Surface Modifications in Graphene by DNA Aptamers for <i>Staphylococcus Aureus</i> Detection

Sousa, Thiago Alonso Stephan Lacerda de; Santos, Viviane Cristina Fernandes dos; Almeida, Nathalie Bonatti Franco; Santos, Flávio Augusto Portela; Silva, Thais Gomes da; Araújo, Eduardo Nery Duarte de; Andrade, Antero Silva Ribeiro de; Plentz, F.

doi:10.1109/jsen.2021.3122272

Cited by 4 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The π-conjugated units of the pyrene-modified ZIKV60 aptamers transfer electrons to graphene, resulting in the left-shift of the graphene transfer curve because of the aptamer adsorption. Several studies support this electron transfer from pyrene-modified molecules to graphene as the binding mechanism in π–π interactions between such compounds [ 35 – 37 ]. The association of pyrene-modified ZIKV60 aptamers with graphene may also be mediated by charge transfer that assists the interaction between the pyrene moiety of ZIKV60 and the π orbitals of graphene [ 38 – 39 ].…”

Section: Resultsmentioning

confidence: 93%

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Almeida¹,

Sousa²,

Santos³

et al. 2022

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

Zika virus (ZIKV) is a mosquito-borne virus that is phylogenetically close to other medically important flaviviruses with high global public health significance, such as dengue (DENV) and yellow fever (YFV) viruses. Correct diagnosis of a flavivirus infection can be challenging, particularly in world regions where more than one flavivirus co-circulates and YFV vaccination is mandatory. Acid nucleic aptamers are oligonucleotides that bind to a specific target molecule with high affinity and specificity. Because of their unique characteristics, aptamers are promising tools for biosensor development. Aptamers are usually obtained through a procedure called “systematic evolution of ligands by exponential enrichment” (SELEX). In this study, we select an aptamer (termed ZIKV60) by capillary electrophoresis SELEX (CE-SELEX) to the Zika virus non-structural protein 1 (NS1) and counterselection against the NS1 proteins of DENV (serotypes 1, 2, 3, and 4) and YFV. The ZIKV60 dissociation constant (Kd) is determined by enzyme-linked oligonucleotide assay (ELONA) and the aptamer specificity is evaluated by quantitative real-time polymerase chain reaction. ZIKV60 shows a high binding affinity to the ZIKV NS1 protein with a Kd value of 2.28 ± 0.28 nM. The aptamer presents high specificity for ZIKV NS1 compared to NS1 of DENV and YFV. Furthermore, graphene field-effect transistor devices functionalized with ZIKV60 exhibit an evident identification of NS1 protein diluted in human serum. These results point to the applicability of biosensors based on the ZIKV60 aptamer for the differential diagnosis of the Zika virus.

show abstract

Section: Resultsmentioning

confidence: 93%

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Almeida¹,

Sousa²,

Santos³

et al. 2022

Beilstein J. Nanotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

Recent advances in the detection of pathogenic microorganisms and toxins based on field-effect transistor biosensors

Feng

Xiao

et al. 2023

Critical Reviews in Food Science and Nutrition

View full text Add to dashboard Cite

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Sousa,

Almeida,

Santos

et al. 2024

Nanotechnology

Self Cite

View full text Add to dashboard Cite

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), etiological agent for the coronavirus disease 2019 (COVID-19), has resulted in over 775 million global infections. Early diagnosis remains pivotal for effective epidemiological surveillance despite the availability of vaccines. Antigen-based assays are advantageous for early COVID-19 detection due to their simplicity, cost-effectiveness, and suitability for point-of-care testing (PoCT). This study introduces a graphene field-effect transistor-based biosensor designed for high sensitivity and rapid response to the SARS-CoV-2 spike protein. By functionalizing graphene with monoclonal antibodies and applying short-duration gate voltage pulses, we achieve selective detection of the viral spike protein in human serum within 100 μs and at concentrations as low as 1 fg/mL, equivalent to 8 antigen molecules per μL of blood. Furthermore, the biosensor estimates spike protein concentrations in serum from COVID-19 patients. Our platform demonstrates potential for next-generation PoCT antigen assays, promising fast and sensitive diagnostics for COVID-19 and other infectious diseases.

show abstract

Surface Modifications in Graphene by DNA Aptamers for Staphylococcus Aureus Detection

Cited by 4 publications

References 54 publications

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

DNA aptamer selection and construction of an aptasensor based on graphene FETs for Zika virus NS1 protein detection

Recent advances in the detection of pathogenic microorganisms and toxins based on field-effect transistor biosensors

Ultrafast and highly sensitive detection of SARS-CoV-2 spike protein by field-effect transistor graphene-based biosensors

Contact Info

Product

Resources

About