Surface-Enhanced Raman Spectroscopy Detection of Cerebrospinal Fluid Glucose Based on the Optofluidic In-Fiber-Integrated Composites of Graphene Oxide, Silver Nanoparticles, and 4-Mercaptophenylboronic Acid

Abstract

In this work, a self-assembled surface-enhanced Raman scattering (SERS) sensor based on an optofluidic microstructured hollow fiber (MHF) integrated composites of graphene oxide (GO), silver nanoparticles (Ag NPs), and 4mercaptophenylboronic acid (4-MPBA) as a glucose detection device was proposed. This SERS substrate is specific to glucose and grows on the outside of the core of MHF. Here, the MHF has a microchannel, where a suspended core was attached. In the microchannel, the trace sample solution was interacted with the SERS substrate, realizing the Raman online sensing detection of trace cerebrospinal fluid glucose. In the range of 0.5−10 mmol/L, the results present that detection of the glucose concentration shows good linearity without external interference. Meanwhile, this type of rapid, online, and label-free SERS sensor of glucose shows the ability to detect excessive glucose content in the actual cerebrospinal fluid environment of the Sprague−Dawley rats, providing a simple detection method for meningitis, meningeal multiple malignant tumor metastasis, and related diseases caused by abnormal glucose content in cerebrospinal fluid.

Ag Nanoparticle-Decorated Graphene Oxide Coatings on the Inner Walls of Optofluidic Capillaries for Real-Time Trace SERS Detection

“…Optofluidic SERS platforms, such as capillaries or microstructure optical fibers, have been extensively studied to confine and transmit light along the fiber length for large SERS signal accumulation. The inherent fluidic channels are suitable for integrating chemical affinity function and have rarely been studied. , …”

“…The inherent fluidic channels are suitable for integrating chemical affinity function and have rarely been studied. 14,15 In this paper, we propose a capillary coated with GO/Ag nanoparticle composites (GO/Ag) as an ultrasensitive SERS substrate. GO has good water dispersibility and affinity with aromatic compounds.…”

A Dual-Mode NADH Biosensor Based on Gold Nanostars Decorated CoFe2 Metal–Organic Frameworks to Reveal Dynamics of Cell Metabolism

“…When the NADH solution incubates with MPBA-Au/CoFe 2 -MOFs, the Raman intensity at 1076 cm –1 first increases and then remains constant after 60 min (Figure D). This phenomenon indicates that NADH molecules can specifically react to the boronic acid groups in MPBA, increasing the SERS intensity of MPBA under charge-transfer effects, and the action of orientation changes . Then the Raman intensity reached a platform after 60 min, indicating complete hybridization.…”

“…This phenomenon indicates that NADH molecules can specifically react to the boronic acid groups in MPBA, increasing the SERS intensity of MPBA under charge-transfer effects, and the action of orientation changes. 33 Then the Raman intensity reached a platform after 60 min, indicating complete hybridization. The intensity−time curve is shown in Figure 3E, which is in line with the result in Figure 3D.…”

Comparison of Electrocatalytic Performance of CuCo2O4 Nanorods and Nanospheres Decorated with Co3S4 Nanosheets for Electrochemical Sensing of Hydrogen Peroxide and Glucose in Human Serum

“…Among various analytical techniques, 9,10 electrochemicalbased sensors have attracted much interest due to their capability to satisfy the abovementioned requirements. 11−14 This is due to their advantages of simplicity, high sensitivity, low cost, fast operation, high throughput, and capability of miniaturization.…”