Surface lectin binding characteristics of developing stages of Brugia in Armigeres subalbatus: I. Brugia pahangi.

Abstract

Abstract:Surface characteristics of microfilariae and developing stages of Brugia pahangi in its natural vector, Armigeres subalbatus were assayed using fluorescein isothiocynate conjugated lectins. The following lectins were used: wheat germ agglutinins, lentil agglutinins, Helix aspersa agglutinins, Concanavalin A agglutinins, kidney bean agglutinins, asparagus pea agglutinins and pea nut agglutinins. It was observed that developing stages of B. pahangi the mosquito showed a dynamic surface carbohydrate characteristics.The larvae change their surface coat configuration frequently during the 10 day observation period. However, blood dwelling microfilariae and fully matured infective larvae obtained from the mosquito's head showed little or no binding affinity for the lectins tested. It's postulated that the rapid turnover of the surface carbohydrates, while development of the larvae is taking place, is the worm's response to a 'hostile' mosquito environment.