Surface Patterning by Microcontact Chemistry

Wendeln, Christian; Ravoo, Bart Jan

doi:10.1021/la204721x

Cited by 85 publications

(86 citation statements)

References 71 publications

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“…The shape of the chip is described in detail elsewhere. 26 For the assembly, a special roller was used. It was fabricated by an in-house workshop.…”

Section: Microspotting Of Lactose Moieties and Flow-cell Assemblingmentioning

confidence: 99%

“…The CL signal is detected by a CCD-camera, as described elsewhere. 26 In this way, 2D pictures are recorded. The CL signal intensity corresponds to the mean value of 10 pixels with the brightest signal per spot, and is referred in arbitrary units (a.u.).…”

Section: Instrumentationmentioning

confidence: 99%

“…23,24 Recently, it has become more popular for the production of microarrays. 25,26 The Munich Chip Reader 3 (MCR 3) is a stand-alone CL readout system for analytical flow-through arrays. 27 It has been used to detect 13 antibiotics in milk 28 and 4 antibiotics in honey 29 on multiplex microarrays already.…”

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confidence: 99%

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A Glyco-chip for the Detection of Ricin by an Automated Chemiluminescence Read-out System

et al. 2013

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The plant toxin ricin is a lectin that binds to D-galactose or lactose moieties by multivalent interactions. In the present work, this avidity was used to develop a novel sandwich glyco-immunoassay using a carbohydrate microarray. For realization, 6-azidohexyl-lactose was immobilized on an alkyne silane surface by Cu(I) catalyzed click chemistry. This procedure is fast, and prevents any nonspecific binding on the microarray surface. Ricin binds via its B-chain to the lactose moieties, and is detected by the biotinylated anti-ricin A-chain. By adding a horseradish peroxidase-labeled streptavidin, a chemiluminescence signal can be generated. This method is described as a sandwich-type glyco-immunoassay. The signal on the glyco-chip can be regenerated for at least 10 measurements. The limit of detection was estimated to be 80 ng mL -1 . The assay was carried out on the automated microarray readout platform MCR 3. In this way, it took 20 min for one measurement, including regeneration of the chip surface.

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“…The shape of the chip is described in detail elsewhere. 26 For the assembly, a special roller was used. It was fabricated by an in-house workshop.…”

Section: Microspotting Of Lactose Moieties and Flow-cell Assemblingmentioning

confidence: 99%

Section: Instrumentationmentioning

confidence: 99%

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A Glyco-chip for the Detection of Ricin by an Automated Chemiluminescence Read-out System

et al. 2013

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“…Ethylene glycol diglycidyl ether (EGDE) is a bifunctional epoxide compounds which has high reactivity with amines, alcohols, phenols, carboxylic acids and thiols [16]. Thus, EGDE can be used to chain extend poly(methacrylic acid), polyester, poly(imidazole) and so on [17].…”

Section: Introductionmentioning

confidence: 99%

Structure and blood compatibility of highly oriented poly(l-lactic acid) chain extended by ethylene glycol diglycidyl ether

Zhao

et al. 2015

Polymer

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Highly-oriented poly(L-lactic acid) (PLLA) were fabricated through chain extension with ethylene glycol diglycidyl ether (EGDE) and solid hot drawing technology.The extending degree as high as 25.79 mol% can be obtained at 0.7wt% EGDE content. The complex viscosity, storage and viscous modulus for chain extended PLLA were improved resulting from the enhancement of molecular entanglement, and consequently higher draw ratio can be achieved during hot stretching. The tensile strength and modulus of PLLA were improved dramatically by stretching. The stress-induced crystallization of PLLA occurred during drawing. The s.blood γ decreased by chain extension and molecular orientation, indicating the weakened interaction between bioactive substance in the blood and the surface of PLLA. Modification and orientation could significantly enhance the blood compatibility of PLLA by prolonging clotting time and decreasing hemolysis ratio, protein adsorption and platelet activation. The bionic character of oriented PLLA and its anti-coagulation mechanism were tried to be explored.

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“…Our group has shown that also simple carbohydrate microarrays can be conveniently prepared by μCP if reactive glycosides are printed on a suitable target self-assembled monolayer (SAM) [31–32]. Amongst others, we reported carbohydrate microarrays fabricated by cycloaddition of alkynes on azide-terminated SAMs [33], by Diels–Alder reaction of cyclopentadienes and furans on maleimide-terminated SAMs [34], by thiol–ene click reaction of functionalized thiols on alkene-terminated SAMs [35] as well as by strain promoted cycloadditions on azide- and nitriloxide-terminated SAMs [36] using μCP.…”

Section: Introductionmentioning

confidence: 99%

Molecular recognition of surface-immobilized carbohydrates by a synthetic lectin

Rauschenberg¹,

Fritz²,

Schulz³

et al. 2014

Beilstein J. Org. Chem.

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SummaryThe molecular recognition of carbohydrates and proteins mediates a wide range of physiological processes and the development of synthetic carbohydrate receptors (“synthetic lectins”) constitutes a key advance in biomedical technology. In this article we report a synthetic lectin that selectively binds to carbohydrates immobilized in a molecular monolayer. Inspired by our previous work, we prepared a fluorescently labeled synthetic lectin consisting of a cyclic dimer of the tripeptide Cys-His-Cys, which forms spontaneously by air oxidation of the monomer. Amine-tethered derivatives of N-acetylneuraminic acid (NANA), β-D-galactose, β-D-glucose and α-D-mannose were microcontact printed on epoxide-terminated self-assembled monolayers. Successive prints resulted in simple microarrays of two carbohydrates. The selectivity of the synthetic lectin was investigated by incubation on the immobilized carbohydrates. Selective binding of the synthetic lectin to immobilized NANA and β-D-galactose was observed by fluorescence microscopy. The selectivity and affinity of the synthetic lectin was screened in competition experiments. In addition, the carbohydrate binding of the synthetic lectin was compared with the carbohydrate binding of the lectins concanavalin A and peanut agglutinin. It was found that the printed carbohydrates retain their characteristic selectivity towards the synthetic and natural lectins and that the recognition of synthetic and natural lectins is strictly orthogonal.

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Surface Patterning by Microcontact Chemistry

Cited by 85 publications

References 71 publications

A Glyco-chip for the Detection of Ricin by an Automated Chemiluminescence Read-out System

A Glyco-chip for the Detection of Ricin by an Automated Chemiluminescence Read-out System

Structure and blood compatibility of highly oriented poly(l-lactic acid) chain extended by ethylene glycol diglycidyl ether

Molecular recognition of surface-immobilized carbohydrates by a synthetic lectin

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