Surface Modification via Plasma Polymerization of Allylamine for Antibody Immobilization

Abstract

Continuous wave and pulsed plasma polymerization coatings of allylamine were investigated for antibody immobilization as a function of plasma power, monomer pressure and treatment time, and duty cycle. Conditions were optimized by evaluating the surface amine density of plasma polymer coated samples before and after aging in dry ethanol for 3 hrs. In addition, plasma polymer coatings were characterized by contact angle analyzer, a-step, and FT-IR/ATR. The continuous wave plasma polymerization provided amine density of 4.8 molecules/nm 2 at the optimum condition of 20 W, 1 min, and 60 mTorr, while the pulsed plasma polymerization coating resulted in further increased amine density to 5.2 molecules/nm 2 at the duty cycle of 60/100.

Incorporation of Primary Amines via Plasma Technology on Biomaterials

“…The effect of plasma on the retention of the precursor functional group also depends on the plasma mode. Basarir et al [70] worked on plasma polymerization of allylamine using both CW and pulsed modes. Results showed that pulsed plasma polymerization further increased amine density.…”

“…However, the control of the cross-linking degree is an important factor for the optimization of the plasma polymer film stability and mechanical and thermal properties [72]. Therefore, it is important to evaluate the cross-linking degree in addition to the plasma and polymer film chemistry in order to choose the appropriate power [70,73].…”

“…Hamerli et al [43] and Basarir et al [70] investigated monomer flow effect on amine functionality retention. At constant power and treatment time, higher monomer flow rates yielded higher amine retention.…”

Amperimetric Biosensor Based on Carbon Nanotube and Plasma Polymer

“…Biomedical applications of allylamine PP were first reported by Muratsugu et al (Muratsugu et al, 1991) in which the F(ab') 2 fragments of antibody proteins were densely immobilized on the surface of allylamine PP. Allylamine PPs were subsequently widely used in biomedical applications (Mahoney et al, 2004;Kurosawa et al, 2002;Basarir et al, 2007;Zhang et al, 2003;Zhang et al, 2005). The PP surfaces of ethylenediamine (EDA) and alkylamines have amino groups and have also been used in biomedical applications (Jung et al, 2006;Nakanishi et al, 1996;.…”

Orientation and characterization of immobilized antibodies for improved immunoassays (Review)

“…This methodology of creating polymers allows adherent and continuous coatings to be formed on a broad range of substrates, including microtiter plates. 24 Plasma polymers have been produced from a diverse range of monomers, including allylamine, 25,26 cyclopropylamine, 27 bromine, 28 polyethylene glycol (PEG), diethylene glycol dimethyl ether (diglyme), [29][30][31] and many others, 32,33 providing a broad spectrum of chemical functionalities for subsequent protein grafting steps, including the option for patterning. [34][35][36] Overall, a significant range of polymers have been used to improve biomolecule immobilization properties of substrates for use in microarray and protein assay applications.…”