Surface Functionalization of Titanium with Chitosan/Gelatin via Electrophoretic Deposition: Characterization and Cell Behavior

Abstract

The electrophoretic deposition (EPD) is a versatile and cost-effective technique for fabricating advanced coatings. In this study, chitosan/gelatin (CS/G) coatings were prepared on titanium substrates via EPD. The prepared coatings were characterized using fluorescence microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and shear bond strength testing. It was found that CS/G coatings had a similar macroporous structure. The gelatin content in the CS/G coatings gradually increased with the increase of the gelatin in the blend solutions. The shear bond strength of the CS/G coatings also increased with the increasing gelatin content. In vitro biological tests demonstrated that human MG63 osteoblast-like cells achieved better affinity on the coatings with higher gelatin content. Therefore, it was concluded that EPD was an effective and efficient technique to prepare CS/G coatings on the titanium surface and that CS/G coatings with higher gelatin content were promising candidates for further loading of functional agents.

A green single-step procedure to synthesize Ag-containing nanocomposite coatings with low cytotoxicity and efficient antibacterial properties

“…Our previous study showed that such properties and the bond strength of the CS coatings could be enhanced by the addition of gelatin. 31,32 The results herein demonstrate that this type of Ag-containing coating fabricated on a metal substrate via green chemistry could be efficient in killing bacteria while showing relatively low cytotoxicity toward MC3T3-E1 cells compared with Ag ions.…”

“…31 Briefly, 1.2 g of chitosan and 2.8 g of gelatin were Preparation of the titanium substrates and ePD process…”

Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings

“…25 Briefly, the disks were sandblasted with large grits and acid etched with HCl/H 2 SO 4 for 30 minutes at 60°C, and then ultrasonically cleaned in acetone, ethanol, and Milli-Q water sequentially. Chitosan (MW 1,000,000 Da) with a deacetylation degree .95% was obtained from Golden-Shell Biochemical Co, Ltd (Zhejiang, China).…”

“…25 In brief, 0.6 g chitosan was dissolved in 80 mL 0.04 M hydrochloric acid with magnetic stirring at room temperature for 2 hours. The pH value was adjusted to 4.0 using 0.5 M NaOH; then, 1.4 g gelatin was dissolved into the chitosan solution at 60°C for 1.5 hours, and the total volume of the solution was brought to 100 mL with Milli-Q water.…”

“…23,24 In our previous studies, EPD technique was routinely used to prepare organic chitosan-gelatin (CSG) nanosphere coating on Ti substrates. [25][26][27] It showed favorable mechanical property, swelling ability, biodegradability, and biocompatibility. Moreover, the mild coating condition (room temperature, water solution) and convenient protocol make it a promising candidate for further loading of functional agents like metal ions.…”