Surface markers of human natural killer cells as analyzed in a modified single cell cytotoxicity assay on poly-l-lysine coated cover slips

Vargas‐Cortes, Mauricio; Hellström, U.; Perlmann, Peter

doi:10.1016/0022-1759(83)90114-x

Cited by 44 publications

(27 citation statements)

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“…Individuals with persistently low NK activity were described as prone to the development of malignancy 10,12,13 . The level of NK activity is considered as a stable individual trait, with fluctuates within a low, middle or high normal range characteristic for each individual, remaining the same unless disease intervenes [13][14][15][16][17] .…”

Section: Discussionmentioning

confidence: 99%

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Familial cancer: depressed NK-cell cytotoxicity in healthy and cancer affected members

Montelli

Peraçoli

Gabarra

et al. 2001

Arq. Neuro-Psiquiatr.

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Depressed natural killer (NK) cell activity has been showed in family members of patients with different types of cancer. The present work aimed to evaluate T cell subsets and NK cell cytotoxic activity in 15 members of a family with high incidence of tumors, such as glioblastoma, gastric, pancreas and colon rectal carcinoma, chronic myelocitic leukemia, melanoma and osteoblastoma. As controls, 19 healthy subjects with the age range equivalent were studied. The enumeration of CD3+ lymphocytes and their CD4+ and CD8+ subsets were defined by monoclonal antibodies and NK cell cytotoxicity towards K562 target cells were evaluated by single cell-assay. The results showed in family members low percentage of total T cells (CD3+), and their CD4+ subset and impairment of CD4/CD8 ratio in relation to control group. All family members presented percentage of NK-target cell conjugate formation bellow the minimum value observed in control group. Thirteen people were examined and followed up during five years, in order to assure that there was no undiagnosed or unsuspected disease at the moment of evaluation. One of them developed osteoblastoma and other malignant melanoma. Two cancer patients, with glioblastoma and chronic myelocytic leukemia were studied during illness. All the corresponding values were comparable. The persistence of low percentage of conjugate formation may be related to a defect on adhesion molecules expression in the surface of NK cells that was probably responsible for the low activity of these cells presented by the family group. Thus, the inheritance mechanism of low adherence of NK cells should have a prognostic value in determining the risk of developing tumors.

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Section: Discussionmentioning

confidence: 99%

“…The single-cell cytotoxicity assay on PLL-coated coverslips was performed as described by Vargas-Cortes et al 10 with minor modifications. Effector cell/target cell conjugates were formed by mixing 100ml of the effector cell suspension with an equal …”

Section: Target Cellsmentioning

confidence: 99%

Familial cancer: depressed NK-cell cytotoxicity in healthy and cancer affected members

Montelli

Peraçoli

Gabarra

et al. 2001

Arq. Neuro-Psiquiatr.

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“…PBL cells and unlabelled K562 cells were used as effector and target cells, respectively. The single-cell cytotoxicity assay on PLL-coated coverslips was performed as described by Vargas-Cortes et al 20 with minor modifications. Effector cell/target cell conjugates were formed by mixing 100ml of the effector cell suspension with an equal volume of target cells, both at a concentration of 1x10 6 cells/ml.…”

Section: Target Cellsmentioning

confidence: 99%

Immunological alterations in patients with primary tumors in central nervous system

Peraçoli

Montelli

Soares

et al. 1999

Arq. Neuro-Psiquiatr.

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-Natural killer (NK) cells play an important role in immune surveillance against tumors. The present work aimed to study the cytotoxic activity of NK cells and T cell subsets in peripheral blood of 13 patients with primary tumors in central nervous system (CNS). As controls 29 healthy subjects with the age range equivalent to the patients were studied. The methods employed were: a) determination of cytotoxic activity of NK cells towards K562 target cells, evaluated by single cell-assay; b) enumeration of CD3+ lymphocytes and their CD4+ and CD8+ subsets defined by monoclonal antibodies; c) the identification of tumors were done by histologic and immunochemistry studies. The results indicated that adults and children with tumor in CNS display reduced percentage of total T cells, helper/inducer subset and low helper/suppressor ratio. The cytotoxic activity of NK cells was decreased in patients with CNS tumors due mainly to a decrease in the proportion of targetbinding lymphocytes. These results suggest that cytotoxic activity of NK cells may be affected by the immunoregulatory disturbances observed in patients with primary tumors in CNS. KEY WORDS: brain tumors, NK cells, T cell subsets.Alterações imunológicas em pacientes com tumores primários no sistema nervoso central RESUMO -As células natural killer (NK) desempenham importante papel na vigilância imunológica contra tumores. O objetivo do presente trabalho foi estudar a atividade citotóxica de células NK e as subpopulações de células T no sangue periférico de 13 pacientes com tumores primários no sistema nervoso central (SNC). Como controle foram estudados 29 indivíduos saudáveis com faixa etária equivalente aos pacientes. Os métodos empregados foram: a) determinação da atividade citotóxica de células NK contra células alvo K562; b) quantificação de linfócitos CD3+ e subpopulações CD4+ e CD8+ por meio de anticorpos monoclonais; c) identificação dos tumores por análise histológica e imuno-histoquímica. Os resultados indicaram que adultos e crianças com tumores no SNC apresentam diminuição na percentagem de linfócitos T, da subpopulação de células T auxiliares e da relação células auxiliares/supressoras. A atividade citotóxica de células NK esteve deprimida em pacientes com tumores devido principalmente à diminuição da capacidade de formar conjugados com a célula alvo K562. Os resultados sugerem que a atividade de células NK pode ser afetada por distúrbios imunorregulatórios observados em pacientes com tumores primários no SNC. PALAVRAS-CHAVE: tumores cerebrais, células NK, subpopulações de células T.Most cancers result from interaction of genetic and environment factors; however, genetic factors by themselves explains only about 5% of all cancer. The others have been atributed to environmental factors, that may interact with genetic cancer susceptibility and individual response 1 .

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“…Single cell binding assay The method of Vargas-Cortez et al [19] cases) and CD3, CD4 expression was very low ( < 1%) ( Table 2). Less than 20% of cells in these cell lines were co-expressing CD8 marker.…”

Section: Nkcf Assaysmentioning

confidence: 99%

Natural killer (NK) cell activity during HIV infection: a decrease in NK activity is observed at the clonal level and is not restored afterin vitrolong-term culture of NK cells

Scott‐Algara

Vuillier

Cayota

et al. 1992

Clinical and Experimental Immunology

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SUMMARYNK cell activity is impaired in HIV-infected patients. The mechanisms behind the altered NK functions are not clear, and conflicting data concerning NK and antibody-dependent cellular cytotoxicity (ADCC) activity have been reported. In order to investigate whether this impairment is also observed at the clonal level and whether it is related to a defect at the target cell binding and/or the post-binding level, we evaluated highly purified NK cell lines and cloned NK cells obtained from 22 HIV-infected patients at different stages of disease and compared them with normal controls for their ability to: (i) kill K-562 and U-937 cell lines using a 51Cr release assay; (ii) bind and kill K-562 and U-937 cells at the single cell binding level; (iii) release NK cytotoxic factor (NKCF), tumour necrosis factor-alpha (TNF-ax) and interferon-gamma (IFN-y); (iv) kill anti-IgM preincubated Daudi cell line (ADCC activity). This study with cloned NK cells or NK cell lines from HIV-infected individuals showed: (i) a decrease in their lytic capability against target cell lines; (ii) a low ability to form conjugates with K-562 and U-937 cell lines with respect to controls; (iii) a decreased ability to kill bound target cells; (iv) low levels of released NKCF, TNF-a and IFN-y after incubation with U-937 cells. Taken together, these findings suggest that the impaired NK cell function during HIV infection is also observed at the clonal level and is related to defects both at the target and post-binding levels. However, the precise mechanisms remain to be determined. The inability to restore normal NK activity after long-term culture in the presence of high levels of recombinant IL-2 is in agreement with the hypothesis of a 'general anergic process' during HIV infection.

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Surface markers of human natural killer cells as analyzed in a modified single cell cytotoxicity assay on poly-l-lysine coated cover slips

Cited by 44 publications

References 43 publications

Familial cancer: depressed NK-cell cytotoxicity in healthy and cancer affected members

Familial cancer: depressed NK-cell cytotoxicity in healthy and cancer affected members

Immunological alterations in patients with primary tumors in central nervous system

Natural killer (NK) cell activity during HIV infection: a decrease in NK activity is observed at the clonal level and is not restored afterin vitrolong-term culture of NK cells

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