Surface modification of doxorubicin-loaded nanoparticles based on polydopamine with pH-sensitive property for tumor targeting therapy

Abstract

One major challenge of current surface modification of nanoparticles is the demand for chemical reactive polymeric layers, such modification is always complicated, inefficient, and may lead the polymer lose the ability to encapsulate drug. To overcome this limitation, we adopted a pH-sensitive platform using polydopamine (PDA) as a way of functionalizing nanoparticles (NPs) surfaces. All this method needed to be just a brief incubation in weak alkaline solution of dopamine, which was simple and applicable to a variety of polymer carriers regardless of their chemical reactivity. We successfully conjugated the doxorubicin (DOX)-PDA-poly (lactic-co-glycolic acid) (PLGA) NPs with two typical surface modifiers: folate (FA) and a peptide (Arg-Gly-Asp, RGD). The DOX-PDA-FA-NPs and DOX-PDA-RGD-NPs (targeting nanoparticles) were characterized by particle size, zeta potential, and surface morphology. They were quite stable in various physiological solutions and exhibited pH-sensitive property in drug release. Compared to DOX-NPs, the targeting nanoparticles possessed an excellent targeting ability against HeLa cells. In addition, the in vivo study demonstrated that targeting nanoparticles achieved a tumor inhibition rate over 70%, meanwhile prominently decreased the side effects of DOX and improve drug distribution in tumors. Our studies indicated that the DOX-PLGA-NPs modified with PDA and various functional ligands are promising nanocarriers for targeting tumor therapy.

Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites

“…32 Additionally, PDA can spontaneously form a conformal layer through in situ polymerization in an alkaline dopamine solution. 33 In this study, we fabricated Fe 3 O 4 @PDA composite superparticles by employing preassembled Fe 3 O 4 nanoparticles as the core and PDA as the shell, with the PDA coating further improving the physiological stability and biocompatibility of the Fe 3 O 4 superparticles. In vitro experiments indicated that the Fe 3 O 4 @PDA composite superparticles exhibited no adverse effects on MSC characteristics and increased the number of MSCs in the S-phase, their proliferation index and migration ability, and their secretion of vascular endothelial growth factor (VEGF).…”

Acidity and Glutathione Dual‐Responsive Polydopamine‐Coated Organic‐Inorganic Hybrid Hollow Mesoporous Silica Nanoparticles for Controlled Drug Delivery

“…Notably, this coating is very sensitive to pH, allowing drug molecules to be blocked in HMONs at neutral pH and released at lower pH. [12][13][14] The surface of HMONs is usually modified with a hydrophilic polymer polyethylene glycol (PEG) to maximize drug delivery to the tumor. [15][16][17] However, surface modification may be very difficult and cumbersome due to the lack of chemically reactive functional groups on the surface of HMONs.…”

Minocycline-Modified Pure Titanium Has Good Antibacterial Properties and Improves the Osteogenesis of Bone Marrow Stem Cells

“…In this study, we developed a minocycline-coated pure titanium as an antibacterial material and a guidance material for bone regeneration. We used polydopamine (PD) as the coating medium for minocycline coating [19,20] . The objectives of this study were to evaluate the antibacterial properties of minocycline-coated pure titanium and to investigate its effects on the viability and osteogenic differentiation of human mesenchymal stem cells (hMSCs).…”