Surface membrane remodeling following removal of vasopressin in toad urinary bladder

Mia, A.J.; Oakford, L.X.; Yorio, Thomas

doi:10.1016/0040-8166(94)90094-9

Cited by 7 publications

(16 citation statements)

References 26 publications

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“…Following stimulation of bladder sacs with ADH, both control and experimental bladder sacs received two quick serosal buffer rinses at the appropriate temperature for withdrawal of hormone action, and the sacs were then allowed to recover or retrieve for 15 min, 30 min, or 60 min to allow for endocytosis and apical membrane remodeling to occur (22). These experiments were performed at 25°C and 15°C prior to tissue fixation.…”

Section: Methodsmentioning

confidence: 99%

“…An osmotic gradient was established between the serosal and mucosal sides using a 1/10 dilution of Ringer's solution of the mucosal cavity. Vasopressin at a concentration of 100 mU/ml was then added to the serosal bathing solution of the experimental bladder sacs for 15 min as in previous studies (21,22). ADH stimulation for 10 or 15 rnin at room temperature induces maximum water flow as well as membrane fusion events with a limited membrane downregulation in toad urinary bladders (9, 10, 16, 19,22,30), whereas prolonged ADH stimulation causes spontaneous apical membrane remodeling and return to a state similar to reactivation (25).…”

Section: Methodsmentioning

confidence: 99%

“…For analysis of the percentage of cells showing surface invaginations, tissues were selected randomly, and scanning electron micrographs were taken at 1500x and 3000x magnifications from areas with clear cellular delineations as described previously (22). As before, the total number of normal and invaginated cells for each treatment were counted separately and averaged to show the results in percentages (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Tissue blocks were polymerized overnight in an oven at 60°C. Ultrathin sections made with a diamond knife were collected on bare copper or nickel grids and exposed to uranyl acetate and lead citrate for staining and TEM studies (22). All TEM pictures were made at 31,50Ox, unless otherwise noted.…”

Section: Methodsmentioning

confidence: 99%

“…Tissue processing for SEM and TEM was carried out as described previously (10, 22). For comparative analysis, all SEM pictures were taken at 1500x and at 3000x magnifications with appropriate increases in the printing images.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Temperature on Apical Membrane Remodeling in ADH-Stimulated Toad Urinary Bladders

Mia

Oakford

Wood

et al. 1998

Experimental Biology and Medicine

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Pretreatment and removal of vasopressin (ADH) in toad urinary bladder renal model tissues induces endocytosis at 25 degrees C. The objective of the current study is to determine if apical membrane remodeling, as well as transepithelial water flow, can be affected by lowering the temperature to 15 degrees C. Control toad urinary bladders in the presence of an osmotic gradient at either 25 degrees C or 15 degrees C when visualized by scanning electron microscopy (SEM) show a typical apical membrane surface with no apparent surface differences. ADH-treated tissues following 15-min stimulation at 25 degrees C or 15 degrees C revealed a propagation of apical microvilli on their surface membranes. After 15 min following removal of ADH, bladder tissues at 25 degrees C or 15 degrees C showed surface invaginations involving over 44% and 80% of granular cells, respectively. The rate of water flow in tissues at 15 degrees C remained elevated compared to tissues held at 25 degrees C. This was consistent with the observation that ADH-stimulated tissues following washout at 15 degrees C still had marked apical membrane surface involvement. However, at 30 min and 60 min postwashout, ADH-stimulated tissues at 15 degrees C recovered considerably, with a reduction in the number of shallow apical membrane invaginations involving fewer than 33% and 20% of granular cells respectively. This may indicate that the membrane undergoes continuous remodeling even at cold temperature conditions but with a different half-time. Control bladder tissues subjected to transmission electron microscopy (TEM) reveal a dense cytoplasmic profile with a scattered distribution of secretory granules, rough ER cisternae, mitochondria, and little or no vacuolation. In contrast, ADH-stimulated bladder tissues displayed a vacuolated cytoplasm, expanded rough ER cisternae, and ruffled basolateral membranes. These observations suggest that the apical membrane undergoes considerable reorganization following cessation of hormone action and that lowering the temperature reduces the rate of membrane remodeling and thus may provide a means to monitor the processes of endocytosis and the mechanisms responsible for water channel retrieval.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Temperature on Apical Membrane Remodeling in ADH-Stimulated Toad Urinary Bladders

Mia

Oakford

Wood

et al. 1998

Experimental Biology and Medicine

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Vasopressin-induced morphological changes in polarized rat hepatocyte multiplets: Dual calcium-dependent effects

et al. 2008

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Presence of Caveolae in the Granular Epithelial Cells of Rabbit Urinary Bladder

1997

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Previously, we reported the presence of caveolae, clathrin-coated pits and vesicles in toad urinary bladder granular epithelial cells, and in smooth muscle and endothelial cells. Since then we investigated the occurrence of caveolae in the rabbit urinary bladder cells. Here we report for the first time the presence of caveolae and clathrin-coated pits and vesicles in the granular epithelial, smooth muscle and endothelial cells of rabbit urinary bladders.Urinary bladder sacs were surgically removed from doubly pithed toads and anaesthetized rabbits, and allowed fixation for 1 hr in 2% glutaraldehyde in PIPES buffer. These bladder tissues were divided for scanning (SEM) and transmission electron microscopy (TEM) for comparative analysis. A postfixation using 1% osmium tetroxide was carried out for 1 hr. Tissues were appropriately processed for SEM and TEM observations. Ultrathin sections, made with a diamond knife, were taken on bare nickel grids and then exposed to uranyl acetate and lead citrate for viewing in the TEM.

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Surface membrane remodeling following removal of vasopressin in toad urinary bladder

Cited by 7 publications

References 26 publications

Effect of Temperature on Apical Membrane Remodeling in ADH-Stimulated Toad Urinary Bladders

Effect of Temperature on Apical Membrane Remodeling in ADH-Stimulated Toad Urinary Bladders

Vasopressin-induced morphological changes in polarized rat hepatocyte multiplets: Dual calcium-dependent effects

Presence of Caveolae in the Granular Epithelial Cells of Rabbit Urinary Bladder

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