Surface glycoproteins and concanavalin‐A‐mediated agglutinability of clonal variants and tumour cells derived from SV40‐virus‐transformed mouse 3T3 cells

Smets, L. A.; Beek, Wim P. Van; Rooij, H. Van

doi:10.1002/ijc.2910180411

Cited by 41 publications

(10 citation statements)

References 16 publications

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“…Bordelon [24], Shields [8] reported that there was no corre lation between this test and the transformed phenotype. It is nevertheless of value in confirming previously obtained results [7,19] or in certain cell systems [25]. In the same way, the SD1 and A9/SEWA hybrid cell lines, derived from the common SEWA parent, show very similar agglutination ability to Con A, a similar ability to form colonies in soft agar and the same saturation density.…”

Section: Discussionsupporting

confidence: 85%

Correlation between Characteristics of Transformation and Malignancy of Intra and Interspecific Somatic Hybrid Cells

C¹,

Simonetti²,

Berebbi³

et al. 1978

Oncology

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The transformed properties of five hybrid cell lines which had either one or another parent in common were studied and compared with their tumorigenicity. Three hybrid cell lines, derived from the Chinese hamster DC-3F/ADX/Aza line, were resistant to actinomycin-D. This property seemed to be correlated with the presence of a marker chromosome from the common parent. The tumorigenicity was intermediate between those of the parent cell lines. On the other hand, agglutinability by concanavalin A (Con A) was variable. Three hybrid cell lines which had either the A9 or the clone 1D (both derived from mouse fibroblasts) showed very similar transformed characteristics, but two were tumorigenic and one not so. It appears from this study that the properties of the hybrid cell lines can be influenced more by one parent, depending on the genes retained at chromosome segregation. The limits of Con A agglutination as a characteristic of transformation and the validity of the check pouch grafts as tumorigenicity test for malignant human cell lines are discussed.

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Section: Discussionsupporting

confidence: 85%

Correlation between Characteristics of Transformation and Malignancy of Intra and Interspecific Somatic Hybrid Cells

C¹,

Simonetti²,

Berebbi³

et al. 1978

Oncology

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“…The solid tumors originating from the transfectants were explanted in vitro using collagenase, hyaluronidase and pronase (Smets et al, 1976). Tumor cells were cultured in vitro for further characterization.…”

Section: Test For Tumorigenicitymentioning

confidence: 99%

“…The tumorigenicity of tumor-DNA-induced transfectants was tested by S.C. inoculation of 500,000 cells in the posterior side of the neck of 2 to 3-month-old c3H nude mice. The solid tumors originating from the transfectants were explanted in vitro using collagenase, hyaluronidase and pronase (Smets et al, 1976). Tumor cells were cultured in vitro for further characterization.…”

Section: Test For Tumorigenicitymentioning

confidence: 99%

Transfection by human oncogenes: Concomitant induction of tumorigenicity and tumor‐associated membrane alterations

Collard

Beek

Janssen

et al. 1985

Intl Journal of Cancer

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NIH 3T3 cells were transfected with DNA derived from human bladder carcinoma, colon carcinoma and HL60 promyelocytic leukemia cells. The transfectants were examined for the presence of human oncogenes in relation to tumorigenic potential and composition of surface-located fucosyl glycopeptides by gel filtration, Concanavalin-A-binding and high-performance liquid chromatography. All transfectants, harboring 3 different human cellular ras genes, appeared to be tumorigenic in nude mice and displayed characteristically altered glycopeptides. The surface glycopeptides were consistently changed to higher apparent molecular weight due to enrichment in higher-branched sialic-acid-containing glycopeptides. Similar alterations have been found previously in virally- and chemically-transformed cells in vitro and tumors raised in vivo, and were designated as cancer-related or tumor-associated glycopeptides. Revertants derived from HL60-DNA-induced transfectants, which had lost the transfected human N-ras oncogene, simultaneously lost their tumorigenic potential and expression of cancer-related membrane glycopeptides. In addition, spontaneous transformants, exhibiting morphology and growth patterns indistinguishable from those of tumor-DNA-induced transfectants, neither contained transferred human DNA sequences nor expressed cancer-related glycopeptides. Nevertheless these cells were capable, after prolonged latency periods, of inducing tumors in nude mice. Cells derived from such tumors constantly displayed cancer-related glycopeptides on their surface, suggesting selection of tumorigenic cells from spontaneous transformants during passage in nude mice. In one of these tumors at least, an endogenous mouse ras-gene appeared to be activated. The results indicate a close correlation between the presence of activated ras-oncogenes in the genome of the transfected cells, the tumorigenic potential of these cells and the expression of surface-located cancer-related glycopeptides. The data suggest that functions provided by human ras-oncogenes contribute to the alteration of membrane glycopeptides on tumor cells.

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“…However, more extended studies have recently shown that these phenomena are 11 not, or are not generally, characteristic of tumourigenic cells (Kolata, 1975;Shin et al, 1975;Hynes, 1976;Nicolson, 1976;Shields, 1976;Smets, Van Beek and Van Rooij, 1976;Nilsson et al, 1977). These findings underline the importance of extending investigations to several cell systems.…”

mentioning

confidence: 99%

Comparison of cell-surface glycoproteins of rat hepatomas and embryonic rat liver

Beek¹,

Emmelot²,

Homburg³

1977

Br J Cancer

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Summary.-Cell-surface glycoprotein of 3 rat hepatoma strains and late-embryonic liver was metabolically labelled in vivo with [3H]-or [14C]-fucose. Trypsinization of the cells and exhaustive pronase digestion of combined hepatoma-liver trypsinates followed by gel filtration over Sephadex-Biogel mixtures, yielded elution profiles that contained more early-eluting (high-mol.-wt.) glycopeptides for hepatomas than for liver. At least 3 factors were identified which acted to augment the fraction of earlyeluting tumour glycopeptides: (a) increase of neuraminidase-sensitive sialic acid, (b) increase of neuraminidase -insensitive sialic acid that was sensitive to mild HCI hydrolysis, and (c) presence of sugar sulphate groups contributing to a restricted extent, relative to possible unknown factor(s). Whether (a), (b) or (c) operated depended on the hepatoma strain or its mode of growth. Notwithstanding these differences in the nature of the increase in early-eluting glycopeptides, the increase itself appears not to be due to growth per se, nor to an embryonic expression, but rather may serve as a marker of tumourigenicity.

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Surface glycoproteins and concanavalin‐A‐mediated agglutinability of clonal variants and tumour cells derived from SV40‐virus‐transformed mouse 3T3 cells

Cited by 41 publications

References 16 publications

Correlation between Characteristics of Transformation and Malignancy of Intra and Interspecific Somatic Hybrid Cells

Correlation between Characteristics of Transformation and Malignancy of Intra and Interspecific Somatic Hybrid Cells

Transfection by human oncogenes: Concomitant induction of tumorigenicity and tumor‐associated membrane alterations

Comparison of cell-surface glycoproteins of rat hepatomas and embryonic rat liver

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