Surface modification of polystyrene biochip for biotin‐labelled protein/streptavidin or neutravidin coupling used in fluorescence assay

Gao, Xin; Mathieu, H; Schawaller, Manfred

doi:10.1002/sia.1920

Cited by 15 publications

(5 citation statements)

References 23 publications

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“…Pure streptavidin should have a nitrogen content of 12% at 398 eV. [26] However, it has to be considered that streptavidin is expected to be only immobilized inside the pores while the surface of the honeycomb-structured porous film consisted mainly of polystyrene. The nitrogen content measured should therefore be below the nitrogen content of the protein.…”

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confidence: 99%

Microwells with Patterned Proteins by a Self‐Assembly Process Using Honeycomb‐Structured Porous Films

2008

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confidence: 99%

Microwells with Patterned Proteins by a Self‐Assembly Process Using Honeycomb‐Structured Porous Films

2008

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“…Biomarker detection represents an important task for many scientific fields such as disease diagnosis, , biodefense, environmental monitoring, and biological research . Biomarkers are molecular or cellular indicators that are used to measure and evaluate biological states of the target subjects.…”

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confidence: 99%

Label-Free Biomarker Assay in a Microresistive Pulse Sensor via Immunoaggregation

Han

Liu

et al. 2014

Anal. Chem.

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We present a label-free biomarker detection method based on immunoaggregation and resistive pulse sensing technology. In this approach, target biomarkers and antibody (Ab)-functionalized microparticles are mixed to form biomarker-microparticle aggregates. A resistive pulse sensor is then used to measure the sizes and count the number of aggregates. The measured volume fraction of the aggregates represents the concentration of the targeted biomarker. In our tests, human ferritin, used as a biomarker, triggered the aggregation of antiferritin Ab-functionalized microparticles in phosphate-buffered saline (PBS). The volume fraction of aggregates increased with the increased ferritin concentration. We also demonstrated the detection of human ferritin in 10% fetal bovine serum (FBS) to mimic a real detection environment in complex media. The detection range from 0.1 to 208 ng/mL was achieved. In addition, we demonstrated that the detection range can be shifted to lower and higher concentrations by decreasing and increasing microparticle concentrations. This biomarker detection method is label-free, rapid, and able to quantitatively measure the concentration of any macromolecular biomarker as long as an antibody can be found, with simple measurement setup and sample preparations.

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“…Rapid advancements in biosensor technology over the past several decades has led to widespread implementation across a variety of application fields, ranging from biodefense (Hindson et al, 2005;Ivnitski et al, 2003;Lim et al, 2005;Peruski and Peruski, 2003), to medical diagnostics (Andreotti et al, 2003;Gao et al, 2004), to environmental monitoring (Baeumner, 2003;Farre and Barcelo, 2003). The diversity of applications and sample matrices results in a nonuniform set of operational requirements.…”

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confidence: 99%

Three-Dimensional Near Infrared Imaging of Pathophysiological Changes Within the Breast

Yalavarthy¹

2008

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A significant challenge for all biosensor systems is to achieve high assay sensitivity and specificity while minimizing sample preparation requirements, operational complexity, and sample-to-answer time. We have achieved multiplexed, unamplified, femtomolar detection of both DNA and proteins in complex matrices (including whole blood, serum, plasma, and milk) in minutes using as few as two reagents by labeling conventional assay schemes with micrometerscale magnetic beads, and applying fluidic force discrimination (FFD). In FFD assays, analytes captured onto a microarray surface are labeled with microbeads, and a controlled laminar flow is then used to apply microfluidic forces sufficient to preferentially remove only nonspecifically bound bead labels. The density of beads that remain bound is proportional to the analyte concentration and can be determined with either optical counting or magnetoelectronic detection of the magnetic labels. Combining FFD assays with chip-based magnetoelectronic detection enables a simple, potentially handheld, platform capable of both nucleic acid hybridization assays and immunoassays, including orthogonal detection and identification of bacterial and viral pathogens, and therefore suitable for a wide range of biosensing applications. Report Documentation Page Form Approved OMB No. 0704-0188Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. A significant challenge for all biosensor systems is to achieve high assay sensitivity and specificity while minimizing sample preparation requirements, operational complexity, and sample-to-answer time. We have achieved multiplexed, unamplified, femtomolar detection of both DNA and proteins in complex matrices (including whole blood, serum, plasma, and milk) in minutes using as few as two reagents by labeling conventional assay schemes with micrometerscale magnetic beads, and applying fluidic force discrimination (FFD). In FFD assays, analytes captured onto a microarray surface are labeled with microbeads, and a controlled laminar flow is then used to apply microfluidic forces sufficient to preferentially remove only nonspecifically bound bead labels. The density of beads that remain bound is proportional to the analyte concentration and can be determined with either optical co...

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Surface modification of polystyrene biochip for biotin‐labelled protein/streptavidin or neutravidin coupling used in fluorescence assay

Cited by 15 publications

References 23 publications

Microwells with Patterned Proteins by a Self‐Assembly Process Using Honeycomb‐Structured Porous Films

Microwells with Patterned Proteins by a Self‐Assembly Process Using Honeycomb‐Structured Porous Films

Label-Free Biomarker Assay in a Microresistive Pulse Sensor via Immunoaggregation

Three-Dimensional Near Infrared Imaging of Pathophysiological Changes Within the Breast

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