Surface functionalization of anticoagulation and anti-nonspecific adsorption with recombinant hirudin modification

Zheng, Zhiwen; Li, Xueyang; Dai, Xin; Ge, Jianhui; Chen, Yunhua; Du, Chang

doi:10.1016/j.bioadv.2022.212741

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…20 Polycaprolactone (PCL) is the most popular, widely used, biodegradable, biocompatible, chemically and thermally stable, used as drug carrier, permeable and excellent electro spinnable at low voltages, and give good strength to the matrix. 21,22 It is extensively used in wound healing therapy as it is approved by Food and Drug Administration Organization to be used for wound dressing application because of its outstanding biological properties. Nevertheless, PCL is hydrophobic that affects cell adhesion, cell growth, cell proliferation, and migration negatively.…”

Section: Introductionmentioning

confidence: 99%

Indigenous Ethiopian traditional medicinal plant leaf extract, Croton macrostachyus, loaded PCL electrospun nanofibrous mat as potential wound dressing: In vitro analysis

Biruk Fentahun,

Jing,

Xiangnan

et al. 2024

Journal of Bioactive and Compatible Polymers

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The main aim of this study was to investigate the potential application of an indiginous Ethiopian medicinal plant, Croton macrostachyus, into PCL nanofibers through electrospinning for the first time. Croton macrostachyus (CM) leaf has antibacterial and wound healing properties. Croton macrostachyus of 1%, 3%, 5%, and 7% (w/v) incorporated PCL nanofibrous mats were produced through electrospinning. The produced nanofibrous mats morphology observed by scanning electron microscopy (SEM) was found to be continuous, bead-free, and interconnected. The average diameters of PCL, PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofibrous mat is 309 ± 114, 271 ± 66, 235 ± 56, 226 ± 65, and 216 ± 42 nm, respectively. According to the FTIR results, the CM leaf extract was successfully incorporated into the PCL nanofibers. The bacterial reduction percentage value of PCL-1%CM, PCL-3%CM, PCL-5%CM, and PCL-7%CM nanofiber mats against S. aureus is 77.00%, 99.88%, 99.91%, and 99.99%, and 35.00%, 78.00%, 90.00%, and 99.30% against E. coli. The in vitro release study showed that there was an immediate release of CM within 24 h and then a steady sustained release of 51.36, 53.92, 54.99, and 57.12% up to 72 h at 1%, 3%, 5%, and 7% concentration, respectively. The presence of CM leaf extract in the PCL nanofiber increased the hydrophilicity of the pure PCL nanofiber (100% hydrophilic at 7% leaf extract concentration). In general, the results of the in vitro study confirmed that the PCL nanofibrous mats loaded with CM leaf extract are suitable for use as an effective wound dressing with a broad spectrum of antimicrobial activity and hydrophilic properties.

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Section: Introductionmentioning

confidence: 99%

Indigenous Ethiopian traditional medicinal plant leaf extract, Croton macrostachyus, loaded PCL electrospun nanofibrous mat as potential wound dressing: In vitro analysis

Biruk Fentahun,

Jing,

Xiangnan

et al. 2024

Journal of Bioactive and Compatible Polymers

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Layer‐by‐Layer Construction of Antibacterial and Anticoagulant Blood Contacting Materials

Zheng,

Li,

Dai

et al. 2024

Macromolecular Bioscience

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Vascular transplantation is a common treatment for Cardiovascular disease (CVD). However, the mismatch of mechanical, structural, or microenvironmental properties of materials limits the clinical application. Therefore, the functional construction of artificial vessels or other blood contact materials remains an urgent challenge. In this paper, the composite nanofibers of polycaprolactone (PCL) with dopamine and polyethylenimine (PEI) coating were firstly prepared, which were further self‐assembled by anticoagulant hirudin (rH) and antimicrobial peptide (AMP) of HHC36 through layer‐by‐layer (LBL) method. The results of FTIR and XPS analysis showed that hirudin and AMP were successfully loaded on PEI‐PDA/PCL nanofibers and the hydrophilicity was improved. They also showed good mechanical properties that the ultimate tensile strength and elongation at break were better than natural blood vessels. The antibacterial results showed that the antibacterial effect was still 93% against E. coli on the 5th day because of the stable and continuous release of AMP and rH. The performance of anticoagulant activity also exhibited the same results, which APTT was even 9.7s longer in the experimental group than the control group on the 5th day. The novel materials would be effectively solve the formation of thrombosis around artificial blood vessel grafts and the treatment of inflammation.This article is protected by copyright. All rights reserved

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PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

Zheng

Dai

et al. 2023

Chem. Res. Chin. Univ.

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Surface functionalization of anticoagulation and anti-nonspecific adsorption with recombinant hirudin modification

Cited by 4 publications

References 31 publications

Indigenous Ethiopian traditional medicinal plant leaf extract, Croton macrostachyus, loaded PCL electrospun nanofibrous mat as potential wound dressing: In vitro analysis

Indigenous Ethiopian traditional medicinal plant leaf extract, Croton macrostachyus, loaded PCL electrospun nanofibrous mat as potential wound dressing: In vitro analysis

Layer‐by‐Layer Construction of Antibacterial and Anticoagulant Blood Contacting Materials

PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

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