Surface‐enhanced Raman spectroscopy of DNA bases

Otto, Cornelis; Tweel, T.J.J. van den; Mul, F.F.M. de; Greve, Jan

doi:10.1002/jrs.1250170311

Cited by 245 publications

(264 citation statements)

References 25 publications

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“…4 are normalised with respect to the strongest Raman band in each spectrum. The spectra are in good agreement with previous reports, 9,14,22 and demonstrate that a straightforward identification of the bases is possible. The detailed assignments of the Raman bands for the bases have already been established by Giese and McNaughton.…”

Section: Resultssupporting

confidence: 81%

Surface‐ and tip‐enhanced Raman scattering of DNA components

Rasmussen

Deckert

2006

J Raman Spectroscopy

184

159

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While surface-enhanced Raman scattering (SERS) is an established technique to significantly enhance the Raman signal from samples bound to metal surfaces, a quantitative interpretation is very difficult because of the rough and arbitrary nature of the substrate. A distinction between concentration and enhancement effects is virtually impossible. Tip-enhanced Raman scattering (TERS), a scattering type of near-field optical microscopy technique, circumvents the problem of random enhancement by concentrating the roughness to only a small region directly at the apex of a scanning probe tip. In this paper, TERS spectra of DNA pyrimidine bases have been collected and compared with standard SERS and Raman measurements of the nucleotides and the pure bases. The results are discussed in terms of electromagnetic and chemical effects of the SERS enhancement of the TERS probes.

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Section: Resultssupporting

confidence: 81%

Surface‐ and tip‐enhanced Raman scattering of DNA components

Rasmussen

Deckert

2006

J Raman Spectroscopy

184

159

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“…The spectral peaks in Fig. 6c are also somewhat similar to the spectra of adenosine and guanosine 24,32 , albeit with a peak shift towards the red of 20 cm À 1 .…”

Section: Nature Communications | Doi: 101038/ncomms6144mentioning

confidence: 54%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

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“…The SECARS spectrum of adenine, one of the constituent bases of DNA and RNA, is dominated by the À 740 cm À 1 ring-breathing mode 36 (Fig. 3e).…”

mentioning

confidence: 99%

Coherent anti-Stokes Raman scattering with single-molecule sensitivity using a plasmonic Fano resonance

et al. 2014

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Plasmonic nanostructures are of particular interest as substrates for the spectroscopic detection and identification of individual molecules. Single-molecule sensitivity Raman detection has been achieved by combining resonant molecular excitation with large electromagnetic field enhancements experienced by a molecule associated with an interparticle junction. Detection of molecules with extremely small Raman cross-sections (B10 À 30 cm 2 sr À 1 ), however, has remained elusive. Here we show that coherent anti-Stokes Raman spectroscopy (CARS), a nonlinear spectroscopy of great utility and potential for molecular sensing, can be used to obtain single-molecule detection sensitivity, by exploiting the unique light harvesting properties of plasmonic Fano resonances. The CARS signal is enhanced by B11 orders of magnitude relative to spontaneous Raman scattering, enabling the detection of single molecules, which is verified using a statistically rigorous bi-analyte method. This approach combines unprecedented single-molecule spectral sensitivity with plasmonic substrates that can be fabricated using top-down lithographic strategies.

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Surface‐enhanced Raman spectroscopy of DNA bases

Cited by 245 publications

References 25 publications

Surface‐ and tip‐enhanced Raman scattering of DNA components

Surface‐ and tip‐enhanced Raman scattering of DNA components

Laser-targeted photofabrication of gold nanoparticles inside cells

Coherent anti-Stokes Raman scattering with single-molecule sensitivity using a plasmonic Fano resonance

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