Surface-Enhanced Raman Spectroscopy-Based Sandwich Immunoassays for Multiplexed Detection of Zika and Dengue Viral Biomarkers

Sánchez-Purrà, Maria; Carré-Camps, Marc; Puig, Helena de; Bosch, Irene; Gehrke, Lee; Hamad‐Schifferli, Kimberly

doi:10.1021/acsinfecdis.7b00110

Cited by 147 publications

(106 citation statements)

References 52 publications

(81 reference statements)

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“…Schifferli et al reported a gold nanostar (Au NS)-based LFA platform (Fig. 14), with the SERS limit of detection of 0.72 ng/mL of ZIKV NS1 and 7.67 ng/mL of DENV NS1 [192]. Nano Res.…”

Section: Plasmonic Paper Devicesmentioning

confidence: 99%

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Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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Plasmonics-based biosensing assays have been extensively employed for biomedical applications. Significant advancements in use of plasmonic assays for the construction of point-of-care (POC) diagnostic methods have been made to provide effective and urgent health care of patients, especially in resourcelimited settings. This rapidly progressive research area, centered on the unique surface plasmon resonance (SPR) properties of metallic nanostructures with exceptional absorption and scattering abilities, has greatly facilitated the development of cost-effective, sensitive, and rapid strategies for disease diagnostics and improving patient healthcare in both developed and developing worlds. This review highlights the recent advances and applications of plasmonic technologies for highly sensitive protein and nucleic acid biomarker detection. In particular, we focus on the implementation and penetration of various plasmonic technologies in conventional molecular diagnostic assays, and discuss how such modification has resulted in simpler, faster, and more sensitive alternatives that are suited for point-of-use. Finally, integration of plasmonic molecular assays with various portable POC platforms for mobile health applications are highlighted.

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“…Schifferli et al reported a gold nanostar (Au NS)-based LFA platform (Fig. 14), with the SERS limit of detection of 0.72 ng/mL of ZIKV NS1 and 7.67 ng/mL of DENV NS1 [192]. Nano Res.…”

Section: Plasmonic Paper Devicesmentioning

confidence: 99%

“…2018, 11(10): 5439-5473 Reproduced with permission from Ref. [192], © American Chemical Society 2017.…”

Section: Plasmonic Paper Devicesmentioning

confidence: 99%

Plasmonic molecular assays: Recent advances and applications for mobile health

Wei

2018

Nano Res.

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“…The assay run time was optimized to 9 min and 20 min (Table 1) including sample preparation and loading. Until now the Zika NS1 levels in Zika-infected patients were still largely unknown/variable, whereas approximately 15 μg/mL Dengue NS1 can be found for Dengue-infected patient's serum after two days of infection [12,22]. Based on that, we have analyzed 2000.0, 1000.0, 500.0, 250.0, 125.0, and 62.5 ng/mL Zika NS1 spiking on plasma to perform M-ELISA on-chip (Figure 3b).…”

Section: Resultsmentioning

confidence: 99%

“…IgG and IgM antibodies are produced in the human body at the later stage of the infection (four to seven days), this makes them inapt for early stage detection. In contrast, NS1 antigen with a similar structure has been considered for highly sensitive, specific early stage detection for different flaviviruses previously [9][10][11][12][13][14]. There are currently numerous POC devices that detect ZIKV by using NS1 as a biomarker.…”

Section: Introductionmentioning

confidence: 99%

Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

Kabir

Zilouchian²,

Sher

et al. 2020

Diagnostics

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The Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitoes that can potentially cause microcephaly, Guillain–Barré Syndrome, and other birth defects. Effective vaccines for Zika have not yet been developed. There is a necessity to establish an easily deployable, high-throughput, low-cost, and disposable point-of-care (POC) diagnostic platform for ZIKV infections. We report here an automated magnetic actuation platform suitable for a POC microfluidic sandwich enzyme-linked immunosorbent assay (ELISA) using antibody-coated superparamagnetic beads. The smartphone integrated immunoassay is developed for colorimetric detection of ZIKV nonstructural protein 1 (NS1) antigen using disposable chips to accommodate the reactions inside the chip in microliter volumes. An in-house-built magnetic actuator platform automatically moves the magnetic beads through different aqueous phases. The assay requires a total of 9 min to automatically control the post-capture washing, horseradish peroxidase (HRP) conjugated secondary antibody probing, washing again, and, finally, color development. By measuring the saturation intensity of the developed color from the smartphone captured video, the presented assay provides high sensitivity with a detection limit of 62.5 ng/mL in whole plasma. These results advocate a great promise that the platform would be useful for the POC diagnosis of Zika virus infection in patients and can be used in resource-limited settings.

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“…The buffer solution was 1 l of 1x PBS with 1 mM MgCl2. The Raman peak due to Zika was around 1590 cm -1 [34] and [35].…”

Section: (B) One Could Resolve Individual Zika Viruses On Goldmentioning

confidence: 99%

Whole Virus Detection using Paper Potentiometry

Tabib-Azar¹,

Dolai²

2020

Preprint

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Paper-based sensors, microfluidic platforms and electronic devices have attracted attention in the past couple of decades because they are flexible, can be recycled easily, environmentally friendly, and inexpensive. Here we report a paper aptamer-based potentiometric sensor to detect the whole Zika virus for the first time with a minimum sensitivity of 2.6 nV/Zika and the minimum detectable signal (MDS) of 0.8x1e6 Zika. Our paper sensor works very similar to a P-N junction where a junction is formed between two different wet regions with different electrochemical potentials near each other on the paper. These two regions with slightly different ionic contents, ionic species and concentrations, produce a potential difference given by the Nernst equation. Our paper sensor consisted of a 2-3 mm x 10 mm segments of a paper with a conducting silver paint contact patches on its two ends. The paper is soaked in a buffer solution containing aptamers designed to bind to the capsid proteins on Zika. Atomic force microscopy studies were carried out to show both the aptamer and Zika become immobilized in the paper. We then added the Zika (in its own buffer or simulant Urine) to the region close to one of the silver-paint contacts. The Zika virus (40 nm diameter with 43 kDa or 7.1x10-20 gm weight), became immobilized in the paper’s pores and bonded with the resident aptamers creating a concentration gradient. The potential measured between the two silver paint contacts reproducibly became more negative as upon adding the Zika. We also showed that an LCD powered by the sensor, can be used to detect the sensor output.

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Surface-Enhanced Raman Spectroscopy-Based Sandwich Immunoassays for Multiplexed Detection of Zika and Dengue Viral Biomarkers

Cited by 147 publications

References 52 publications

Plasmonic molecular assays: Recent advances and applications for mobile health

Plasmonic molecular assays: Recent advances and applications for mobile health

Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

Whole Virus Detection using Paper Potentiometry

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