Surface-enhanced Raman spectroscopy (SERS) for monitoring colistin-resistant and susceptible E. coli strains

Mushtaq, Aqsa; Nawaz, Haq; Majeed, Muhammad Irfan; Rashid, Nosheen; Tahir, Muhammad; Nawaz, M.; Shahzad, Kashif; Dastgir, Ghulam; Bari, Rana Zaki Abdul; Haq, Anwar Ul; Saleem, Muhammad Abdullah; Akhtar, Farwa

doi:10.1016/j.saa.2022.121315

Cited by 15 publications

(7 citation statements)

References 73 publications

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“…In another similar study conducted by Mushtaq et al, PCA and PLS-DA were used to analyse data obtained from SERS using silver nanoparticles as substrates towards differentiation of colistin resistant and susceptible E. coli. [81] The PCA scatter plot of the SERS spectral data sets showed that the spectra of resistant strain formed clusters on the negative side while those of the susceptible strain formed cluster on the positive side of the scatter plot. The first and second principal component described 67.67 % and 14.69 % variances, respectively.…”

Section: Chemometrics In Detection Of E Colimentioning

confidence: 98%

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

et al. 2023

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Escherichia coli is a harmful pathogenic bacterial species causing serious intestinal sickness in humans. This bacterium has been linked to contaminated epidemics that have led to significant mortality and morbidity across the world. E. coli, like most other waterborne infections, is tricky to discover effectively in the water supply. Therefore, there is a demand for advanced E. coli detection methods that can sensitively and rapidly detect these pathogens. This review reveals several approaches used for the detection of E. coli bacteria using conventional methods such as multiple tube fermentation and membrane filtration techniques. The emerging approaches give quite accurate and speedy identification despite the necessity for culturing; nevertheless, they lack precision and necessitate extra lab testing. Because analytical techniques such as GC‐DMS lack specificity, the invention of a sensing device that is simple to use, compact, extremely sensitive, and specific has proven essential in identifying incredibly low concentrations of harmful E. coli in drinking water.

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Section: Chemometrics In Detection Of E Colimentioning

confidence: 98%

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

et al. 2023

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Section: Analysis Of Bacteria Antibiotic Resistance and Susceptibilitymentioning

confidence: 99%

“…SERS has been successfully applied to analyze the different responses of antibiotic resistant and sensitive bacteria during exposure to antibiotics to assess the antibiotic susceptibility of bacteria. 80 It has been applied to differentiate the antibiotic-resistant and susceptible E. coli strains, [81][82][83] S. Typhimurium, 84 B. cereus, 82,85 S. aureus, 86 and carbapenem sensitive and resistant Klebsiella strains from clinical samples. 87 In addition to the instant one-time treatment of antibiotics, the antibiotic susceptibility testing was conducted over a long period such as three months, or 21 days in some studies, to investigate the dynamic molecular changes in the drug-resistant strains.…”

Section: Different Bacterial Responses During Antibiotics Exposurementioning

confidence: 99%

“…The treatment of antibiotics can amplify their differences because of their varied responses to antibiotics. The methods used to analyze the Raman spectra for antibiotic resistance studies include chemometrics methods such as PCA and PLS-DA, [81][82][83][84]89,90 and machine learning algorithms such as CNN and SVM. 87,90,91 SERS or Raman spectroscopy for the analysis of bacteria antibiotic resistance and susceptibility with different data processing methods was shown in Figure 4.…”

Section: Different Bacterial Responses During Antibiotics Exposurementioning

confidence: 99%

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Recent advances of Raman spectroscopy for the analysis of bacteria

Rodriguez

Zhang

Wang

2023

Analytical Science Advances

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Rapid and sensitive bacteria detection and identification are becoming increasingly important for a wide range of areas including the control of food safety, the prevention of infectious diseases, and environmental monitoring. Raman spectroscopy is an emerging technology which provides comprehensive information for the analysis of bacteria in a short time and with high sensitivity. Raman spectroscopy offers many advantages including relatively simple operation, non‐destructive analysis, and information on molecular differences between bacteria species and strains. A variety of biochemical properties can be measured in a single spectrum. This short review covers the recent advancements and applications of Raman spectroscopy for bacteria analysis with specific focuses on bacteria detection, bacteria identification and discrimination, as well as bacteria antibiotic susceptibility testing in 2022. The development of novel substrates, the combination with other techniques, and the utilization of advanced data processing tools for the improvement of Raman spectroscopy and future directions are discussed.

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“…Colistin resistant Klebsiella pneumoniae was discriminated using SERS-based (Surface-enhanced Raman spectroscopy) biosensor coupled with machine learning techniques [ 26 ]. A recent study reported the feasibility of discrimination between sensitive and colistin resistant E. coli strains [ 27 ]. This study involves silver nanoparticle based SERS measurements to delineate spectral signals specific for colistin resistance and explored the feasibility of classification using machine learning.…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy

et al. 2022

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The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance.

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Surface-enhanced Raman spectroscopy (SERS) for monitoring colistin-resistant and susceptible E. coli strains

Cited by 15 publications

References 73 publications

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

Approaches for the Detection of Escherichia coli in Wastewater: A Short Review

Recent advances of Raman spectroscopy for the analysis of bacteria

Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy

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