Surface modification and antibacterial properties of superelastic Ti-Zr-based alloys for medical application

Konopatsky, Anton S.; Teplyakova, Tatyana; Popova, Daria V.; Vlasova, Kseniya Yu.; Прокошкин, С. Д.; Shtansky, Dmitry V.

doi:10.1016/j.colsurfb.2021.112183

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…Accordingly, due to these irregularities, stretching or buckling of the membrane causes higher tension and stress, facilitating rupture of the bacterial wall. Several groups have proven an antimicrobial effect on E. coli, S. aureus, P. vulgaris and other pathogens on nanostructured surfaces which had a similar design to our respective surfaces [14,21,30,31]. In the mentioned studies the initial adhesion of Gramnegative and Gram-positive bacteria was markedly inhibited in the first 6 h by the surface described above [10,27,30].…”

Section: Discussionmentioning

confidence: 72%

“…Recently Wang et al, could show an increased soft-tissue adhesion after long-term acid etching of titanium [20]. Acid solutions, such as piranha solution, have been shown to result in a porous surface that could enhance bactericidal activity through its mechanical properties and chemical state [21]. Although several studies have shown antimicrobial properties in vitro, most tested the properties against E. coli [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…Acid solutions, such as piranha solution, have been shown to result in a porous surface that could enhance bactericidal activity through its mechanical properties and chemical state [21]. Although several studies have shown antimicrobial properties in vitro, most tested the properties against E. coli [21][22][23][24]. However, in contrast to P. gingivalis, E. Coli is not associated with peri-implant diseases.…”

Section: Introductionmentioning

confidence: 99%

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Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

et al. 2021

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Objectives: The transgingival part of titanium implants is either machined or polished. Cell-surface interactions as a result of nano-modified surfaces could help gingival fibroblast adhesion and support antibacterial properties by means of the physico-mechanical aspects of the surfaces. The aim of the present study was to determine how a nanocavity titanium surface affects the viability and adhesion of human gingival fibroblasts (HGF-1). Additionally, its properties against Porphyromonas gingivalis were tested. Material and Methods: Two different specimens were evaluated: commercially available machined titanium discs (MD) and nanostructured discs (ND). To obtain ND, machined titanium discs with a diameter of 15 mm were etched with a 1:1 mixture of 98% H2SO4 and 30% H2O2 (piranha etching) for 5 h at room temperature. Surface topography characterization was performed via scanning electron microscopy (SEM) and atomic force microscopy (AFM). Samples were exposed to HGF-1 to assess the effect on cell viability and adhesion, which were compared between the two groups by means of MTT assay, immunofluorescence and flow cytometry. After incubation with P. gingivalis, antibacterial properties of MD and ND were determined by conventional culturing, live/dead staining and SEM. Results: The present study successfully created a nanostructured surface on commercially available machined titanium discs. The etching process created cavities with a 10–20 nm edge-to-edge diameter. MD and ND show similar adhesion forces equal to about 10–30 nN. The achieved nanostructuration reduced the cell alignment along machining structures and did not negatively affect the proliferation of gingival fibroblasts when compared to MD. No differences in the expression levels of both actin and vinculin proteins, after incubation on MD or ND, were observed. However, the novel ND surface failed to show antibacterial effects against P. gingivalis. Conclusion: Antibacterial effects against P. gingivalis cannot be achieved with nanocavities within a range of 10–20 nm and based on the piranha etching procedure. The proliferation of HGF-1 and the expression levels and localization of the structural proteins actin and vinculin were not influenced by the surface nanostructuration. Further studies on the strength of the gingival cell adhesion should be performed in the future. Clinical relevance: Since osseointegration is well investigated, mucointegration is an important part of future research and developments. Little is known about how nanostructures on the machined transgingival part of an implant could possibly influence the surrounding tissue. Targeting titanium surfaces with improved antimicrobial properties requires extensive preclinical basic research to gain clinical relevance.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

et al. 2021

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“…The Ti 2p doublet was deconvoluted into two components. The main contributions are from peaks at 458.6–458.8 and 464.4–464.5 eV with a spin–orbit gap (Δ E ) of approximately 5.8 eV, which is typical for TiO 2 . The best fit for the Ti 2p spectrum is achieved by adding an additional component at a higher binding energy (BE) value (460 eV), which can be attributed to the Ti 4+ peak from the oxidized surface. , Analysis of the XPS O 1s spectrum allows us to assess the presence of nanoparticle hydration.…”

Section: Resultsmentioning

confidence: 99%

Highly Hydrophilic TiO₂ Nanoparticles as Stabilizers of Pickering Emulsions with Photosensitive Lipophilic Compounds: Synthesis and Application

Barilyuk,

Korol,

Chikanova

et al. 2024

J. Phys. Chem. B

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Pickering emulsions are a very promising system for encapsulating and photoprotecting active ingredients. The highest photoprotection efficiency can be achieved when bare TiO 2 nanoparticles are used as stabilizers. However, the main problem when using highly hydrophilic TiO 2 nanoparticles is their inability to adsorb at the oil−water interface. Here, we developed emulsions stabilized by bare, highly hydrophilic TiO 2 nanoparticles for the encapsulation and photoprotection of active lipophilic compounds. Emulsion stabilization occurs due to the formation of hydrogen bonds between hydroxyl groups on the particle surface and the carbonyl groups of the oil molecules. The stability and rheological properties of emulsions are explained by the properties of the initial hydrosols. The resulting Pickering emulsions demonstrated effective UV protection of α-lipoic acid. Our results pave the way for the formulation of Pickering emulsions with a widely used cosmetic oil and show for the first time the possibility of photoprotection of a lipophilic active substance using unmodified TiO 2 nanoparticles.

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“…It indicates that the ALD coating was successfully formed on the surface of Ti-18Zr-15Nb alloy. The natural oxide layer contains all main components of the alloy [62], in our case Zr and Nb. The silver content in the Ag/ALD sample is quite low and noticeably higher for Ag(d)/ALD, which is in a good agreement with the EDX results.…”

Section: Surface Chemical Statementioning

confidence: 99%

Surface Modification of Biomedical Ti-18Zr-15Nb Alloy by Atomic Layer Deposition and Ag Nanoparticles Decoration

Konopatsky

Teplyakova

Sheremetyev

et al. 2023

JFB

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Superelastic biocompatible alloys attract significant attention as novel materials for bone tissue replacement. These alloys are often composed of three or more components that lead to the formation of complex oxide films on their surfaces. For practical use, it is desirable to have a single-component oxide film with a controlled thickness on the surface of biocompatible material. Herein we investigate the applicability of the atomic layer deposition (ALD) technique for surface modification of Ti-18Zr-15Nb alloy with TiO2 oxide. It was found that a 10–15 nm thick, low-crystalline TiO2 oxide layer is formed by ALD method over the natural oxide film (~5 nm) of the Ti-18Zr-15Nb alloy. This surface consists of TiO2 exclusively without any additions of Zr or Nb oxides/suboxides. Further, the obtained coating is modified by Ag nanoparticles (NPs) with a surface concentration up to 1.6% in order to increase the material’s antibacterial activity. The resulting surface exhibits enhanced antibacterial activity with an inhibition rate of more than 75% against E.coli bacteria.

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Surface modification and antibacterial properties of superelastic Ti-Zr-based alloys for medical application

Cited by 10 publications

References 35 publications

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

Highly Hydrophilic TiO₂ Nanoparticles as Stabilizers of Pickering Emulsions with Photosensitive Lipophilic Compounds: Synthesis and Application

Surface Modification of Biomedical Ti-18Zr-15Nb Alloy by Atomic Layer Deposition and Ag Nanoparticles Decoration

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Surface modification and antibacterial properties of superelastic Ti-Zr-based alloys for medical application

Cited by 10 publications

References 35 publications

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

Effect of a Nanostructured Titanium Surface on Gingival Cell Adhesion, Viability and Properties against P. gingivalis

Highly Hydrophilic TiO2 Nanoparticles as Stabilizers of Pickering Emulsions with Photosensitive Lipophilic Compounds: Synthesis and Application

Surface Modification of Biomedical Ti-18Zr-15Nb Alloy by Atomic Layer Deposition and Ag Nanoparticles Decoration

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Highly Hydrophilic TiO₂ Nanoparticles as Stabilizers of Pickering Emulsions with Photosensitive Lipophilic Compounds: Synthesis and Application