Surface Functionalized Anodic Aluminum Oxide Membrane for Opto-Nanofluidic SARS-CoV-2 Genomic Target Detection

Abstract

An ultra-thin and highly sensitive SARS-CoV-2 detection platform was demonstrated using a nano-porous anodic aluminum oxide (AAO) membrane. The membrane surface was functionalized to enable efficient trapping and identification of SARS-CoV-2 genomic targets through DNA-DNA and DNA-RNA hybridization. To immobilize the probe oligonucleotides on the AAO membrane, the pore surface was first coated with the linking reagents, 3-aminopropyltrimethoxysilane (APTMS) and glutaraldehyde (GA), by a compact vacuum infiltration module. After that, complementary target oligos with fluorescent modifier was pulled and infiltrated into the nano-fluidic channels formed by the AAO pores. The fluorescent signal applying the AAO membrane sensors was two orders stronger than a flat glass template. In addition, the dependence between the nano-pore size and the fluorescent intensity was evaluated. The optimized pore diameter d is 200 nm, which can accommodate the assembled oligonucleotide and aminosilane layers without blocking the AAO nano-fluidic channels. Our DNA functionalized membrane sensor is an accurate and high throughput platform supporting rapid virus tests, which is critical for population-wide diagnostic applications result in a page being rejected by search engines.

Array of Nanopore Sensors Detect Nanoscale Biomolecules by Nucleic Acid Analysis

“…An AAO membrane with opto-nanofluidics can be used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). [79] The surface functionalization process followed by 3aminopropyltrimethoxysillane (APTMS), glutaraldehyde (GA) and the probe DNA (pDNA)through the layer-by-layer. Then, SARS-CoV-2 tDNA target analytes were applied to the nanoporous surface based on the DNA-DNA hybridization method.…”

“…In the winter of 2019, COVID‐19 became one of the most critical public health challenges in recent history. An AAO membrane with opto‐nanofluidics can be used to detect severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) [79] . The surface functionalization process followed by 3‐aminopropyltrimethoxysillane (APTMS), glutaraldehyde (GA) and the probe DNA (pDNA)through the layer‐by‐layer.…”

Nanoscale self-assembly: concepts, applications and challenges

“…In addition, this technique was also reported to be used for detecting other categories of SARS-CoV-2 respiratory viruses and to monitor cases of nucleic acid sequence mutations. Future porous nanomaterials that could find application for next-generation sequencing or biosensing include carbon nanostructures [558] and AAO membranes [559].…”

Organic–inorganic hybrid self-pigmenting effect of anodic aluminium oxide membranes

“…In eqn (7), e b = 1, and e a = 1.834 2 at 254 nm. 60 If we replace the absorption coefficient a i with an extinction coefficient k i (k i = log e a i ), 61 eqn (2) can also be expressed as…”

“…Self-ordered anodic aluminium oxide (AAO) membranes with hexagonally packed nanochannel arrays have become promising candidates for versatile applications including nanofluidic transport, 1 filtration, 2 separation, 3 ionic current rectification, 4 sensors, 5,6 and biodetection, 7 as well as the construction of various nanostructures (such as nanorods, 8,9 nanowires, 10,11 nanoparticles 12 and nanopillars 13 ), and the design of optical devices, 13 biosensor 14,15 and energy conversion devices 16 through the AAO template-based assembly approach, where the composition and the structural characteristics of the AAO membranes play key roles in their functional applications. Interestingly, the compositions of the AAO membranes are not completely composed of aluminium oxide; partially acid anion-contaminated species have been inevitably incorporated into the membrane during the formation of the nanochannels, 17,18 and the alumina composition in the barrier layer produces a different pattern of surrounding hexagonal walls.…”