Surface modified materials for active capture of enzymes

Moloney, Mark G.; Wang, Dandan; Hartz, William

doi:10.1039/d2tb02550g

Cited by 6 publications

(2 citation statements)

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“…39 Carbene surface modification has also been applied on glass fiber membranes to introduce the enzyme cellulase with successful maintenance of activity. 40 Upon thermal-or photoactivation, the diazirine motif loses N 2 and generates a reactive carbene that rapidly forms covalent bonds with any nearby available substrate through C−H, N−H, and O−H insertions without forming new high-energy species, which can lead to substrate damage. 41 Although a few studies using diazirine motifs for surface functionalization exist, their occasional application to biomolecule immobilization is limited to tedious diazirine synthesis procedures (five or seven steps) and often they are lacking optimization of the substrate.…”

Section: Introductionmentioning

confidence: 99%

“…Diazirines are effective carbene precursors that have been applied as photoaffinity labeling groups, as cross-linkers for photopatterning of polymers and semiconductors, − as adhesives for low-surface-energy materials and tissue surfaces, , as well as for transforming thermoplastic polyolefins into thermosets . Carbene surface modification has also been applied on glass fiber membranes to introduce the enzyme cellulase with successful maintenance of activity . Upon thermal- or photoactivation, the diazirine motif loses N 2 and generates a reactive carbene that rapidly forms covalent bonds with any nearby available substrate through C–H, N–H, and O–H insertions without forming new high-energy species, which can lead to substrate damage .…”

Section: Introductionmentioning

confidence: 99%

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Functionalization of Polydimethylsiloxane with Diazirine-Based Linkers for Covalent Protein Immobilization

Li,

Bi,

Musolino

et al. 2023

ACS Appl. Mater. Interfaces

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Biomolecule attachment to solid supports is critical for biomedical devices, such as biosensors and implants. Polydimethylsiloxane (PDMS) is commonly used for these applications due to its advantageous properties. To enhance the biomolecule immobilization on PDMS, a novel technique is demonstrated using newly synthesized diazirine molecules for the surface modification of PDMS. This nondestructive process involves a reaction between diazirine molecules and PDMS through C−H insertion with thermal or ultraviolet activation. The success of the PDMS modification is confirmed by various surface characterization techniques. Bovine serum albumin (BSA) and immunoglobulin G (IgG) are strongly attached to the modified PDMS surfaces, and the amount of protein is quantified using iodine-125 radiolabeling. The results demonstrate that PDMS is rapidly functionalized, and the stability of the immobilized proteins is significantly improved with multiple types of diazirine molecules and activation methods. Confocal microscopy provides three-dimensional images of the distribution of immobilized IgG on the surfaces and the penetration of diazirine-based linkers through the PDMS substrate during the coating process. Overall, this study presents a promising new approach for functionalizing PDMS surfaces to enhance biomolecule immobilization, and its potential applications can extend to multimaterial modifications for various diagnostic and medical applications such as microfluidic devices and immunoassays with relevant bioactive proteins.

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Section: Introductionmentioning

confidence: 99%