Surface modification of nylon membrane by glycidyl methacrylate graft copolymerization for antibody immobilization

Abstract

Surface of nylon membrane was modified by the graft copolymerization of glycidyl methacrylate (GMA) using persulfate and thiosulfate as redox initiator system. Effect of various reaction parameters such as initiator concentration, monomer concentration, polymerization time, and temperature on degree of grafting was also studied. Maximum grafting of 100% was achieved by using equimolar concentration (0.008M) of redox initiator and 0.5M of GMA monomer at 70 C in 60 min. Grafted nylon membranes with various graft levels of GMA were characterized by various techniques such as fourier transform infrared spectroscopy, thermo gravimetric analysis, and scanning electron microscopy. The GMA grafted nylon (NyM-g-GMA) membranes with different graft levels were evaluated as a support for immobilization of rabbit anti goat antibody (RAG IgG). Antibody (Ab) immobilized NyM-g-GMA membranes were evaluated using ELISA and Bradford protein estimation method. Nylon membrane with 60% graft level showed optimum immobilization of Ab at RAG IgG conc. of 0.625 lg/mL with low nonspecific binding. Maximum immobilization efficiency (I.E.%) of 56% was observed for membrane with 60% graft level at 50 lg/ mL of RAG IgG in PBS (pH 7.4). Ab immobilized NyMg-GMA discs were found to be stable up to 6 weeks at 4 C and 2 days at 37

Biomolecule-functionalized polymer brushes

“…Thus, the epoxide group-containing polymers are well-suited for the immobilization of proteins, enzymes and other biomolecules. [18][19][20] The most common epoxy polymer brush is based on poly(glycidyl methacrylate) (PGMA). PGMA is a known surface linker and spacer for biomolecules.…”

Polyacrylonitrile Fiber as Matrix for Immunodiagnostics

“…Many polymeric materials such as polyethylene, nitrocellulose (NC), Dacron, polyvinyl chloride (PVC), nylon, polyacrylonitrile (PAN) etc. have been widely studied as bioassay's matrix over the years as a reliable interface between materials and biological moieties [Charles, et al, 2006;Jackeray, et al, 2010;Jain, et al, 2008;Venditti, et al,2008].…”

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

“…Other types of biomolecules, such as antibodies [27] or DNA [28], were also successfully immobilized on a polymer membrane with the aim of developing highly active surfaces. A rabbit anti-goat antibody was successfully covalently immobilized on a nylon-grafted-glycidyl methacrylate polymer.…”