Surface megalin expression is a target to the inhibitory effect of bradykinin on the renal albumin endocytosis

Acute Kidney Injury Caused by Rhabdomyolysis Is Ameliorated by Serum Albumin-Based Supersulfide Donors through Antioxidative Pathways

“…Megalin and cubilin mediate the uptake of serum albumin via endocytosis in renal proximal tubule cells [ 47 , 48 ]. Since megalin and cubilin are expressed in LLC-PK1 cells [ 49 , 50 ], it is hypothesized that S n -HSA transferred supersulfides via megalin or cubilin in this study. However, megalin and cubilin might not recognize poly-NAC-Sn-HSA as serum albumin because more than five lysine residues of HSA are modified.…”

SARS-CoV-2 spike protein inhibits megalin-mediated albumin endocytosis in proximal tubule epithelial cells

“…Megalin has been shown to be a target of different compounds involved in the modulation of albumin reabsorption in PTECs [23] , [40] . Furthermore, some syndromes linked to changes in the function of PTECs such as Donnai-Barrow syndrome and Dent disease, have decreased surface megalin expression associated with an albuminuria phenotype [42] , [43] .…”

“…Total megalin expression was measured by immunofluorescence as described previously [23] . LLC-PK1 and HEK-293A cells were grown in coverslips until 60 %–70 % confluence was reached.…”

“…The assessment of cellular megalin trafficking was performed as published previously [23] . Initially, LLC-PK1 cells, stably transfected with megT0-HA, were seeded and grown in coverslips until reach 60 %–70 % confluence was reached.…”

“…Flow cytometry was performed as published previously [23] . After incubation of LLC-PK1 and HEK-293A cells with S protein, the cells were washed out twice with PBS, harvested with trypsin/EDTA for 3 min and centrifuge at 2500 × g for 5 min.…”