Surface modification-assisted bonding of polymer-based microfluidic devices

Tennico, Yolanda H.; Koesdjojo, Myra T.; Kondo, Shu; Mandrell, David; Remcho, Vincent T.

doi:10.1016/j.snb.2009.10.001

Cited by 86 publications

(68 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since normal adhesives cannot be used for the bonding of PDMS a combination of bonding and clamping is used to build first prototypes. The adhesive problem can be addressed by modified bonding techniques (KS Lee and Ram 2009;Tang and Lee 2010;Vlachopoulou et al 2009;Sunkara et al 2011;Tennico et al 2010) or PDMS interface bonding (Suk et al 2008;Chow et al 2006). The actuator is insensitive to bonding quality because not all the paraffin needs to be molten for closure of the valve and the remaining solid paraffin will seal the cavity.…”

Section: Microvalve Assemblymentioning

confidence: 99%

A layered modular polymeric μ-valve suitable for lab-on-foil: design, fabrication, and characterization

Boustheen

Homburg

Somhorst

et al. 2011

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

Section: Microvalve Assemblymentioning

confidence: 99%

A layered modular polymeric μ-valve suitable for lab-on-foil: design, fabrication, and characterization

Boustheen

Homburg

Somhorst

et al. 2011

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…This was followed by surface modification of both PMMA substrates using oxygen plasma and tetraethyl orthosilicate (TEOS ≥ 99.0%). 49 The two substrates were brought into contact and pressed together with a hot press for 20 min at 75 °C and 300 psi to form the final microchip. Finally, the bonded chip was baked at 60 °C for 2 h to increase the bond strength.…”

Section: Methodsmentioning

confidence: 99%

10 000‐fold concentration increase in proteins in a cascade microchip using anionic ITP by a 3‐D numerical simulation with experimental results

et al. 2011

View full text Add to dashboard Cite

This paper describes the preconcentration of the biomarker cardiac troponin I (cTnI) and a fluorescent protein (R-phycoerythrin) using cationic isotachophoresis (ITP) in a 3.9 cm long poly(methyl methacrylate) (PMMA) microfluidic chip. The microfluidic chip includes a channel with a 5× reduction in depth and a 10× reduction in width. Thus, the overall cross-sectional area decreases by 50× from inlet (anode) to outlet (cathode). The concentration is inversely proportional to the cross-sectional area so that as proteins migrate through the reductions, the concentrations increase proportionally. In addition, the proteins gain additional concentration by ITP. We observe that by performing ITP in a cross-sectional area reducing microfluidic chip we can attain concentration factors greater than 10 000. The starting concentration of cTnI was 2.3 μg mL–1 and the final concentration after ITP concentration in the microfluidic chip was 25.52 ± 1.25 mg mL–1. To the author's knowledge this is the first attempt at concentrating the cardiac biomarker cTnI by ITP. This experimental approach could be coupled to an immunoassay based technique and has the potential to lower limits of detection, increase sensitivity, and quantify different isolated cTnI phosphorylation states.

show abstract

“…16 In the bonding process, first a second piece of PMMA was trimmed to size, cleaned and dried ( Fig. 2(l)).…”

Section: Microchip Fabricationmentioning

confidence: 99%

A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation

et al. 2012

View full text Add to dashboard Cite

Recent studies show that reduction in cross-sectional area can be used to improve the concentration factor in microscale bioseparations. Due to simplicity in fabrication process, a step reduction in cross-sectional area is generally implemented in microchip to increase the concentration factor. But the sudden change in crosssectional area can introduce significant band dispersion and distortion. This paper reports a new fabrication technique to form a gradual reduction in cross-sectional area in polymethylmethacrylate (PMMA) microchannel for both anionic and cationic isotachophoresis (ITP). The fabrication technique is based on hot embossing and surface modification assisted bonding method. Both one-dimensional and twodimensional gradual reduction in cross-sectional area microchannels were formed on PMMA with high fidelity using proposed techniques. ITP experiments were conducted to separate and preconcentrate fluorescent proteins in these microchips. Thousand fold and ten thousand fold increase in concentrations were obtained when 10 Â and 100 Â gradual reduction in cross-sectional area microchannels were used for ITP.

show abstract

Surface modification-assisted bonding of polymer-based microfluidic devices

Cited by 86 publications

References 28 publications

A layered modular polymeric μ-valve suitable for lab-on-foil: design, fabrication, and characterization

A layered modular polymeric μ-valve suitable for lab-on-foil: design, fabrication, and characterization

10 000‐fold concentration increase in proteins in a cascade microchip using anionic ITP by a 3‐D numerical simulation with experimental results

A new fabrication technique to form complex polymethylmethacrylate microchannel for bioseparation

Contact Info

Product

Resources

About