Surface‐Modified Upconverting Microparticles and Nanoparticles for Use in Click Chemistries

Mader, Heike Sabine; Link, Martin; Achatz, Daniela E.; Uhlmann, K.; Li, Xiaohua; Wolfbeis, Otto S.

doi:10.1002/chem.201000117

Cited by 66 publications

(47 citation statements)

References 71 publications

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“…This method is based on the hydrolysis of a Si alcoxide (often tetraethylorthosilicate or modifications) in ethanolic solutions, normally in the presence of ammonia [157]. For this, the NPs have to be stabilized in ethanol media with agents such as PVP of PEG-based ligands [158,159]. This is by far the most commonly used strategy for the functionalization of PLNPs with silica that is used in bioassays [118,119,123,160].…”

Section: Functionalization Strategiesmentioning

confidence: 99%

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

et al. 2017

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Abstract:Rare earth based nanostructures constitute a type of functional materials widely used and studied in the recent literature. The purpose of this review is to provide a general and comprehensive overview of the current state of the art, with special focus on the commonly employed synthesis methods and functionalization strategies of rare earth based nanoparticles and on their different bioimaging and biosensing applications. The luminescent (including downconversion, upconversion and permanent luminescence) and magnetic properties of rare earth based nanoparticles, as well as their ability to absorb X-rays, will also be explained and connected with their luminescent, magnetic resonance and X-ray computed tomography bioimaging applications, respectively. This review is not only restricted to nanoparticles, and recent advances reported for in other nanostructures containing rare earths, such as metal organic frameworks and lanthanide complexes conjugated with biological structures, will also be commented on.

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Section: Functionalization Strategiesmentioning

confidence: 99%

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

et al. 2017

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“…Despite the validity of these methods, the functional groups are omnipresent in biological systems and cannot be labeled specifically in complex biological systems. In this sense, the so-called "click-chemistry" is an attractive alternative because the functional groups involved (e.g., azido and alkyne) are hardly present in biomolecules including proteins and oligomers and thus high selectivity and high yields of biomolecules conjugation can be guaranteed [103,104]. In addition, the strong coordination capability of the naked metal ions such as Ln 3+ on the surface of ligand-free NPs can be exploited for direct bioconjugation.…”

Section: Science China Materialsmentioning

confidence: 99%

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Huang

Zheng

et al. 2015

Sci. China Mater.

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Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide (Ln 3+ )-doped nanoparticles (NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln 3+ -doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed. INTRODUCTIONSensitive and specific bioassay of trace amount of target analytes is essential for a variety of biomedical applications ranging from pharmaceutical preparation to disease diagnosis and therapy [1][2][3]. Among various bioassay methods, luminescent bioassay has received particular attention because of its high sensitivity and good specificity [4,5]. Conventional luminescent bioassay techniques such as enzyme-linked immunosorbent assay (ELISA), time-resolved (TR) fluoroimmunoassay (TRFIA), Förster resonance energy transfer (FRET) and TR-FRET assays have laid the foundation for many modern clinical applications [6][7][8][9][10][11]. However, these bioassays are impeded by the availability of traditional bioprobes like organic dyes, lanthanide (Ln 3+ ) chelates and quantum dots (QDs). The use of these bioprobes has a number of limitations. Organic dyes commonly possess poor photochemical stability and suffer from serious photobleaching [12]. The applicability of QDs is compromised by photoblinking and high toxic- ity of heavy metal elements like cadmium and selenium, especially for in vivo biosensing [13,14]. Moreover, both organic dyes and QDs may induce high background noise and considerable photodamage to the biological samples under ultraviolet (UV) excitation, which deteriorates their detection sensitivity for bioassays [15,16]. Although such background noise can be suppressed by the technique of TR photoluminescence (PL) through the use of the longlived luminescence of Ln 3+ -chelates, the poor photochemical stability, long-term toxicity and high cost of Ln 3+ -chelates remain a major issue [17]. These concerns fuel a crucial demand for the development of a new generation of luminescent bioprobes to circumvent the limitations of traditional ones.Recently, with the explosion of nanoscience and nanotechnology, there is a growing dedication towards the development of diverse luminescent nanoprobes for biodetection ...

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“…Am häufigsten werden reaktive Aminosilane eingesetzt, um Amin-funktionalisierte UCNPs [142,143] zu erzeugen, die an Biomoleküle wie Biotin, [143] Folsäure, [144,145] Peptide, [146] Avidin, [147,148] Antikçrper [149,150] oder DNA [151,152] konjugiert werden kçnnen. Der Zusatz von 3-Azidopropyltriethoxysilan während der Silanisierung ermçglicht die Durchführung von Klick-Reaktionen auf den Nanopartikeln, [153] z. B. zur Konjugation von DNA.…”

Section: Photonen Aufkonvertierende Nanopartikelunclassified

Photonen aufkonvertierende Nanopartikel zur optischen Codierung und zum Multiplexing von Zellen, Biomolekülen und Mikrosphären

Gorris

Wolfbeis

2013

Angewandte Chemie

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Photonen aufkonvertierende Nanopartikel (photon upconverting nanoparticles, UCNPs) sind Lanthanoid‐dotierte Nanokristalle, die unter Nahinfrarotanregung sichtbares Licht emittieren (Anti‐Stokes‐Emission). Diese einzigartige optische Eigenschaft schließt Hintergrundfluoreszenz und Lichtstreuung durch biologisches Material aus. Ein weiteres Kennzeichen der UCNPs ist die Emission von mehreren schmalen Emissionslinien, was neue Wege für die optische Codierung bereitet. Eindeutige Emissionssignaturen können erzielt werden, indem man die Mehrfachemission der UCNPs durch die Zusammensetzung der Dotanden oder durch eine Oberflächenmodifikation mit Farbstoffen einstellt. Wenn nur die Intensität einer Emissionslinie justiert wird, während eine weitere Emissionslinie als konstantes Referenzsignal fungiert, können ratiometrische Codes entwickelt werden, die unabhängig von Schwankungen der absoluten Signalintensitäten sind. Die Kombination mehrerer UCNPs, die jeweils durch ihre Kennung aus Emissionslinien eindeutig identifizierbar sind, erhöht den Umfang der Codierungen exponentiell und schafft so die Voraussetzungen zur Steigerung der Mehrfachdetektion von Analyten. Dieser Aufsatz zeigt das Potenzial der UCNPs zur Markierung und Codierung von Biomolekülen, Mikrosphären und sogar ganzen Zellen auf.

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Surface‐Modified Upconverting Microparticles and Nanoparticles for Use in Click Chemistries

Cited by 66 publications

References 71 publications

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

Inorganic lanthanide nanoprobes for background-free luminescent bioassays

Photonen aufkonvertierende Nanopartikel zur optischen Codierung und zum Multiplexing von Zellen, Biomolekülen und Mikrosphären

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