Surface Modification of Multiple Bioactive Peptides to Improve Endothelialization of Vascular Grafts

Ge, Peng; Yao, Danyu; Niu, Yimeng; Liu, Haifeng; Fan, Yubo

doi:10.1002/mabi.201800368

Cited by 37 publications

(24 citation statements)

References 38 publications

(52 reference statements)

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“…Fibers with smaller diameters offer a higher surface area, which can support a more homogenous cell distribution and spreading (27). However, some studies have demonstrated that small variations in diameter of fibers, such as those observed in the present study, do not modify the interaction of cells with the scaffolds (9,28). Therefore, it is believed that the differences in cell morphology obtained for the two groups of scaffolds have been caused mainly by their different rates of TMC and LA.…”

Section: Discussioncontrasting

confidence: 64%

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

Braghirolli

Caberlon

Gamba

et al. 2019

Braz J Med Biol Res

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Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate- co -L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60 showed smooth fibers with an average diameter of 771±273, 606±242, and 697±232 nm, respectively. When the degradation ratio was evaluated, the three scaffold groups had a similar molecular weight ( M w ) on the final day of analysis. PTMCLLA 30/70 and 40/60 scaffolds exhibited greater flexibility than the PTMCLLA 20/80. However, the PTMCLLA 40/60 scaffolds showed a large wrinkling and their biological properties were not evaluated. The PTMCLLA 30/70 scaffolds supported the adhesion and growth of mesenchymal stem cells (MSCs), endothelial progenitor cells, and smooth muscle cells (SMCs). In addition, they provided a spreading of MSCs and SMCs. Given the results, the electrospun scaffolds produced with PTMCLLA 30/70 copolymer can be considered promising candidates for future applications in vascular tissue engineering.

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Section: Discussioncontrasting

confidence: 64%

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

Braghirolli

Caberlon

Gamba

et al. 2019

Braz J Med Biol Res

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“…The micropore structure in the wall of ASDBVs is considered to be the premise of endothelialization , which is recognized as one of the most effective methods of long‐term anti‐thrombosis of vascular substitutes . At present, the simple and rapid method to achieve endothelialization of ASDBVs has been the focus of many researchers .…”

Section: Discussionmentioning

confidence: 99%

Small diameter blood vessels with controllable micropore structure induced by centrifugal force for improved endothelialization

Gao

Chen

et al. 2020

Engineering in Life Sciences

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The micropore structure is prerequisite for fast and durable endothelialization of artificial small diameter blood vessels (ASDBVs). Although some methods, such as salt leaching, coagulation, and electrospinning, have been developed to construct micropores for ASDBVs, the uncontrollability of the structure and the complicated procedures of the process are still the issues to be concerned about. In this study, a compact device based on the principle of centrifugal force is established and used to prepare polyurethane (PU) ASDBVs with micropore structures by blasting different porogens. It is found that the glass beads could construct micropores with regular round shape, uniform distribution, and controllable size (60-350 µm), which significantly improves the endothelialization of PU-based ASDBVs, especially when the pore size is about 60 µm. This method is easy-accessible and wide-applicable, which provides a new pathway for the research and development of ASDBVs. K E Y W O R D Scentrifugal force, endothelialization, micropore structure, polyurethane, small diameter blood vessel

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“…The Arg-Gly-Asp (RGD) peptide, present in fibronectin and responsible for strong cell adhesion, is very efficient in promoting EC adhesion and their migration in vascular grafts [ 75 ]. Moreover, the combination of RGD with peptides occurring in the laminin β chain, such as YIGSR, significantly enhances EC proliferation [ 76 ]. GFPGER, a protein sequence occurring in collagen type I, increases endothelialization more efficiently than collagen and laminin and even reduces platelet adhesion in an ex vivo arteriovenous shunt model under antiplatelet therapy [ 73 ].…”

Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

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Insufficient endothelialization of cardiovascular grafts is a major hurdle in vascular surgery and regenerative medicine, bearing a risk for early graft thrombosis. Neither of the numerous strategies pursued to solve these problems were conclusive. Endothelialization is regulated by the endothelial basement membrane (EBM), a highly specialized part of the vascular extracellular matrix. Thus, a detailed understanding of the structure–function interrelations of the EBM components is fundamental for designing biomimetic materials aiming to mimic EBM functions. In this review, a detailed description of the structure and functions of the EBM are provided, including the luminal and abluminal interactions with adjacent cell types, such as vascular smooth muscle cells. Moreover, in vivo as well as in vitro strategies to build or renew EBM are summarized and critically discussed. The spectrum of methods includes vessel decellularization and implant biofunctionalization strategies as well as tissue engineering-based approaches and bioprinting. Finally, the limitations of these methods are highlighted, and future directions are suggested to help improve future design strategies for EBM-inspired materials in the cardiovascular field.

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Surface Modification of Multiple Bioactive Peptides to Improve Endothelialization of Vascular Grafts

Cited by 37 publications

References 38 publications

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts

Small diameter blood vessels with controllable micropore structure induced by centrifugal force for improved endothelialization

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

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