Surface-Enhanced Raman Scattering Spectroscopy for Label-Free Analysis of P. aeruginosa Quorum Sensing

Bodelón, Gustavo; Pérez‐Juste, Jorge; Pastoriza‐Santos, Isabel

doi:10.3389/fcimb.2018.00143

Cited by 35 publications

(18 citation statements)

References 109 publications

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“…241 Copyright 2019, American Chemical Society. 150 autoinducers, 148 pyocyanin-like byproducts 243 or mitochondrial activity indicators. Much less attention has been devoted to the extracellular products released by bacteria, in the matrix surrounding the biofilms.…”

Section: Sers-based Methods For Bacterial Infection and Disease Diagnosismentioning

confidence: 99%

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

et al. 2021

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Section: Sers-based Methods For Bacterial Infection and Disease Diagnosismentioning

confidence: 99%

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

et al. 2021

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“…Furthermore, despite the progress made in the identification of the molecular spectral fingerprints (e.g. nucleobases), comprehensive databases of SERS spectra of biomolecules are still needed, as well as mathematical interpretation and processing of spectra (e.g., multivariate data analysis) [ 164 ]. Ideally, bacterial SERS biosensors should facilitate the simultaneous detection of multiple strains from complex samples.…”

Section: Microfluidics and Lab-on-a-chip Technologies Towards Rapimentioning

confidence: 99%

Rapid Methods for Antimicrobial Resistance Diagnostics

et al. 2021

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Antimicrobial resistance (AMR) is one of the most challenging threats in public health; thus, there is a growing demand for methods and technologies that enable rapid antimicrobial susceptibility testing (AST). The conventional methods and technologies addressing AMR diagnostics and AST employed in clinical microbiology are tedious, with high turnaround times (TAT), and are usually expensive. As a result, empirical antimicrobial therapies are prescribed leading to AMR spread, which in turn causes higher mortality rates and increased healthcare costs. This review describes the developments in current cutting-edge methods and technologies, organized by key enabling research domains, towards fighting the looming AMR menace by employing recent advances in AMR diagnostic tools. First, we summarize the conventional methods addressing AMR detection, surveillance, and AST. Thereafter, we examine more recent non-conventional methods and the advancements in each field, including whole genome sequencing (WGS), matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) spectrometry, Fourier transform infrared (FTIR) spectroscopy, and microfluidics technology. Following, we provide examples of commercially available diagnostic platforms for AST. Finally, perspectives on the implementation of emerging concepts towards developing paradigm-changing technologies and methodologies for AMR diagnostics are discussed.

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“…Specific marker bands and frequency regions of various biofilm constituents can also be revealed by the examination of reference samples such as microorganisms, polysaccharides, etc. 85 . MB simulated wastewater, GBs without biofilms and, the GBs after the formation of 1st and 9th weeks' old biofilm were characterized using the Raman spectroscopy (Figs.…”

Section: Evaluation Of Degradative Changes In Glass Beads (Gbs) and Imentioning

confidence: 99%

Assessment of the aerobic glass beads fixed biofilm reactor (GBs-FBR) for the treatment of simulated methylene blue wastewater

Donkadokula

Kola

Saroj

2020

Sci Rep

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The present research is focused on the application of glass beads (GBs) in fixed biofilm reactor (FBR) for the treatment of simulated methylene blue (MB) wastewater for 9 weeks under aerobic conditions. The COD of MB wastewater showed a reduction of 86.48% from 2000 to 270.4 mg/L, and BOD was declined up to 97.7% from 1095.5 to 25.03 mg/L. A drastic increase in the pH was observed until the 3rd week (8.5 to 8.28), and later, marginal changes between 8.30 ± 0.02 were noticed. A dramatic fluctuation was observed in ammonia concentration which increased (74.25 mg/L) up till the 2nd week, and from the 3rd week it started declining. In the 9th week, the ammonia concentration dropped to 16.5 mg/L. The color intensity increased significantly up till the 2nd week (259,237.46 Pt/Co) of the experiment and started decreasing slowly thereafter. The SEM–EDX analysis has shown the maximum quantity of carbon content in the GBs without biofilm, and then in the GB samples of 1st, and 9th-week old aerobic biofilms. Furthermore, Raman spectroscopy results revealed that the 9th-week GBs has a fine and strong MB peak and matched with that of the MB stock solution. Overall, the results have shown that the GBs filter media were suitable for the development of active biofilm communities for the treatment of dye wastewater. Thus, GBs-FBR system can be used for wastewater treatment to solve the current problem of industrial pollution in many countries and to protect the aquatic environment from dye pollution caused by the textile industry.

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Surface-Enhanced Raman Scattering Spectroscopy for Label-Free Analysis of P. aeruginosa Quorum Sensing

Cited by 35 publications

References 109 publications

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Surface-enhanced Raman spectroscopy for bioanalysis and diagnosis

Rapid Methods for Antimicrobial Resistance Diagnostics

Assessment of the aerobic glass beads fixed biofilm reactor (GBs-FBR) for the treatment of simulated methylene blue wastewater

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