Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine

Tesařová, Barbora; Dostálová, Simona; Šmídová, Veronika; Goliasova, Zita; Škubalová, Zuzana; Michalkova, Hana; Hynek, David; Michálek, Petr; Polanská, Hana; Vaculovičová, Markéta; Háček, Jaromír; Eckschlager, Tomáš; Stiborová, Marie; Pires, Ana Salomé; Neves, Rita; Abrantes, Ana M.; Rodrigues, Tiago; Matafome, Paulo; Botelho, Maria Filomena; Teixeira, Paulo; Mendes, Fernando; Heger, Zbyněk

doi:10.1016/j.apmt.2019.100501

Cited by 17 publications

(21 citation statements)

References 45 publications

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“…Besides, PEG-conjugated lipid nanoparticle (LNP) adsorbed apolipoproteins in blood and successfully delivered small interfering RNA (siRNA) to LDLR-expressed HepG2 tumors and, with the accumulation ratio of tumor to all organs up by about 30% ( Chen et al, 2019 ). Tesarova et al (2020) found that PAS [proline (P), alanine (A), and serine (S)] modified ferritin (FRT)-based nanocarriers (PAS-10-FRTElli) reduced PC formation and did not activate complement C3 in mice. They were used to deliver cytostatic alkaloid ellipticine (Elli) in vivo .…”

Section: Modulation and Applications Of In Vivo Pcmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Understanding the basic interactions between engineered nanoparticles (ENPs) and biological systems is essential for evaluating ENPs’ safety and developing better nanomedicine. Profound interactions between ENPs and biomolecules such as proteins are inevitable to occur when ENPs are administered or exposed to biological systems, for example, through intravenous injection, oral, or respiration. As a key component of these interactions, protein corona (PC) is immediately formed surrounding the outlayer of ENPs. PC formation is crucial because it gives ENPs a new biological identity by altering not only the physiochemical properties, but also the biobehaviors of ENPs. In the past two decades, most investigations about PC formation were carried out with in vitro systems which could not represent the true events occurring within in vivo systems. Most recently, studies of in vivo PC formation were reported, and it was found that the protein compositions and structures were very different from those formed in vitro. Herein, we provide an in-time review of the recent investigations of this in vivo PC formation of ENPs. In this review, commonly used characterization methods and compositions of in vivo PC are summarized firstly. Next, we highlight the impacts of the in vivo PC formation on absorption, blood circulation, biodistribution, metabolism, and toxicity of administered ENPs. We also introduce the applications of modulating in vivo PC formation in nanomedicine. We further discuss the challenges and future perspectives.

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Section: Modulation and Applications Of In Vivo Pcmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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“…For the purpose of TKIs-loading, commercially purified E. caballus spleen L-subunit-rich FRTs were utilized, with excelling biocompatibility, biodegradability, non-toxicity and high loading efficiency as has been previously demonstrated for a variety of bioactive compounds. 15,17,20,21,[32][33][34] N-PAGE revealed that the FRTs were single protein complex with an apparent MW of about 480 kDa (Figure 2A). Upon pH-dependent reassembly w/o payload, FRTs successfully retained their structural integrity and charge, which is in line with Stuhn et al 35 Similar findings were further obtained for both types of TKIs loading.…”

Section: Physico-chemical Characterization Of Tkis-loaded Frtsmentioning

confidence: 99%

“…14 In nature, the interior of FRTs is filled with iron, but when they are expressed artificially under iron-free conditions, the yielded apoFRTs are hollow, comprising a cavity that can be loaded with distinct types of bioactive compounds. [15][16][17][18][19][20][21] The uniqueness of FRTs lies in the fact that the protein can be reversibly dissociated into 24 (H-and/or L-) subunits through changes of pH from approx. 4 to 7.4 to enable encapsulation of the chosen cargo into its cavity.…”

Section: Introductionmentioning

confidence: 99%

Passive Diffusion vs Active pH-Dependent Encapsulation of Tyrosine Kinase Inhibitors Vandetanib and Lenvatinib into Folate-Targeted Ferritin Delivery System

Škubalová¹,

Rex²,

Sukupova³

et al. 2021

IJN

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“…Further, cytopathological May-Grünwald-Giemsa staining was performed upon treatment with ZnO F-H (55 µg/mL) for 24 h according to. 30 …”

Section: Methodsmentioning

confidence: 99%

The Anti-Proliferative Activity of Coordination Compound-Based ZnO Nanoparticles as a Promising Agent Against Triple Negative Breast Cancer Cells

Stepankova¹,

Świątkowski²,

Kruszyński³

et al. 2021

IJN

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Purpose The present study deals with the in vitro evaluation of the potential use of coordination compound-based zinc oxide (ZnO) nanoparticles (NPs) for the treatment of triple negative breast cancer cells (TNBrCa). As BrCa is one of the most prevalent cancer types and TNBrCa treatment is difficult due to poor prognosis and a high metastasis rate, finding a more reliable treatment option should be of the utmost interest. Methods Prepared by reacting zinc carboxylates (formate, acetate, propionate, butyrate, isobutyrate, valerate) and hexamethylenetetramine, 4 distinct coordination compounds were further subjected to two modes of conversion into ZnO NPs – ultrasonication with oleic acid or heating of pure precursors in an air atmosphere. After detailed characterization, the resulting ZnO NPs were subjected to in vitro testing of cytotoxicity toward TNBrCa and normal breast epithelial cells. Further, their biocompatibility was evaluated. Results The resulting ZnO NPs provide distinct morphological features, size, biocompatibility, and selective cytotoxicity toward TNBrCa cells. They internalize into two types of TNBrCa cells and imbalance their redox homeostasis, influencing their metabolism, morphology, and ultimately leading to their death via apoptosis or necrosis. Conclusion The crucial properties of ZnO NPs seem to be their morphology, size, and zinc content. The ZnO NPs with the most preferential values of all three properties show great promise for a future potential use in the therapy of TNBrCa.

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Surface-PASylation of ferritin to form stealth nanovehicles enhances in vivo therapeutic performance of encapsulated ellipticine

Cited by 17 publications

References 45 publications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Passive Diffusion vs Active pH-Dependent Encapsulation of Tyrosine Kinase Inhibitors Vandetanib and Lenvatinib into Folate-Targeted Ferritin Delivery System

The Anti-Proliferative Activity of Coordination Compound-Based ZnO Nanoparticles as a Promising Agent Against Triple Negative Breast Cancer Cells

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