Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity

Lan, Sheeny K.; Veiseh, Mandana; Zhang, Miqin

doi:10.1016/j.bios.2004.06.025

Cited by 145 publications

(80 citation statements)

References 29 publications

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“…In addition, there is interest in controlling surface properties in a spatial and/or temporal fashion [2, 5 -10]. Regional control of surface properties can be exercised by designing substrates composed of different materials and then using self-assembling molecules specific to these materials (e.g., thiols/silane for gold/silicon [9], electrical wiring of proteins by reconstitution of cofactor modified monolayers assembled onto Au-electrodes [11], or thiols/ carboxylic acids for gold/Al 2 O 3 [2]). Another approach to controlling spatiotemporal properties of gold electrode surfaces is exemplified by works of Lahann [10] and Mrksich [8] whereby self-assembled monolayer undergoes conformational or compositional changes when electrical potential is applied, thus, resulting in switching of surface properties.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Desorption of Proteins from Gold Electrode Surface

2006

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The goal of this study was to enable rational modulation of biological interfaces using electrochemical desorption of surface-bound proteins. Coupled, surface plasmon resonance (SPR) -electrochemistry instrument was used to monitor molecular assembly events on gold electrodes and to correlate these events with changes in electrochemical properties of the substrate. Model proteins, bovine serum albumin (BSA) and immunoglobulin G (IgG), were conjugated via carbodiimide (EDC) chemistry to a layer of mercaptoundecanoic acid (MUA) assembled on SPR sensor surface. Deposition of alkanethiols and proteins were monitored by ellipsometry and SPR techniques, and was further confirmed by cyclic voltammetry with potassium ferricyanide serving as a reporter molecule. The surfacebound proteins were completely removed by applying a reductive potential of À 1200 mV vs. Pt electrode in a physiological saline buffer. Importantly, the sequence of protein immobilization followed by desorption could be repeated multiple times, thus demonstrating ability to modulate interfacial properties. Controlled removal of protein molecules from electrode surfaces is envisioned to have important applications in affinity or cell-based biosensing, cellular micropatterning and cell sorting.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Desorption of Proteins from Gold Electrode Surface

2006

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“…Biofouling, in the case of biomaterials is the initial step of cell adhesion, that is, protein adsorption. The proteins get adsorbed to the surface of the materials through physical or chemical interactions [25]. It is observed that hydrophilic surfaces have better tendency to adsorb proteins molecules [26].…”

Section: Surface Modifications By Immobilization Of Moleculesmentioning

confidence: 99%

Fundamentals of Surface Modification

2014

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“…While some earlier pioneering studies in this field have focused on polymeric materials (Hanein et al, 2001;Owen et al, www.ecmjournal.org E Kaivosoja et al Micro-patterned surfaces 2005;Biggs et al, 2007a;Biggs et al, 2007b;Hart et al, 2007;Kunzler et al, 2007), with only one study conducted on metallic materials patterned on a silicon background (Berry et al, 2007). Some other cell types have also been evaluated, in particular on gold-silicon textured surfaces (Lan et al, 2005;Veiseh et al, 2004). Further to this, only neuroblastoma cells have previously been investigated on diamond like carbon (DLC)-silicon micro-textured surfaces (Kelly et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of silicon using micro-patterned surfaces of thin films

Kaivosoja

Myllymaa²,

Kouri³

et al. 2010

eCM

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Micro-textured biomaterials might enhance cytocompatibility of silicon-based micro-electromechanical system (bio-MEMS) dummies. Photolithography-physical vapour deposition was used to produce diamond-like carbon (DLC) or Ti squares and circles on silicon, and also their inverse replicas; then DLC and Ti were compared for their guiding potential, using a SaOS-2 cell model. Scanning electron microscopy at 48 hours indicated cells were well-spread on large-sized patterns (several cells on one pattern) and assumed the geometrical architecture of underlying features. Mediumsized patterns (slightly smaller than solitary indicator cells) were inhabited by singular cells, which stretched from one island to another, assuming longitudinal or branching morphologies. On small-sized patterns (much smaller than individual cells) cells covered large micro-textured areas, but cellular filopodia bypassed the bare silicon. Immunofluorescence and confocal laser scanning microscopy indicated that the actin cytoskeleton and vinculin-containing adhesion junctions were present on the patterned areas, but not on the bare silicon. Cell density/ coverage disclosed a 3.4-3.7-fold preference for the biomaterial patterns over silicon substrate (p < 0.001). Differences in the cellular response between materials were lost at 120 hours when cells were confluent. The working hypothesis was proven; enhancement by micro-patterning depends on the pattern size, shape and material and can be used to improve biocompatibility during the initial integration phase of the device.

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Surface modification of silicon and gold-patterned silicon surfaces for improved biocompatibility and cell patterning selectivity

Cited by 145 publications

References 29 publications

Electrochemical Desorption of Proteins from Gold Electrode Surface

Electrochemical Desorption of Proteins from Gold Electrode Surface

Fundamentals of Surface Modification

Enhancement of silicon using micro-patterned surfaces of thin films

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