Surface-enhanced Raman scattering (SERS) spectroscopy technique for lactic acid in serum measurement

Chiang, Hui Hua Kenny; Hsu, Po Hsiang

doi:10.1117/12.618127

Cited by 3 publications

(1 citation statement)

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“…Therefore, silver colloid was employed to enhance the Raman signal of lactic acid at physiological concentrations. The SERS spectra of 10 -3 M lactic acid revealed ten well distinguished SERS peaks 26 . The SERS and Raman characteristic peaks of lactic acid are quite different.…”

Section: Resultsmentioning

confidence: 99%

In vivo blood lactic acid monitoring using microdialysis and surface-enhanced Raman spectroscopy

2008

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Blood lactic acid concentration is an important indicator for physiological functions. To develop a rapid and sensitive measurement technique for monitoring blood lactic acid may provide a useful tool in clinical diagnosis. We proposed to develop a microdialysis surface-enhanced Raman spectroscopy (microdialysis-SERS) approach to filter/reduce interference from other large metabolites in blood and enhance the detection sensitivity for blood lactic acid. In this study, a microdialysis probe was constructed using 13 kDa cut-off dialysis membrane. The dialysate was mixed with 50 nm Ag colloidal nanoparticles automatically in a micro-fluid chamber for SERS detection under blood microdialysis of Sprague-Dawley rat. The linear range of SERS-lactic acid measurement is 10 -5~3 x10 -4 M with R 2 value of 0.99. The optimal mixing flow rate of nanoparticles is 18 µl/min under microdialysis at constant flow rate (2 µl/min). Real time lactic acid monitoring in vivo also has been demonstrated using microdialysis-SERS system.

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Section: Resultsmentioning

confidence: 99%