Surface‐Enhanced Raman Scattering Study of Silver Nanoparticles Prepared by Using MC as a Template

Abstract

A good Ag-based SERS substrate has been prepared by one-pot reaction using methyl cellulose as a template. Effects of methyl cellulose concentration, silver ammonia chloride solution concentration, reaction duration, and reducing agent on silver nanoparticles were discussed in this paper. The performance of the obtained Ag nanoparticles was characterized by UV-visible spectroscopy, transmission electron microscopy, and surface-enhanced Raman spectroscopy. Results show that the reducing agent plays a crucial role in the performance of silver nanoparticles. Optimum preparation conditions of synthesis of SERS substrates were as follows: 10 mM silver ammonia chloride and 0.2% MC at 75°C, reducing in 0.2% reducing agent at 120 min. TEM studies reveal that particles are mostly spherical and rod in shape with an average size of 80 nm. Silver nanoparticles prepared with MC as a template have been shown to provide strong SERS enhancement signals of R6G, which can be used as a good Ag-based SERS substrate in the analytical environment for routine measurements.

Recent developments in multiplexing techniques for immunohistochemistry

“…Currently, multiplex detection of up to 10 commercial nanotags has been achieved in a mouse model [69]. SERS is highly specific [70], and careful selection of reducing agents can lead up to a 10 5 -times amplification of signal, increasing SERS potential in use of multiplexed IHC [71]. …”

Bioreduction of precious and heavy metals by Candida species under oxidative stress conditions

“…Raman spectroscopy helps to identify molecules through spectral information, which is considered a molecular fingerprint. This technique has been widely used for the identification of NPs of different chemical composition (Lu et al ., ; Chen et al ., ). Raman spectroscopy, as observed in Table , when used for the characterization of AuNPs, revealed two peaks at 589 and 1102 nm wavelengths, which are close to those previously reported for gold nanospheres (Kalmodia et al ., ).…”

“…Raman spectroscopy, as observed in Table , when used for the characterization of AuNPs, revealed two peaks at 589 and 1102 nm wavelengths, which are close to those previously reported for gold nanospheres (Kalmodia et al ., ). For AgNPs, three peaks at 437, 1594 and 1646 nm were identified, corresponding to values essentially similar to those found for silver nanoparticles (Lu et al ., ; Tu and Chung, ). These results strongly indicate that Candida species are able to synthesize AgNPs.…”

“…These procedures make use of a large diversity of microorganisms such as bacteria, archae and yeasts (Kelly et al, 1979;Hutchins et al, 1986;Norris and Parrott, 1986;Wiegel and Ljungdahl, 1986;Biryuzova et al, 1987;Kelly and Harrison, 1989;Rawlings and Kusano, 1994;Clark and Norris, 1996;Karamushka and Gadd, 1999;Norris et al, 2000;Brandl et al, 2001;Vera et al, 2013;Madrigal-Arias et al, 2015) resulting in the isolation of macroscopic forms of minerals. Contrary to macroscopic metals, nanoparticles (NPs) exhibit physical and chemical characteristics such as optical, electrical, magnetic, colligative and catalytic properties, that depend from the form, size and method of isolation (Lu et al, 2013). Gold and silver NPs (AuNPs, AgNPs) are particularly important as some of their properties have allowed their use as therapeutic alternatives (Mandal et al, 2006;Asharani et al, 2010Asharani et al, , 2011.…”

Silver-Nanocellulose Composite Used as SERS Substrate for Detecting Carbendazim

“…Cellulose, an abundant global resource, has found use as material in many applications. Lu et al [25] used modified cellulose as a stabilizer and protective agent for an Ag colloid and obtained good SERS performance. A paper-based substrate has been popular in recent years, not only because it is inexpensive, but because of the natural wrinkles and fibril structures of paper.…”