Surface-Enhanced Raman Scattering Spectra of Thioflavin T: Manifestation of the Electromagnetic and Molecular Enhancement Mechanisms

Маскевич, А. А.; Lavysh, A. V.; Kivach, L. N.; Маскевич, С. А.

doi:10.1007/s10812-015-0142-8

Cited by 5 publications

(6 citation statements)

References 21 publications

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“…This result supports the formation of aggregates enriched in β‐sheets only in DLB‐seeded reactions, which is in accord with the positive seeding response of the RT‐QuIC. Importantly, because ThT affects the Raman signal, all RT‐QuIC reactions were prepared without adding ThT. Therefore, the observed increase in the β‐sheets content mirrors the RT‐QuIC results, providing an unbiased validation of the formation of aggregates during the course of the reaction.…”

Section: Discussionmentioning

confidence: 77%

Seeding variability of different alpha synuclein strains in synucleinopathies

Candelise

Schmitz

Llorens

et al. 2019

Annals of Neurology

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Objectives: Currently, the exact reasons why different α-synucleinopathies exhibit variable pathologies and phenotypes are still unknown. A potential explanation may be the existence of distinctive α-synuclein conformers or strains. Here, we intend to analyze the seeding activity of dementia with Lewy bodies (DLB) and Parkinson's disease (PD) brain-derived α-synuclein seeds by real-time quaking-induced conversion (RT-QuIC) and to investigate the structure and morphology of the α-synuclein aggregates generated by RT-QuIC. Methods: A misfolded α-synuclein-enriched brain fraction from frontal cortex and substantia nigra pars compacta tissue, isolated by several filtration and centrifugation steps, was subjected to α-synuclein/RT-QuIC analysis. Our study included neuropathologically well-characterized cases with DLB, PD, and controls (Ctrl). Biochemical and morphological analyses of RT-QuIC products were conducted by western blot, dot blot analysis, Raman spectroscopy, atomic force microscopy, and transmission electron microscopy. Results: Independently from the brain region, we observed different seeding kinetics of α-synuclein in the RT-QuIC in patients with DLB compared to PD and Ctrl. Biochemical characterization of the RT-QuIC product indicated the generation of a proteinase K-resistant and fibrillary α-synuclein species in DLB-seeded reactions, whereas PD and control seeds failed in the conversion of wild-type α-synuclein substrate. Interpretation: Structural variances of α-synuclein seeding kinetics and products in DLB and PD indicated, for the first time, the existence of different α-synuclein strains in these groups. Therefore, our study contributes to a better understanding of the clinical heterogeneity among α-synucleinopathies, offers an opportunity for a specific diagnosis, and opens new avenues for the future development of strain-specific therapies. ANN NEUROL 2019;85:691-703 S ynucleinopathies, such as Parkinson's disease (PD) and dementia with Lewy bodies (DLB), are a class of neurodegenerative diseases characterized by the presence of insoluble intraneural deposits consisting of fibrillary α-synuclein, 1,2 referred to as Lewy bodies. A PD with dementia (PDD) diagnosis is appropriate when the cognitive symptoms View this article online at wileyonlinelibrary.com.

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Section: Discussionmentioning

confidence: 77%

Seeding variability of different alpha synuclein strains in synucleinopathies

Candelise

Schmitz

Llorens

et al. 2019

Annals of Neurology

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“…Although signal loss can be seen for several signature peaks, the 1,601‐cm −1 peak showed the clearest gradual reduction with increasing peptide content (Figure b). Previous studies demonstrated that ThT SERS and fluorescence signals are very sensitive to the physicochemical properties of the surrounding medium . The underlying reason in the current case is suspected to be the hindrance exerted by the addition of intercalating beta‐rich peptide molecules around the dye, which can reduce the probability of several vibrational modes of the ThT molecule.…”

Section: Resultsmentioning

confidence: 77%

“…To demonstrate the use of MNS substrates for SERS‐based biosensing, ThT was chosen, as it is known to specifically intercalate within the beta sheets of Aβ(1–42)‐rich amyloid plaques . The molecule also demonstrates specific Raman peaks when covalently attached to plasmonic metal nanoparticles . The potential variation of ThT SERS intensities upon Aβ(1–42) interaction forms the basis of this biosensing platform, as similar complementary/specific interactions have been utilized to detect trace amounts of target biomolecules that causes alterations in the probe molecule‐originated SERS signal .…”

Section: Resultsmentioning

confidence: 99%

“…Thioflavin‐T (ThT) is a widely used fluorescent molecule to diagnose the presence of senile plaques in AD, where a significant fluorescence enhancement of the dye occurs upon incorporating into the beta sheet‐rich structures of the amyloid fibrils . Moreover, the molecule can covalently attach to metal surfaces that allow the formation of intensified characteristic ThT SERS signals …”

Section: Introductionmentioning

confidence: 99%

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Fabrication of thioflavin‐T‐modified nanopillared SERS substrates for ultrasensitive beta‐amyloid peptide detection

Altuntaş

Büyükserin

2018

J Raman Spectroscopy

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Alzheimer disease (AD) is a major cause of death in the world, and despite intense efforts, there is still no cure for this neurodegenerative disorder. Therefore, early diagnosis of AD is of prime importance, and extensive studies are being conducted to improve the current early detection capabilities and to identify new potential biomarkers for this disease. Here in, we report the fabrication of a novel nanobiosensor platform for the surface‐enhanced Raman spectroscopy (SERS)‐based ultrasentisitive detection of beta‐amyloid (1–42) peptide (Aβ[1–42]), a well‐established biomarker of AD. Polymeric films having multibranched nanopillared surfaces (MNS) were first fabricated by drop‐casting polycarbonate solutions onto anodized aluminum oxide molds that possess hierarchically branched pores. After peeling from the nanoporous molds, a 20‐nm Au coating allowed these MNS substrates to sense sub‐picomolar levels of Thioflavin‐T (ThT), a SERS‐active dye, clinically used to diagnose amyloid plaque presence. Of particular interest, the ThT‐modified MNS films demonstrated a gradual ThT SERS signal suppression when incubated with increasing concentrations of Aβ(1–42), and this trend was used to obtain a limit of detection value of 0.5 pg/ml and a linear dynamic range between 0.5 pg/ml and 100 ng/ml. The developed biosensor platform has stable ThT SERS signals for extended periods and displayed promising results in artificial saliva. Ongoing studies are focused on its potential with real body fluids.

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“…Surface-enhanced Raman spectroscopy (SERS) is a powerful method to gather nanoscale vibrational information about molecules close to a surface. [1][2][3] Applications can be found, among others, in bio-chemistry, 4,5 forensic sciences, 6,7 and catalysis. 8,9 Common SERS substrates are roughened silver electrodes and metallic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Fast High-Resolution Screening Method for Reactive Surfaces by Combining Atomic Force Microscopy and Surface-Enhanced Raman Scattering

Hoorn¹,

Wessels²,

Ariese³

et al. 2016

Appl Spectrosc

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A fast high-resolution screening method for reactive surfaces is presented. Atomic force microscopy (AFM) and surface-enhanced Raman spectroscopy (SERS) are combined in one method in order to be able to obtain both morphological and chemical information about processes at a surface. In order to accurately align the AFM and SERS images, an alignment pattern on the substrate material is exploited. Subsequent SERS scans with sub-micron resolution are recorded in 30 min per scan for an area of 100 × 100 µm and are accompanied by morphological information, supplied by a fast AFM, of the same area. Hence, a complete reactivity overview is obtained within several hours with only a monolayer of reactant. To demonstrate the working principle of this method, a SERS substrate containing the alignment pattern and silver nanoparticle aggregates as catalytic sites is prepared to study the photo-catalytic reduction of p-nitrothiophenol ( p-NTP).

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Surface-Enhanced Raman Scattering Spectra of Thioflavin T: Manifestation of the Electromagnetic and Molecular Enhancement Mechanisms

Cited by 5 publications

References 21 publications

Seeding variability of different alpha synuclein strains in synucleinopathies

Seeding variability of different alpha synuclein strains in synucleinopathies

Fabrication of thioflavin‐T‐modified nanopillared SERS substrates for ultrasensitive beta‐amyloid peptide detection

Fast High-Resolution Screening Method for Reactive Surfaces by Combining Atomic Force Microscopy and Surface-Enhanced Raman Scattering

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