Surface-Modified Electrospun Poly(ε-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction

Sharma, Shweta; Gupta, Deepika; Mohanty, Sujata; Jassal, Manjeet; Agrawal, Ashwini K.; Tandon, Radhika

doi:10.1167/iovs.13-12727

Cited by 58 publications

(33 citation statements)

References 25 publications

(23 reference statements)

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“…Further, our findings confirm that the structural properties and architecture of the randomly deposited electrospun fibers are able to create natural ECM for cell growth and have an effect on cellular attachment and proliferation . Thus, electrospun PCL membranes alone could provide favorable growth conditions for cell survival and offer optimum nutrient and gas exchange for cell growth, even when cells have completely penetrated the scaffolds as reported by others in the case of corneal epithelial cells …”

Section: Resultssupporting

confidence: 87%

Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation

et al. 2014

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In the present study, we have fabricated electrospun poly(ε-caprolactone)-based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision wound on guinea pig completely healed within 35 days. We could reach in an assumption that the enhanced cell proliferation and wound healing might be due to the surface degradation of the polymer under physiological conditions and the formation of functional groups like hydroxyl and carboxyl groups that promoted cell proliferation in a cell adhesion protein mediated mechanism. This study is a novel tissue engineering concept for the reconstruction of a damaged tissue without the in vitro cell seeding and proliferation prior to the in vivo implantation.

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Section: Resultssupporting

confidence: 87%

Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation

et al. 2014

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“…Shweta Sharma et al electrospun PCL nanofibrous scaffold subjected to helium-oxygen (He/O 2 ) plasma treatment. The results indicated that a plasma-treated stromal equivalent can transmit 37% more light than the untreated plasma stromal equivalent [44]. Haleh Bakhshandeh et al fabricated a two-part artificial cornea as a substitute for penetrating keratoplasty in patients with corneal blindness.…”

Section: Methods Of Fabrication Of Electrospun Scaffoldsmentioning

confidence: 99%

“…Synthetic polymers, especially FDA-approved polymers, such as PLGA, PCL, and PLA (poly lactic acid), have been used in corneal tissue engineering due to their superb mechanical properties. PCL has been electrospun into nanofibrous scaffolds (Figure 1C), allowing for inoculation of limbal epithelial cells [14], human corneal epithelial cells [44], rabbit keratocytes [45] and rabbit limbal stem cells [46] on the surface of the scaffold. Studies showed that the PCL scaffold has good biocompatibility and can improve cell attachment and proliferation.…”

Section: Materials Of Electrospun Scaffoldsmentioning

confidence: 99%

“…Much research work has been performed to study how to maintain the phenotype of corneal cells, such as limbus stem cells, corneal epithelial cells and keratocytes. Limbus stem cells were demonstrated to retain a normal corneal stem cell phenotype on the electrospun silk scaffolds [51], PCL scaffolds [44,82] and blended PHBV/gelatin scaffolds [42]. The potentials of electrospun scaffolds made of polyhydroxybutyrate (PHB), PHBV, and PCL were studied to serve as drug-screening platforms for corneal keratocyte tissues by Pedram Azari et al They cultured keratocytes on these scaffolds, and the result showed that cells had a high viability and proliferation and that PCL exhibited good potential for maintaining the cell phenotype with high expressions of LUM, ALDH, VIM, COL.1, and a-SMA2 [45].…”

Section: Applications Of Electrospun Scaffolds For Corneal Tissue mentioning

confidence: 99%

“…Reproduced from [51], with permission from © 2015 Tylor and Francis; ( C ) Randomly oriented PCL scaffold. Reproduced from [44], with permission from © 2014 The Association for Research in Vision and Ophthalmology; ( D ) A section of the electrospun scaffold showing a horseshoe electrospun micro-pocket. Reproduced from [27], with permission from © 2012 Elsevier; ( E ) Electrospun nanofibrous membranes with binary COL-PEO ( a and b ) and ternary COL-HA-PEO compositions ( c and d ).…”

Section: Figurementioning

confidence: 99%

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Electrospun Scaffolds for Corneal Tissue Engineering: A Review

Kong

2016

Materials

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Corneal diseases constitute the second leading cause of vision loss and affect more than 10 million people globally. As there is a severe shortage of fresh donated corneas and an unknown risk of immune rejection with traditional heterografts, it is very important and urgent to construct a corneal equivalent to replace pathologic corneal tissue. Corneal tissue engineering has emerged as a practical strategy to develop corneal tissue substitutes, and the design of a scaffold with mechanical properties and transparency similar to that of natural cornea is paramount for the regeneration of corneal tissues. Nanofibrous scaffolds produced by electrospinning have high surface area–to-volume ratios and porosity that simulate the structure of protein fibers in native extra cellular matrix (ECM). The versatilities of electrospinning of polymer components, fiber structures, and functionalization have made the fabrication of nanofibrous scaffolds with suitable mechanical strength, transparency and biological properties for corneal tissue engineering feasible. In this paper, we review the recent developments of electrospun scaffolds for engineering corneal tissues, mainly including electrospun materials (single and blended polymers), fiber structures (isotropic or anisotropic), functionalization (improved mechanical properties and transparency), applications (corneal cell survival, maintenance of phenotype and formation of corneal tissue) and future development perspectives.

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Designing Scaffolds for Corneal Regeneration

Ahearne

Fernández‐Pérez

Masterton

et al. 2020

Adv Funct Materials

122

109

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Corneal blindness is one of the most common causes of vision loss worldwide, affecting millions of people. To treat these patients, researchers have been examining different approaches to engineer corneal scaffolds suitable for transplantation. Scaffolds have been developed to replace part or all of the cornea depending on the patient requirements. Both acellular and cell-seeded scaffolds have been tested in animal models. Materials that have been under investigation for manufacturing scaffolds include collagen, silk fibroin, amniotic membrane, decellularized cornea, fibrin, chitosan, gelatin, agarose, alginate, and hyaluronic acid in addition to several synthetic polymers. Different combinations of materials, fiber crosslinking techniques, and incorporation of bioactive molecules have also been examined. Factors such as the physical properties, cytocompatibility, degradation behavior, and optical characteristics have to be considered when selecting a suitable scaffold material. Recent advancements in materials fabrication techniques such as bioprinting, electrospinning, and different collagen alignment techniques, allow scaffolds to be generated that more accurately mimic the structure of the corneal stroma. A number of scaffolds have commenced clinical trials to determine their suitability for corneal regeneration.

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Surface-Modified Electrospun Poly(ε-Caprolactone) Scaffold With Improved Optical Transparency and Bioactivity for Damaged Ocular Surface Reconstruction

Abstract: The hydrophilicity of the surface achieved by plasma treatment effectively enhanced the transparency and promoted the biocompatibility of scaffolds. These nanofibers may act as biological cues for endorsing ocular surface engineering.

Cited by 58 publications

References 25 publications

Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation

Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation

Electrospun Scaffolds for Corneal Tissue Engineering: A Review

Designing Scaffolds for Corneal Regeneration

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