Surface markers of human gingival fibroblasts in vitro. Characterization and modulation by enzymes and bacterial products

Abstract

Surface markers of human gingival fibroblasts in vitro were investigated using monoclonal and helerologous unlisera against a range of cell surface antigens, together with resetting techniques to characterize surface receptors for IgG and C., WI‐38 fibroblasts and human peripheral blood monocytes were used as control cells. Human gingival fibroblasts exhibited complement receptors and (β2‐micro‐globulin, as did W1–3S cells. Ten per cent of the human gingival fibroblasts were positive for HLA‐DR antigens and additionally exhibited a granulocyte antigen not apparent on WI‐3K cells. Monolayers of the gingival fibroblasts were further exposed for short periods to varying concentrations of enzymes (trypsin, collagenase and neuraminidase), bacterial extracts (lipopolysaccharide and lipoteichoic acid) and crude supra‐ and subgingival plaque sonicates. Surface‐marker analysis was then carried out. The most noticeable effects were obtained with Vibrio cholerae neuraminidase which enhanced C3, receptor and surface antigen expression, and supragingival plaque sonicate which depressed the expression of HLA‐DR and granulocytc antigens while not affecting β2microglobulin expression. Trypsin reduced antigen expression to a degree, but its effects were mainly on cell adherence.

Growth, viability, adhesion potential, and fibronectin expression in fibroblasts cultured on zirconia or feldspatic ceramics in vitro

“…In normal cells, adhesion contributes to the regulation of cellular movement, division and survival. 21,22 In particular, since it has been shown that cellular adhesion to substrate is directly related to the growth rate of cultured cells, 23,[43][44][45] the analysis of this parameter is considered essential in biocompatibility studies in vitro. 46,47 We used a sensitive CyQuant 1 assay 23 to measure cell adhesion, and observed that the number of adherent cells was significantly higher (þ43%, p < 0.01) on zirconia that on FE ceramic already after 16-h incubation.…”

Lymphocyte-Fibroblast Interactions

“…Although it has not been directly demonstrated that HLA-DR antigens were expressed on HGF in periodontal lesions, Barber et al (1984) found that HGF, which were derived from explants of periodontally diseased tissue, expressed HLA-DR antigens in vitro. Moreover, we have demonstrated that high levels of IFNy mRNA were detectable in inflamed gingival tissue (Shimabukuro et al, 1996).…”

The effect of laser‐treated titanium surface on human gingival fibroblast behavior

“…31,32 As for primary HGFs, it should be noted that there is little data on markers of these cells in the Ref. 33. Therefore, to identify their behavior and generate meaningful conclusions, the established primary HGF cell line was characterized.…”