Surface modification of lipid-based nanocarriers for cancer cell-specific drug targeting

Kim, Chang‐Hyun; Lee, Sang Gon; Kang, Myung Joo; Lee, Sang Kil; Choi, Young Wook

doi:10.1007/s40005-017-0329-5

Cited by 109 publications

(47 citation statements)

References 189 publications

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“…The notable reduction of cellular uptake of CPC/rGO after pretreatment with CPE30 supports the idea that CPC/ rGO were taken up by claudin 4 receptor-mediated endocytosis ( Figure 6). The competition assay results indicate that the presence of CPE30 in CPC conjugates may have contributed to overcoming the cellular uptake-limiting PEG dilemma [23] , and provide claudin 4 receptor-specific delivery.…”

Section: Discussionmentioning

confidence: 98%

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

et al. 2017

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In this study we designed a claudin 4-directed dual photodynamic and photothermal system, in which a 30-amino acid claudin 4-binding peptide, Clostridium perfringens enterotoxin (CPE), was linked to a photodynamic agent chlorin e6 (Ce6) through a polyethylene glycol spacer (CPC) and anchored onto reduced graphene oxide (rGO) nanosheets to form CPC/rGO nanosheets. For comparison, a conjugate of polyethylene glycol and Ce6 (PC) was anchored onto the rGO nanosheets to generate PC/rGO. Both PC and CPC generated reactive oxygen species upon irradiation at 660 nm. Application of CPC/rGO to claudin 4-overexpressing U87 glioblastoma cells in vitro resulted in a significantly higher cellular uptake compared to application of PC/rGO. Upon irradiation at 660 and 808 nm, the CPC/rGO-treated U87 cells generated significantly higher reactive oxygen species and caused significantly higher temperature increase, and showed most potent anticancer effect compared to the other groups. Taken together, these results suggest that CPC/rGO is potentially useful as a tumor-specific combined phototherapy.

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Section: Discussionmentioning

confidence: 98%

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

et al. 2017

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“…After the tumor volume reached 150−200 mm 3 , Cy5.5-loaded NPs (dispersed in 0.1 mL of normal saline) were injected into the tail vein of the mouse. The tumor volume (V, mm 3 ) was calculated using the following formula: V =0.5× longest diameter × (shortest diameter). 2 A laser diode with a wavelength of 670 nm was used to excite the Cy5.5 dye.…”

Section: Nirf Imagingmentioning

confidence: 99%

“…Diverse organic (natural and synthetic) and inorganic materials have been introduced as the matrix of nanovehicles. [1][2][3][4][5][6][7] Among various organic polymers, poly((D,L)lactic-glycolic)acid (PLGA) is the most popular material because of its biocompatibility and biodegradability and was approved by the US Food and Drug Administration. However, PLGA does not possess a tumor targeting moiety.…”

Section: Introductionmentioning

confidence: 99%

Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting

Park¹,

Cho²,

Kim³

2017

IJN

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Poly((D,L)lactic-glycolic)acid–star glucose (PLGA-Glc) polymer-based nanoparticles (NPs) were fabricated for tumor-targeted delivery of docetaxel (DCT). NPs with an approximate mean diameter of 241 nm, narrow size distribution, negative zeta potential, and spherical shape were prepared. A sustained drug release pattern from the developed NPs was observed for 13 days. Moreover, drug release from PLGA-Glc NPs at acidic pH (endocytic compartments and tumor regions) was significantly improved compared with that observed at physiological pH (normal tissues and organs). DCT-loaded PLGA-Glc NPs (DCT/PLGA-Glc NPs) exhibited an enhanced antiproliferation efficiency rather than DCT-loaded PLGA NPs (DCT/PLGA NPs) in Hep-2 cells, which can be regarded as glucose transporters (GLUTs)-positive cells, at ≥50 ng/mL DCT concentration range. Under glucose-deprived (hypoglycemic) conditions, the cellular uptake efficiency of the PLGA-Glc NPs was higher in Hep-2 cells compared to that observed in PLGA NPs. Cy5.5-loaded NPs were prepared and injected into a Hep-2 tumor-xenografted mouse model for in vivo near-infrared fluorescence imaging. The PLGA-Glc NPs group exhibited higher fluorescence intensity in the tumor region than the PLGA NPs group. These results imply that the PLGA-Glc NPs have active tumor targeting abilities based on interactions with GLUTs and the hypoglycemic conditions in the tumor region. Therefore, the developed PLGA-Glc NPs may represent a promising tumor-targeted delivery system for anticancer drugs.

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“…The liposomal drug delivery system has been widely used to achieve intracellular delivery of various anti-cancer agents, owing to several advantages, including feasible encapsulation of both hydrophilic and lipophilic drugs and excellent biocompatibility and/or biodegradability [8]. However, its lack of selectivity to specific cancer cells continues to serve as a barrier for tumor targeting [9].…”

Section: Introductionmentioning

confidence: 99%

“…However, its lack of selectivity to specific cancer cells continues to serve as a barrier for tumor targeting [9]. To resolve this drawback, liposomes can be surface-modified with different ligands, including peptides, antibodies, small molecules, and carbohydrates [8]. As folate receptors (FRs) are strongly upregulated in different tumors [10], folic acid (FA) is an attractive ligand for targeted delivery.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Intracellular Delivery of BCG Cell Wall Skeleton into Bladder Cancer Cells Using Liposomes Functionalized with Folic Acid and Pep-1 Peptide

et al. 2019

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Although bacillus Calmette–Guérin cell wall skeleton (BCG-CWS) might function as a potential substitute for live BCG, its use in the treatment of bladder cancer remains limited owing to issues such as insolubility and micrometer-size following exposure to an aqueous environment. Thus, to develop a novel nanoparticulate system for efficient BCG-CWS delivery, liposomal encapsulation was carried out using a modified emulsification-solvent evaporation method (targets: Size, <200 nm; encapsulation efficiency, ~60%). Further, the liposomal surface was functionalized with specific ligands, folic acid (FA), and Pep-1 peptide (Pep1), as targeting and cell-penetrating moieties, respectively. Functionalized liposomes greatly increased the intracellular uptake of BCG-CWS in the bladder cancer cell lines, 5637 and MBT2. The immunoactivity was verified through elevated cytokine production and a THP-1 migration assay. In vivo antitumor efficacy revealed that the BCG-CWS-loaded liposomes effectively inhibited tumor growth in mice bearing MBT2 tumors. Dual ligand-functionalized liposome was also superior to single ligand-functionalized liposomes. Immunohistochemistry supported the enhanced antitumor effect of BCG-CWS, with IL-6 production and CD4 infiltration. Thus, we conclude that FA- and Pep1-modified liposomes encapsulating BCG-CWS might be a good candidate for bladder cancer treatment with high target selectivity.

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Surface modification of lipid-based nanocarriers for cancer cell-specific drug targeting

Cited by 109 publications

References 189 publications

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting

Enhanced Intracellular Delivery of BCG Cell Wall Skeleton into Bladder Cancer Cells Using Liposomes Functionalized with Folic Acid and Pep-1 Peptide

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Surface modification of lipid-based nanocarriers for cancer cell-specific drug targeting

Cited by 109 publications

References 189 publications

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate

Poly((D,L)lactic-glycolic)acid&ndash;star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting

Enhanced Intracellular Delivery of BCG Cell Wall Skeleton into Bladder Cancer Cells Using Liposomes Functionalized with Folic Acid and Pep-1 Peptide

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Product

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Poly((D,L)lactic-glycolic)acid–star glucose nanoparticles for glucose transporter and hypoglycemia-mediated tumor targeting