Surface hydrolysis of fibrous poly(ε-caprolactone) scaffolds for enhanced osteoblast adhesion and proliferation

Park, Jeong‐Soo; Kim, Jung Man; Lee, Sung Jun; Lee, Se Geun; Jeong, Young Keun; Kim, Sung Eun; Lee, Sang Cheon

doi:10.1007/bf03218809

Cited by 68 publications

(47 citation statements)

References 26 publications

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“…After the treatment the contact angle of the scaffold became 0. However, contrary to literature reports for other polymers, such as polycaprolactone, the hydrolysis process induced some deformation of the fibers, and the fibers reduced their diameter. The scanning electron microscopy analysis [Fig.…”

Section: Discussioncontrasting

confidence: 98%

Influence of random and oriented electrospun fibrous poly(lactic‐co‐glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

Sperling

Reis

Pozzobon

et al. 2017

J Biomedical Materials Res

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Engineering neural tissue by combining biodegradable materials, cells and growth factors is a promising strategy for the treatment of central and peripheral nervous system injuries. In this study, neural differentiation of mouse embryonic stem cells (mESCs) was investigated in combination with three dimensional (3D) electrospun nanofibers as a substitute for the extracellular matrix (ECM). Nano/microfibrous poly(lactic-co-glycolic acid) (PLGA) 3D scaffolds were fabricated through electrospinning and characterized. The scaffolds consisted of either a randomly oriented or an aligned structure of PLGA fibers. The mESCs were induced to differentiate into neuronal lineage and the effect of the polymer and fiber orientation on cell survival, morphology and differentiation efficiency was studied. The neural progenitors derived from the mESCs could survive and migrate onto the fibrous scaffolds. Aligned fibers provided more contact guidance with the neurites preferentially extending along the long axis of fiber. The mESCs differentiated into neural lineages expressing neural markers as seen by the immunocytochemistry. The nestin and beta3-tubulin expression was enhanced on the PLGA aligned fibers in comparison with the other groups, as seen by the quantitative analysis. Taken together, a combination of electrospun fiber scaffolds and mESC derived neural progenitor cells could provide valuable information about the effects of topology on neural differentiation and axonal regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1333-1345, 2017.

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Section: Discussioncontrasting

confidence: 98%

Influence of random and oriented electrospun fibrous poly(lactic‐co‐glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

Sperling

Reis

Pozzobon

et al. 2017

J Biomedical Materials Res

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“…18,19 Arg-Gly-Asp (RGD) peptide was found to be the major functional amino acid sequence, presenting in all major ECM proteins and it has been known to be associated with the attachment and spreading of ECs. [20][21][22][23] As an another EC-adhesive molecule, laminin derived Tyr-Ile-Gly-Ser-Arg (YIGSR) peptide was often used for promoting endothelialization.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

et al. 2009

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Polyurethane (PU) is widely used as a cardiovascular biomaterial due to its good mechanical properties and hemocompatibility, but it is not adhesive to endothelial cells (ECs). Cell adhesive peptides, GRGDS and YIGSR, were found to promote adhesion and spreading of ECs and showed a synergistic effect when both of them were used. In this study, a surface modification was designed to fabricate an EC-active PU surface capable of promoting endothelialization using the peptides and poly(ethylene glycol) (PEG) spacer, The modified PU surfaces were characterized in vitro. The density of the grafted PEG on the PU surface was measured by acid-base back titration to the terminal-free isocyanate groups. The successful immobilization of peptides was confirmed by amino acid analysis, following hydrolysis, and contact angle measurement. The uniform distribution of peptides on the surface was observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). To evaluate the EC adhesive property, cell viability test using human umbilical vein EC (HUVEC) was investigated in vitro and enhanced endothelialization was characterized by the introduction of cell adhesive peptides, GRGDS and YIGSR, and PEG spacer. Therefore, GRGDS and YIGSR co-immobilized PU surfaces can be applied to an EC-specific vascular graft with long-term patency by endothelialization.

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“…The alkaline hydrolyzed scaffolds were still rather hydrophobic. The hydrolysis conducted at such low temperatures as 45 o C (for post‐HPCL scaffold) and 50 o C (for pre‐HPCL scaffold) did not seem to efficiently induce the scission of polymer chains, yielding carboxylate (–COOH) and hydroxyl (–OH) groups on the HPCL chain termini . The plasma treatment, however, revealed a more prominent effect on the enhancement of surface hydrophilicity than the alkaline hydrolysis.…”

Section: Resultsmentioning

confidence: 95%

“…Recently, a great deal of attention has been paid to the surface modification of polymeric scaffolds in order to improve the surface properties of the materials and, hence, to enhance cell–material interactions. Such methods as alkaline hydrolysis and plasma treatment of polyester scaffolds were proved to increase the surface hydrophilicity of the resulting materials and significantly enhance cell adhesion, proliferation, and function.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of chondrocyte proliferation, distribution, and functions within polycaprolactone scaffolds by surface treatments

Uppanan

Thavornyutikarn

Kosorn

et al. 2014

J. Biomed. Mater. Res.

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Enhancement of porcine chondrocyte growth, distribution and functions within polycaprolactone (PCL) scaffolds was attempted using alkaline hydrolysis and oxygen plasma treatment. The hydrolysis of PCL was performed either before or after scaffold fabrication in the preparations of pre-hydrolyzed PCL (pre-HPCL) or post-HPCL scaffolds, respectively. The PCL, pre-HPCL, and post-HPCL scaffolds were subsequently plasma-treated to yield plasma-treated PCL, plasma-treated pre-HPCL, and plasma-treated post-HPCL scaffolds, respectively. All scaffolds were comparatively characterized, in terms of surface morphology, hydrophilicity, and atomic composition using scanning electron microscopy, contact angle measurement and X-ray photoelectron spectroscopy, respectively. The interactions of chondrocytes with individual scaffolds were assessed, in terms of cartilage-gene expression and cartilaginous matrix production using reverse transcription polymerase chain reaction analysis and glycosaminoglycans (GAGs) assay, respectively. The cell infiltration and cartilaginous matrix distribution were investigated by histological and immunofluorescence analysis. The results revealed that the plasma treatment exhibited a more prominent effect on the enhancement of surface roughness and hydrophilicity of the scaffolds than the alkaline hydrolysis. The scaffolds subjected to both surface treatments stimulated the cells to secret more GAGs and type II collagen. The sequence of hydrolysis of PCL also evidently played a crucial role in the hydrophilicity of the materials and the cartilage-gene expression and cartilaginous matrix production of the cultured chondrocytes. The hydrolysis of PCL prior to the fabrication, followed by the oxygen plasma treatment of the resulting fabricated scaffold, yielded plasma-treated pre-HPCL scaffold with homogeneous hydrophilic characteristics all over the material. Consequently, the cells could proliferate well, infiltrate most deeply and ultimately produce the highest amounts of the cartilage-specific substances throughout this scaffold.

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Surface hydrolysis of fibrous poly(ε-caprolactone) scaffolds for enhanced osteoblast adhesion and proliferation

Cited by 68 publications

References 26 publications

Influence of random and oriented electrospun fibrous poly(lactic‐co‐glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

Influence of random and oriented electrospun fibrous poly(lactic‐co‐glycolic acid) scaffolds on neural differentiation of mouse embryonic stem cells

Fabrication of endothelial cell-specific polyurethane surfaces co-immobilized with GRGDS and YIGSR peptides

Enhancement of chondrocyte proliferation, distribution, and functions within polycaprolactone scaffolds by surface treatments

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