian cells as demonstrated using the HT-29 cancer cell line. In a subsequent equivalent process step, protein in aqueous solution could be anchored onto the PEGDA coating in spatially defined patterns with a resolution of <15 μm using an inverted microscope as a projection lithography system. Surface patterns of the cell binding protein fibronectin were photochemically defined inside a closed microfluidic device that was initially homogeneously coated by PEGDA. The resulting fibronectin patterns were shown to greatly improve cell adhesion compared to unexposed areas. This method opens for easy surface modification of closed microfluidic systems through combining a low protein binding PEG-based coating with spatially defined protein patterns of interest.

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Section: Discussionmentioning

confidence: 99%

Protein and cell patterning in closed polymer channels by photoimmobilizing proteins on photografted poly(ethylene glycol) diacrylate

2014

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“…Wetting properties of the channel walls can be changed by modifying the surfaces with distinct chemical compositions. Surface chemical modifications methods, such as self‐assembly monolayer (SAM) method, plasma technology, etc., are usually combined with surface patterning technologies to modify the selected areas of the microchannel inner surfaces with different characteristics for realization of specific microscale flow control in microchannels (Figure B) …”

Section: Inner Surface Design Of Microchannelsmentioning

confidence: 99%

Inner Surface Design of Functional Microchannels for Microscale Flow Control

Wang

Yang

et al. 2019

Small

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Fluidic flow behaviors in microfluidics are dominated by the interfaces created between the fluids and the inner surface walls of microchannels. Microchannel inner surface designs, including the surface chemical modification, and the construction of micro‐/nanostructures, are good examples of manipulating those interfaces between liquids and surfaces through tuning the chemical and physical properties of the inner walls of the microchannel. Therefore, the microchannel inner surface design plays critical roles in regulating microflows to enhance the capabilities of microfluidic systems for various applications. Most recently, the rapid progresses in micro‐/nanofabrication technologies and fundamental materials have also made it possible to integrate increasingly complex chemical and physical surface modification strategies with the preparation of microchannels in microfluidics. Besides, a wave of researches focusing on the ideas of using liquids as dynamic surface materials is identified, and the unique characteristics endowed with liquid–liquid interfaces have revealed that the interesting phenomena can extend the scope of interfacial interactions determining microflow behaviors. This review extensively discusses the microchannel inner surface designs for microflow control, especially evaluates them from the perspectives of the interfaces resulting from the inner surface designs. In addition, prospective opportunities for the development of surface designs of microchannels, and their applications are provided with the potential to attract scientific interest in areas related to the rapid development and applications of various microchannel systems.

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“…Our particular interest is in using microplasma technology for spatially controlled surface treatment and thin film polymer deposition. Spatially controlled surface modification is important for the development of emerging technologies such as microfluidics, lab‐on‐a‐chip devices, biosensors and other diagnostics tools 14–16…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Operation of a Microcavity Plasma Array Device for Microscale Surface Modification

Al‐Bataineh

Szili

Gruner

et al. 2012

Plasma Processes & Polymers

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Surface modification of materials with microscale features through plasma treatment or deposition is of high value, and is considered one of the great challenges in plasma‐based materials processing. This article reports a versatile method for the fabrication of microcavity plasma array devices. A 7 × 7 microcavity plasma array device (each cavity was 250 µm in diameter and separated by 500 µm) was used in this study to demonstrate the capability of these devices for localised, non‐contact surface treatment/polymer deposition. The device can be reused multiple times for plasma treatment and polymerisation. X‐ray photoelectron spectroscopy (XPS) and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) imaging and region of interest (ROI) analysis, in addition to surface hydration, were employed to characterise the micropatterns on microplasma‐treated PS. The results showed that microplasma treatment/deposition could be spatially confined to regions exposed to the individual ignited microcavities. However, the results also demonstrated that the size of the treated spots tended to increase with increasing treatment time until they eventually overlapped resulting in a homogeneous surface treatment confined to the size of the array. Similarly, the concentration of oxygen quantified on the treated spots reached saturation after 75 s of treatment. The versatility of the device was demonstrated by depositing an array of octadiene plasma polymer (ODpp) onto a silicon substrate as confirmed by XPS imaging and ROI analysis. A key advantage of these microcavity array devices is that they can be easily integrated into manufacturing and do not require contact with the substrate surface to impart well‐defined chemical modifications on materials surfaces.

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Surface patterning of bonded microfluidic channels

Cited by 38 publications

References 81 publications

Protein and cell patterning in closed polymer channels by photoimmobilizing proteins on photografted poly(ethylene glycol) diacrylate

Protein and cell patterning in closed polymer channels by photoimmobilizing proteins on photografted poly(ethylene glycol) diacrylate

Inner Surface Design of Functional Microchannels for Microscale Flow Control

Fabrication and Operation of a Microcavity Plasma Array Device for Microscale Surface Modification

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